Analysis of arctic char (Salvelinus alpinus) haplotypes in the largest lakes of the North-West Region of Russia for genetic monitoring

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Abstract

BACKGROUND: Arctic char is a residential form of the Arctic char Salvelinus alpinus and a commercial target in Ladoga Lake. Caused by fishing the gradual decline in the number of ones in the largest lakes of the North-Western Region of Russia is compensated by measures for its artificial reproduction.

AIM: The aim of this work was to carry out gaplotyping of the arctic char (Salvelinus alpinus) population in the largest lakes of the northwestern region of Russia.

MATERIALS AND METHODS: The gaplotyping by PCR amplification in combination with restriction fragment length polymorphism (PCR-RFLP) of several amplified mtDNA regions, sequencing of the D-loop of mtDNA.

RESULTS: Screening studies of the genetic diversity of arctic char were carried out in three geographical population of the North-Western region of Russia and also the lake form of Arctic char from Sobachye Lake (Putorana Plateau, Western Siberia). The variability of a significant part (more than 35%) of the mitochondrial genome was analyzed including the regions of 16S rRNA/ND1/ND2 (2000 bp), COXI (567 bp), ND5/ND6 (2490 bp) and D-loop (1097 bp). Analysis of the nucleotide sequences of the control region mtDNA revealed 6 different haplotypes that were included in a single Eurasian group of char.

CONCLUSIONS: The panel of PCR-RFLP markers can serve as a tool for monitoring the genetic structure of the arctic char populations of Ladoga Lake under conditions of ones artificial maintenance.

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About the authors

Marina N. Kiseleva

St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography

Email: marina.marinakisel@yandex.ru
SPIN-code: 5436-3126
Russian Federation, Saint Petersburg

Diana K. Mitryushkina

St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography

Email: dianaa.5@yandex.ru
SPIN-code: 4546-5942
Russian Federation, Saint Petersburg

Tatiana A. Filatova

St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography; St. Petersburg State University of Veterinary Medicine

Email: tanechka23.9292@mail.ru
SPIN-code: 9998-1684
Russian Federation, Saint Petersburg; Saint Petersburg

Vasily A. Golotin

St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography; Academician I.P. Pavlov First St. Petersburg State Medical University

Author for correspondence.
Email: golotin@bk.ru
ORCID iD: 0000-0003-0385-2463
SPIN-code: 7198-3170
Scopus Author ID: 56641547400

Cand. Sci. (Biology)

Russian Federation, Saint Petersburg; Saint Petersburg

Alina A. Zhukova

St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography; Herzen State Pedagogical University of Russia

Email: gatteriyagreen@gmail.com
ORCID iD: 0000-0002-4416-1901
SPIN-code: 6849-0483

Cand. Sci. (Biology)

Russian Federation, Saint Petersburg; Saint Petersburg

Konstantin V. Rozhkovan

St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography

Email: 27.tomcat@gmail.com
ORCID iD: 0000-0002-8403-8342
SPIN-code: 2000-6703

Cand. Sci. (Biology)

Russian Federation, Saint Petersburg

Anastasia E. Mamaeva

St. Petersburg State University of Veterinary Medicine

Email: anastasiamamaeva43@gmail.com
Russian Federation, Saint Petersburg

Olga V. Apalikova

St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography

Email: olga_apalikova@mail.ru
ORCID iD: 0000-0002-1188-2446
SPIN-code: 6660-4950
Scopus Author ID: 6507160902

Cand. Sci. (Biology)

Russian Federation, Saint Petersburg

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2. Fig. 1. Consensus ML (a) and BI (b) dendrograms of mtDNA D-loop haplotypes of the dwelling form of Arctic char — Palia — of the Ladoga and Onega Lake system and close representatives of Arctic char of Scandinavia and Northern Siberia deposited in Genbank

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3. Fig. 2. Median network of mtDNA D-loop haplotypes of the dwelling form of the Arctic char – Palia — of the Ladoga and Onega Lake system and the close representatives of the Arctic char of Scandinavia and Northern Siberia. The number of nucleotide substitutions between haplotypes is shown by cross sections on the lines connecting the circles

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