Genotoxic effect of restraint and stress pheromone on somatic and germ cells of mouse males Mus musculus L.

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Abstract

BACKGROUND: Different stressors affect the genome integrity, but the mechanisms of such action are underexplored.

MATERIALS AND METHODS: Bone marrow and testicular cells of CBA and CD-1 mouse males were used to estimate their genome integrity after stressor action by the comet assay.

RESULTS: It is shown here that restraint and 2,5-dimethylpyrazine both increase damaged cell frequency in bone marrow as well as in testes of mouse males. For the first time the effect of immobilization and 2,5-dimethylpyrazine in testicular cells is demonstrated using the comet assay. Both stressors have similar effects in cells of both tissues analyzed.

CONCLUSION: Mechanisms of the effects and possible role in microevolution are under discussion.

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About the authors

Veronika D. Shcherbinina

Saint Petersburg State University

Email: sherbinina.veronika2014@yandex.ru
ORCID iD: 0000-0002-4813-736X

Graduate student

Russian Federation, Saint Petersburg

Marina V. Petrova

Institute of Higher Nervous Activity and Neurophysiology of RAS

Email: mizopsilofit@inbox.ru
ORCID iD: 0000-0003-4385-7509

PhD, Cand. Sci. (Biol.)

Russian Federation, Moscow

Timofey S. Glinin

Saint Petersburg State University

Email: t.glinin@gmail.com
ORCID iD: 0000-0003-0550-741X
SPIN-code: 2549-2759

PhD, Cand. Sci. (Biol.)

Russian Federation, Saint Petersburg

Eugene V. Daev

Saint Petersburg State University; Pavlov Institute of Physiology of the RAS

Author for correspondence.
Email: mouse_gene@mail.ru
ORCID iD: 0000-0003-2036-6790

PhD, Dr. Sci. (Biol.)

Russian Federation, Saint Petersburg; Saint Petersburg

References

  1. Selye H. The physiology and pathology of exposure to stress. Montreal: Acta Med. Publ., 1950. 203 p.
  2. Chitty D. Population processes in the vole and their relevance to general theory. Can J Zool. 1960;38(1),99–113. doi: 10.1139/z60-011
  3. Hoffmann AA, Hercus MJ. Environmental stress as an evolutionary force. BioScience. 2000;50(3):217. doi: 10.1641/0006-3568(2000)050[0217: ESAAEF]2.3.CO;2
  4. Daev EV. Genetic aspects of stress neuroendocrinology. In: Neuroendocrinology research developments. Penkava NS, Haight LR, editors. NY: Nova Science Publishers, 2010. 119–133 p.
  5. Zhou P, Lian HY, Cui W, et al. Maternal-restraint stress increases oocyte aneuploidy by impairing metaphase I spindle assembly and reducing spindle assembly checkpoint proteins in mice. Biol Reprod. 2012;86(3):83. doi: 10.1095/biolreprod.111.095281
  6. Selye H. A syndrome produced by diverse nocuous agents. 1936. J Neuropsychiatry Clin Neurosci. 1998;10(2):230–231. doi: 10.1176/jnp.10.2.230a
  7. Owusu-Ansah E, Perrimon N. Stress signaling between organs in metazoa. Annu Rev Cell Dev Biol. 2015;31:497–522. doi: 10.1146/annurev-cellbio-100814-125523
  8. Daev EV. Stress, chemocommunication, and the physiological hypothesis of mutation. Russian Journal of Genetics. 2007;43(10):1082–1092. (In Russ.) doi: 10.1134/s102279540710002x
  9. Dayas CV, Buller KM, Crane JW, et al. Stressor categorization: acute physical and psychological stressors elicit distinctive recruitment patterns in the amygdala and in medullary noradrenergic cell groups. Eur J Neurosci. 2001;14(7):1143–1152. doi: 10.1046/j.0953-816x.2001.01733.x
  10. Bowers SL, Bilbo SD, Dhabhar FS, Nelson RJ. Stressor-specific alterations in corticosterone and immune responses in mice. Brain Behav Immun. 2008;22(1):105–113. doi: 10.1016/j.bbi.2007.07.012
  11. Novotny M, Jemiolo B, Harvey S, et al. Adrenal-mediated endogenous metabolites inhibit puberty in female mice. Science. 1986;231(4739):722–725. doi: 10.1126/science.3945805
  12. Koyama S. Primer effects by conspecific odors in house mice: a new perspective in the study of primer effects on reproductive activities. Horm Behav. 2004;46(3):303–310. doi: 10.1016/j.yhbeh.2004.03.002
  13. Daev EV, Vorob’ev KV, Shustova TI, et al. Genotipspetsificheskie izmeneniya nekotorykh funktsional’nykh pokazatelei immunokompetentnykh kletok u samtsov laboratornykh myshei v usloviyakh feromonal’nogo stressa. Russian Journal of Genetics. 2000;36(8):872–876.
  14. Glinin TS. Puti stabilizatsii i destabilizatsii genoma kletok kostnogo mozga myshi pri deistvii ol’faktornykh khemosignalov [dissertation]. Saint Petersburg: 2018. 175 p. (In Russ.)
  15. Dyuzhikova NA, Daev EV. Genome and stress-reaction in animals and humans. Ecological genetics. 2018;16(1):4–26. (In Russ.) doi: 10.17816/ecogen1614-26
  16. Dobrzyńska MM. The effects in mice of combined treatments to X-rays and antineoplastic drugs in the Comet assay. Toxicology. 2005;207(2):331–338. doi: 10.1016/j.tox.2004.10.002
  17. Higashimoto M, Isoyama N, Ishibashi S, et al. Preventive effects of metallothionein against DNA and lipid metabolic damages in dyslipidemic mice under repeated mild stress. J Med Invest. 2013;60(3–4):240–248. doi: 10.2152/jmi.60.240
  18. Durnev AD, Zhanataev AK, Anisina EA, et al. Primenenie metoda shchelochnogo gel’-ehlektroforeza izolirovannykh kletok dlya otsenki genotoksicheskikh svoistv prirodnykh i sinteticheskikh soedinenii: metodicheskie rekomendatsii. Moscow: 2006. 27 p. (In Russ.)
  19. Fischman HK, Pero RW, Kelly DD. Psychogenic stress induces chromosomal and DNA damage. Int J Neurosci. 1996;84(1–4): 219–227. doi: 10.3109/00207459608987267
  20. El-Refaiy AI, El-Desouki NI, Abdel-Azeem H, Elbaely MM. Cytogenetical study on the Effect of Immobilization Stress on Albino Rat and the Ameliorative Role of Diazepam. Current Science International. 2017;6(4):900–907.
  21. Malvandi AM, Haddad F, Moghimi A. Acute restraint stress increases the frequency of vinblastine-induced micronuclei in mouse bone marrow cells. Stress. 2010;13(3):276–280. doi: 10.3109/10253890903296710
  22. Hara MR, Kovacs JJ, Whalen EJ, et al. A stress response pathway regulates DNA damage through β2-adrenoreceptors and β-arrestin-1. Nature. 2011;477(7364):349–353. doi: 10.1038/nature10368
  23. Mikhailov VM, Vezhenkova IV. Double-strand breaks of DNA of C57BL and mdx mouse cardiomyocytes after dynamic stress. Cell Tiss Biol. 2007;1:328–333. doi: 10.1134/S1990519X07040049
  24. Aguilera A, García-Muse T. Causes of genome instability. Annu Rev Genet. 2013;47:1–32. doi: 10.1146/annurev-genet-111212-133232
  25. Daev EV, Dukelskaya AV. The female pheromone 2,5-dimethylpyrazine induces sperm-head abnormalities in male CBA mice. Russian Journal of Genetics. 2003;39(7):811–815. doi: 10.1023/A:1024709321888
  26. Daev EV, Dukelskaya AV. Induction of meiotic disturbances in spermatocytes i by pheromones as an inhibiting mechanism of male reproductive function in house mice. Cell and Tissue Biology. 2005;47(6):505–509. (In Russ.)
  27. Daev EV, Petrova MV, Onopa LS, et al. DNA damage in bone marrow cells of mouse males in vivo after exposure to the pheromone: Comet assay. Russian Journal of Genetics. 2017;53(10): 1105–1112. (In Russ.) doi: 10.1134/s1022795417100027
  28. Jemiolo B, Novotny M. Inhibition of sexual maturation in juvenile female and male mice by a chemosignal of female origin. Physiol Behav. 1994;55(3):519–522. doi: 10.1016/0031-9384(94)90110-4
  29. Borodin PM, Belyaev DK. Vliyanie stressa na chastotu krossingovera vo 2-i khromosome domovoi myshi. Doklady AN SSSR. 1980;253(3):727–729. (In Russ.)
  30. Borodin PM, Belyaev DK. Vliyanie ehmotsional’nogo stressa na chastotu rekombinatsii v 1-i khromosome domovoi myshi. Doklady AN SSSR. 1986;286(3):726–728. (In Russ.)
  31. Lobashev ME. Fiziologicheskaya (paranekroticheyskaya) gipoteza mutatsionnogo protsessa. Pushkin Leningrad state university journal. 1947;8:10–29. (In Russ.)
  32. Markel AL. Behavior, stress, and evolution. Filosofiya nauki. 2013;56(1):140–152. (In Russ.)
  33. Hodes GE, Pfau ML, Leboeuf M, et al. Individual differences in the peripheral immune system promote resilience versus susceptibility to social stress. PNAS USA. 2014;111(45):16136–16141. doi: 10.1073/pnas.1415191111
  34. Jiang W, Li Y, Wei W, et al. Spleen contributes to restraint stress induced hepatocellular carcinoma progression. Int Immunopharmacol. 2020;83:106420. doi: 10.1016/j.intimp.2020.106420
  35. Acevedo-Whitehouse K, Duffus AL. Effects of environmental change on wildlife health. Philos Trans R Soc Lond B Biol Sci. 2009;364(1534):3429–3438. doi: 10.1098/rstb.2009.0128
  36. Brown RE. Mammalian Social Odors: A Critical Review. Adv Study Behav. 1979;10:104–143. doi: 10.1016/S0065-3454(08)60094-7
  37. Brennan PA, Kendrick KM. Mammalian social odours: attraction and individual recognition. Philos Trans R Soc Lond B Biol Sci. 2006;361(1476):2061–2078. doi: 10.1098/rstb.2006.1931

Supplementary files

Supplementary Files
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1. Fig. 1. Degree of DNA damage in testes cells of male CD-1 mice after exposure to two different stressors (Me; Range: Min to Max)

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2. Fig. 2. Exponential distribution of damaged testicular cells accordingly DNA content in comet tails (Y0 e(–KX); 95%CI) of mouse males of control group (H2O) (1), and positive control group (males injected with mitomycin C) (2). Parameters Y0 and F0.5 are specified. Data from the first experiment

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3. Fig. 3. Frequency distribution of testicular cells with different DNA content in comet tails (Y0e(–KX); 95%CI) after 2 hrs exposure of CD-1 mouse males to different stressors. 1 – control; 2 – restraint stress; 3 – 2,5-dimethylpyrazine

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4. Fig. 4. Degree of DNA damage in bone marrow cells of male CBA mice after exposure to two different stressors (Me; Range: Min to Max)

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5. Fig. 5. Frequency distribution of bone marrow cells with different DNA content in comet tails after 2 hrs exposure of CBA mouse males to different stressors. 1 – control; 2 – restraint stress; 3 –2,5-dimethylpyrazine

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