Polymorphism of the cytochrome P450 CYP1A1 (Ile462Val) gene in the populations of Balkars and Karachays

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Abstract

BACKGRAUND: Cytochrome P450 is an enzyme involved in the metabolism of phase I xenobiotics, toxins, endogenous hormones and pharmaceuticals.

AIM: Is studying the polymorphism of the CYP1A1 gene (Ile462Val, rs1048943) in the Turkic-speaking populations of the central part of the North Caucasus Region (Balkars and Karachays).

MATERIALS AND METHODS: We analyzed DNA isolated from leukocytes of peripheral venous blood of a total of 177 unrelated Balkars and Karachays (104 Balkars, 73 Karachays).

RESULTS: The ethnicity of the studied individuals was established, indicating their ancestors up to the third generation. The method of genotyping was performed by real-time PCR using competing TaqMan probes. As a result: the frequency of the 462Val variant in the sample of Balkars was 8,6% (95% CI 5,21–13,33), slightly above the frequency range found in European populations. In the sample of Karachays, the frequency of the 462Val allele was 7,5% (95% CI 9,82–13.08), which is the upper limit of values typical for European populations.

CONCLUSION: In the Turkic-speaking populations of the central part of the North Caucasus, the CYP1A1 462Val allele occurs with a frequency characteristic of the Near Eastern and European populations.

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About the authors

Murat A. Dzhaubermezov

Bashkir State University; Institute of Biochemistry and Genetics

Email: murat-kbr@mail.ru
SPIN-code: 1066-3369

Cand. Sci. (Biol.)

Russian Federation, 450076, Republic of Bashkortostan, Ufa, st. Zaki Validi, 32; 450054, Republic of Bashkortostan, Ufa, Prospekt Oktyabrya, 71

Elvira T. Mingazheva

Bashkir State University; Institute of Biochemistry and Genetics

Email: elvira.f91@mail.ru

Assistant

Russian Federation, 450076, Republic of Bashkortostan, Ufa, st. Zaki Validi, 32; 450054, Republic of Bashkortostan, Ufa, Prospekt Oktyabrya, 71

Darya S. Axberg

Bashkir State University

Email: dasha_00_19@mail.ru

student

Russian Federation, 450076, Republic of Bashkortostan, Ufa, st. Zaki Validi, 32

Natalya V. Ekomasova

Bashkir State University; Institute of Biochemistry and Genetics

Email: trofimova_nata_@mail.ru
SPIN-code: 6528-4117

Cand. Sci. (Biol.)

Russian Federation, 450076, Republic of Bashkortostan, Ufa, st. Zaki Validi, 32; 450054, Republic of Bashkortostan, Ufa, Prospekt Oktyabrya, 71

Elsa K. Khusnutdinova

Bashkir State University; Institute of Biochemistry and Genetics

Author for correspondence.
Email: elzakh@mail.ru
SPIN-code: 7408-9797
Scopus Author ID: 35381528600

Dr. Sci. (Biol.)

Russian Federation, 450076, Republic of Bashkortostan, Ufa, st. Zaki Validi, 32; 450054, Republic of Bashkortostan, Ufa, Prospekt Oktyabrya, 71

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2. Figure 1. Map of residence and sampling sites for the Karachays and Balkars biomaterials

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