Genotoxic effect of restraint and stress pheromone on somatic and germ cells of mouse males Mus musculus L.
- Authors: Shcherbinina V.D.1, Petrova M.V.2, Glinin T.S.1, Daev E.V.1,3
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Affiliations:
- Saint Petersburg State University
- Institute of Higher Nervous Activity and Neurophysiology of RAS
- Pavlov Institute of Physiology of the RAS
- Issue: Vol 19, No 2 (2021)
- Pages: 169-179
- Section: Genetic toxicology
- Submitted: 19.04.2021
- Accepted: 11.06.2021
- Published: 27.07.2021
- URL: https://journals.eco-vector.com/ecolgenet/article/view/65208
- DOI: https://doi.org/10.17816/ecogen65208
- ID: 65208
Cite item
Abstract
BACKGROUND: Different stressors affect the genome integrity, but the mechanisms of such action are underexplored.
MATERIALS AND METHODS: Bone marrow and testicular cells of CBA and CD-1 mouse males were used to estimate their genome integrity after stressor action by the comet assay.
RESULTS: It is shown here that restraint and 2,5-dimethylpyrazine both increase damaged cell frequency in bone marrow as well as in testes of mouse males. For the first time the effect of immobilization and 2,5-dimethylpyrazine in testicular cells is demonstrated using the comet assay. Both stressors have similar effects in cells of both tissues analyzed.
CONCLUSION: Mechanisms of the effects and possible role in microevolution are under discussion.
Keywords
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About the authors
Veronika D. Shcherbinina
Saint Petersburg State University
Email: sherbinina.veronika2014@yandex.ru
ORCID iD: 0000-0002-4813-736X
Graduate student
Russian Federation, Saint PetersburgMarina V. Petrova
Institute of Higher Nervous Activity and Neurophysiology of RAS
Email: mizopsilofit@inbox.ru
ORCID iD: 0000-0003-4385-7509
PhD, Cand. Sci. (Biol.)
Russian Federation, MoscowTimofey S. Glinin
Saint Petersburg State University
Email: t.glinin@gmail.com
ORCID iD: 0000-0003-0550-741X
SPIN-code: 2549-2759
PhD, Cand. Sci. (Biol.)
Russian Federation, Saint PetersburgEugene V. Daev
Saint Petersburg State University; Pavlov Institute of Physiology of the RAS
Author for correspondence.
Email: mouse_gene@mail.ru
ORCID iD: 0000-0003-2036-6790
PhD, Dr. Sci. (Biol.)
Russian Federation, Saint Petersburg; Saint PetersburgReferences
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