Gut microbiota of healthy and saprolegniosis-affected whitefish (Coregonus nasus) producers in aquaculture



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Abstract

Background. The gut microbiota is one of the main factor influencing the immunity of animals, including fish. Understanding the taxonomic and quantitative composition of the microbiota may hold the key to enhance fish immunity against bacterial and fungal infections in aquaculture that potentially could optimize fish farm management. The aim of this work is to determine the taxonomic composition differences of the gut microbiome between healthy and Saprolegnia-affected breeders of broad whitefish (Coregonus nasus, Pallas, 1776) under industrial farming conditions using high-throughput sequencing. Materials and methods. The gut microbiota composition was analyzed using NGS sequencing and the methods of bioinformatics. Hematological profiling was performed using standard microscopy-based techniques. Results. Preliminary data from high-throughput molecular genetic screening of the gut microbiota diversity in healthy and Saprolegnia-affected C. nasus individuals during the spawning period are presented. The hematological profile reliably distinguishes clinical differences between healthy fish and those infected with Saprolegnia. Differences in the taxonomic and quantitative composition of the gut microbiomes in C. nasus has been shown. Conclusion. Fish with the clinical signs of saprolegniosis showed a dominance of the phylum Proteobacteria (on average of 45.3%). There were comparable propotions (26.67–30.27%) of Proteobacteria, Firmicutes, and Actinobacteriota observed in healthy fish.

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About the authors

Maxim Vylka

Санкт-Петербургский филиал ГНЦ РФ ФГБНУ "ВНИРО"("ГосНИОРХ" им. Л.С. Берга")

Email: vylka.maxim@yandex.ru
ORCID iD: 0000-0001-7583-864X
SPIN-code: 5468-2834
Russian Federation

Anatole Lyutikov

St. Petersburg Branch of VNIRO ("GosNIORKh" named after L.S. Berg)

Email: tokmo@mail.ru
ORCID iD: 0000-0003-0418-8218
SPIN-code: 9187-6075
Russian Federation

Svetlana Dyakova

St. Petersburg Branch of VNIRO (GosNIORKh named after L.S. Berg)

Email: dyakova@niorh.vniro.ru
ORCID iD: 0000-0001-9970-403X
SPIN-code: 4202-4471
Russian Federation

Denis Karlov

Email: ds.karlov@arriam.ru
ORCID iD: 0000-0002-9030-8820
SPIN-code: 8355-8091

Alina A. Zhukova

St. Petersburg branch of Russian Federal Research Institute of Fisheries and Oceanography; Herzen State Pedagogical University of Russia

Email: gatteriyagreen@gmail.com
ORCID iD: 0000-0002-4416-1901
SPIN-code: 6849-0483

Cand. Sci. (Biology)

Russian Federation, Saint Petersburg; Saint Petersburg

Vasily Golotin

Russian Federal Research Institute of Fisheries and Oceanography

Author for correspondence.
Email: golotin@bk.ru
ORCID iD: 0000-0003-0385-2463
SPIN-code: 7198-3170
Russian Federation

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