Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

Cover Page
Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract


Background. The spectrum of cytogenetic abnormalities in the apical root meristem of Elodea сanadensis Michx. (elodea) from the Yenisei River was studied depending on the type of anthropogenic pollution. We compared elodea samples from areas with radiation-contaminated sediments (area affected by Rosatom Mining and Chemical Combine, MCC, Zheleznogorsk) and areas with chemical pollution (Krasnoyarsk). Materials and methods. The apical root meristem of elodea was fixed in acetic alcohol (96 % ethanol and glacial acetic acid, 3 : 1) and stained with 1 % aceto-orcein. The cytogenetic analysis of metaphase and ana-telophase elodea cells was carried out with temporal squashed preparations using an Olympus CX31 microscope. Results. At metaphase stage, the predominant types of abnormalities in elodea cells were disoriented chromosomes (up to 9 % of total metaphase cells) and chromosome agglutination (6 %). In the area affected by the MCC an increased content of ring chromosomes in elodea cells was detected, but in terms of frequency of their occurrence no significant differences were revealed between samples from areas with different types of pollution. Among abnormalities at ana-telophase stage, bridges (to 20% of dividing cells) and disoriented chromosomes (up to 8%) dominated. The following abnormalities were also detected: multipolar and asymmetrical mitoses, agglutination and cells with multiple chromosome abnormalities. It was shown that in areas with the highest content of 137Cs in bottom sediments the frequency of cells with bridges and multiple chromosome abnormalities significantly increased as compared to samples from other areas. Conclusion. In the radioactive contamination area of the Yenisei River the spectrum of abnormalities was dominated by chromosome aberrations (bridges, agglutination, chromosome fragmentation) and multiple abnormalities in one and the same cell. In areas with non-radioactive (chemical) contamination of sediments, mitotic spindle irregularities prevailed.

Full Text

Restricted Access

About the authors

Marina Yu Medvedeva

Institute of Biophysics SB RAS

Email: medvedeva_m79@mail.ru
Junior Researcher (Ph.D), Laboratory of radioecology

Alexander Ya Bolsunovsky

Institute of Biophysics SB RAS

Email: radecol@ibp.ru
Head of laboratory (Sc.D), Laboratory of radioecology

References

  1. Алиева И.Б., Воробьев И.А. Поведение клеток и распределение центриолей при многополюсном митозе, индуцированном действием нокодазола // Цитология. - 1989. - Т. 31. - Вып. 6. - С. 633-637. [Aliyeva IB, Vorobyov IA. Povedenije kletok i raspredelenie centriolej pri mnogopoljusnom mitoze, inducirovannom dejstviem nokodazola. Cytologia. 1989;31(6):633-637. (In Russ.)].
  2. Алов И.А. Цитофизиология и патология митоза. - М.: Медицина, 1972. [Alov IA. Cytophysiology and pathology of a mitosis. Moscow: Medicine; 1972. (In Russ.)].
  3. Артюхов В.Г., Калаев В.Н. Цитогенетический мониторинг состояния окружающей среды на территориях, подвергшихся радиоактивному загрязнению в результате аварии на Чернобыльской АЭС // Радиационная биология. Радиоэкология. - С. 2006. - Т. 46. - Вып. 2. - С. 208-215. [Artyukhov VG, Kalaev VN. The Cytogenetic Monitoring of the Environmental Conditions on the Territories Exposed by the Radioactive Contamination as a Result of Chernobyl Nuclear Power Station Accident. Radiatsionnaya biologiya. Radioekologiya. 2006; 46(2):208-215 (In Russ.)].
  4. Буторина А.К., Калаев С.С., Карпова В.Н. Цитологические нарушения в соматических клетках человека и березы повислой в районах г. Воронежа с различной интенсивностью антропогенного загрязнения // Экология. - 2002. - Т. 33. - Вып. 6. - С. 438-441. [Butorina AK, Kalaev VN, Karpova SS. Cytogenetic damage of Human somatic cells and weeping Birch cells in Voronezh districts with different levels of anthropogenic pollution. Russian Journal of Ecology. 2002;33(6):413-416. (In Russ.)]
  5. Болсуновский А.Я., Муратова Е.Н., Суковатый А.Г., и др. Радиоэкологический мониторинг реки Енисей и цитогенетические характеристики водного растения Elodea сanadensis // Радиационная биология. Радиоэкология. - 2007. - T. 47. - Вып. 1. - C. 63-73. [Bolsunovsky AYa, Muratova EN, Sukovaty AG, et al. Radioecologichesky monitoring reki Enisjej i citogeneticheskije kharakteristiki vodnogo rastenija Elodea сanadensis. Radiacionnaja biologija. Radioekologija. 2007; 47(1):63-73 (In Russ.)].
  6. Гланц С. Медико-биологическая статистика. - М.: Практика, 1998. - 459 с. [Glants S. Mediko-biologicheskaya statistika. Moscow: Praktika; 1998: 459 (In Russ.)]
  7. Горячкина О.В., Сизых О.А. Цитогенетические реакции хвойных растений в антропогенно нарушенных районах г. Красноярска и его окрестностей // Хвойные бореальной зоны. - 2012. - Т. 30. - Вып. 1-2. - С. 46-51. [Goryachkina OV, Sizyh OA. Citogeneticheskije reakcii hvojnyh rastenij v antropogenno narushennyh rajonah g. Krasnojarska i ego okrestnostej. Hvojnye boreal’noj zony. 2012;30(1-2): 46-51 (In Russ.)]
  8. Зотина Т.А. Фитомасса и видовое разнообразие макрофитной растительности реки Енисей на участке от г. Красноярска до устья р. Ангара // Журнал Сибирского федерального университета. Серия Биология. - 2014. - Т. 7. - Вып. 1. - С. 73-86 [Zotina TA. Phytomass and Specific Diversity of Macrophyte Vegetation in the Middle Reach of the Yenisei River. J of Siberian Federal University. Biology series, 2014;7(1):73-86. (In Russ.)]
  9. Машкина О.С., Калаев В.Н., Мурая Л.С., Леликова Е.С. Цитогенетические реакции семенного потомства сосны обыкновенной на комбинированное антропогенное загрязнение в районе Новолипецкого металлургического комбината // Экологическая генетика. - 2009. - Т. 7. - Вып. 3. - С. 17-29. [Mashkina OS, Kalayev VN, Muraya LS, Lelikova ES. Cytogenetic response of seed progeny of scots pine to combined anthropogenic pollution in the area of Novolipetsk metallurgical combine. Ekologicheskaja genetika. 2009;7(3):17-29 (In Russ.)]
  10. Муратова Е.Н., Горячкина О.В., Корнилова М.Г., и др. Цитогенетическое изучение водных растений акватории Енисея в зоне радиационного загрязнения // Известия РАН. Серия биологическая. - 2004. - Вып. 5. - С. 510-517. [Muratova EN, Goryachkina OV, Kornilova MG, et al. Cytogeneticheskoje izuchenije vodnyh rastenij akvatorii Yeniseja v zonje radiacionnogo zagrjasnjenija. Izvestija RAN. Series biological. 2014;5:510-517 (In Russ.)]
  11. Севанькаев А.В., Потетня О.И., Михайлова Г.Ф. Частота цитогенетических нарушений в лимфоцитах периферической крови у жителей Орловской области, проживающих на загрязненных радионуклидами территориях после Чернобыльской аварии // Радиация и риск (Бюллетень Национального радиационно-эпидемиологического регистра). - 2003. - Вып. 1. - С. 87-95 [Sevankayev AV, Potetnya OI, Mikhaylova GF. Chastota citogeneticheskih narushenij v limfocitah perifericheskoj krovi u zhitelej Orlovskoj oblasti, prozhivajushhih na zagrjaznennyh radionuklidami territorijah posle Chernobyl’skoj avarii. Radiacija i risk. 2003; S1:87-95. (In Russ.)]
  12. Цитогенетический мониторинг: методы оценки загрязнения окружающей среды и состояния генетического аппарата организма. Учебное пособие / Калаев В.Н., Карпова С.С. - Воронеж: Изд-во ВГУ, 2004. - 79 c. [Citogenetichjesky monitoring: metody ocenki zagrjaznenija okruzhajuchej sredy i sostojanija geneticheskogo apparata organizma. Uchebnoe posobie. Kalayev V.N., Karpova C.C. Voronezh: Izdatjel’stvo VGU; 2004:79. (In Russ.)]
  13. Шевцова Н.Л., Гудков Д.И. Цитогенетические нарушения у тростника обыкновенного Phragmites australis в водоемах Чернобыльской зоны отчуждения // Гидробиологический журнал. - 2012. - T. 48. - Вып. 6. - С. 99-113 [Shevtsova NL, Gudkov DI. Cytogeneticheskije narushenija u trostnika obyknovjennogo Phragmites australis v vodoyomah Chernobyl’skoj zony otchuzhdenija. Hidrobiologichesky zhurnal. 2012;48(6):99-113 (In Russ).]
  14. Abdel Migit HMA, Azab YA, Ibrahim WM. Use of plant genotoxicity bioassay for the evaluation of efficiency of algal biofilters in bioremediation of toxic industrial effluent. Ecotoxicol. Environ. Safety. 2007;66(1):57-64. doi: 10.1016/j.ecoenv.2005.10.011.
  15. Al-Sabti K, Kureles B. Chromosomal aberration in onion (Allium cepa) induced by water chlorination by-products. Bull Environ Contam Toxicol. 1985;34(1):80-88. doi: 10.1007/bf01609706.
  16. Bolsunovsky A, Muratova E, Sukovaty A, Kornilova M. The effect of radionuclide and heavy metal contamination of the Yenisei River on cytogenetics of aquatic plant Elodea сanadensis. Radioprotection. 2009;44(5):83-88. doi: 10.1051/radiopro/20095021.
  17. Cabaravdic M. Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of cells to benzo(a)pyrene. Med Arh. 2010;64(4):215-218.
  18. Dudas M, Hlinka D, Jesenska R, Kalatova B. Tripolar mitosis in human cells and embryos: Occurrence, pathophysiology and medical implications. Acta Histochemica. 2015;117(1):111-125.
  19. Evseeva TI, Geras’kin SA, Shuktomova II. Genotoxicity and toxicity assay of water sampled from radium production industry storage cell territory by means of Allium-test. J Environ Radioact. 2003;68(3):235-248. doi: 10.1016/s0265-931x(03)00054-7.
  20. Fiskesjo G. The Allium-test - an alternative in environmental studies: the relative toxicity of metal ions. Mutation Research. 1988;197(2):243-260. doi: 10.1016/0027-5107(88)90096-6.
  21. Geras’kin S, Evseeva T, Oudalova A. Plants as a tool for the environmental health assessment. In: Encyclopedia of Environmental Health. Nriagu J. 2011a:571-579.
  22. Geras’kin S, Oudalova A, Michalik B, et al. Genotoxicity assay of sediment and water samples from the Upper Silesia post-mining areas Poland by means of Allium-test. Chemosphere. 2011b;83:1133-1146. doi: 10.1016/j.chemosphere.2011.01.008.
  23. Geras’kin SA, Oudalova AA, Dikarev VG, et al. Effects of chronic exposure in populations of Koeleria gracilis Pers. from the Semipalatinsk nuclear test site, Kazakhstan. J Environ Radioact. 2012;104:55-63. doi: 10.1016/j.jenvrad.2011.09.015.
  24. Liman R, Akyil D, Eren Y, Konuk M. Testing of the mutagenicity and genotoxicity of metolcarb by using both Ames/Salmonella and Allium test. Chemosphere. 2010;80(9):1056-61. doi: 10.1016/j.chemosphere.2010.05.011.
  25. MacDonald DD, Ingersoll CG, Berger T.A. Development and evaluation of consensus-based sediment quality guidelines for fresh-water ecosystems. Arch Environ Contam Toxicol. 2000;39(1):20-31. doi: 10.1007/s002440010075.
  26. Medvedeva MY, Bolsunovsky AY, Zotina TA. Cytogenetic abnormalities in aquatic plant Elodea canadensis in anthropogenic contamination zone of Yenisei River. Contemporary Problems of Ecology. 2014;7(4):422-432. doi: 10.1134/s1995425514040088.
  27. Mert M, Betül B. Cytogenetic effects of Urginea maritima L. aqueous extracts on the chromosomes by using Allium test method. Caryologia. 2008;61(4):342-348. doi: 10.1080/00087114.2008.10589645.
  28. Olorunfemi DI, Olorunfemi OP, Agbozu IE. Genotoxicity Assessment of Contaminated Drinking Water Sources in a Rural Community in Edo State of Nigeria. J of Geoscience and Environment Protection. 2014;2(2):52-59. doi: 10.4236/gep.2014.22009.
  29. Rodrigues AS, Oliveira NG, Gil OM, et al. Use of cytogenetic indicators in radiobiology. J Radiat Prot Dosim. 2005;115(1-4):455-460. doi: 10.1093/rpd/nci072.
  30. Seth CS, Misra V, Chauhan LKS, Singh RR. Genotoxicity of cadmium on root meristem cells of Allium cepa: cytogenetic and Comet assay approach. Ecotoxicol Environ Saf. 2008;71(3):711-716. doi: 10.1016/j.ecoenv.2008.02.003.
  31. Shevtsova NL, Gudkov DI. Cytogenic effects of long-term radiation on higher aquatic plants within the Chernobyl accident Exclusion Zone. Radioprotection. 2009;44(5): 937-940. doi: 10.1051/radiopro/20095167.
  32. Stevens JB, Liu G, Bremer SW, et al. Mitotic cell death by chromosome fragmentation. Cancer Res. 2007;67(16):7686-7694. doi: 10.1158/0008-5472.can-07-0472.
  33. Mustafa Y, Suna Arikan E. Genotoxicity testing of quizalofop-P-ethyl herbicide using the Allium cepa anaphase-telophase chromosome aberration assay. Caryologia. 2008;61(1):45-52. doi: 10.1080/00087114.2008.10589608.
  34. Yildiz M, Ciğerci IH, Konuk M, et al. Determination of genotoxic effects of copper sulphate and cobalt chloride in Allium cepa root cells by chromosome aberration and Comet assays. Chemosphere. 2009;75(7):934-938. doi: 10.1016/j.chemosphere.2009.01.023.

Supplementary files

Supplementary Files Action
1.
Fig. 2. The frequency of the occurrence of metaphase cells with chromosome agglutination and multiple abnormalities in apical root meristem of E. canadensis downstream of the Yenisei River (%, mean ± SE). Notations: * (values for multiple abnormalities, р ˂ 0.01), & (values for agglutination, р ˂ 0.005) significantly different from the sample of site Atamanovo, according to Student’s t-test

Download (70KB) Indexing metadata
2.
Fig. 4. The frequency of the occurrence of anatelophase cells with bridges in apical root meristem of E. canadensis downstream of the Yenisei River (%, mean ± SE). Notations: *, ** – the values significantly different from the sample of site Strelka (p ˂ 0.005), & – the values significantly different from the sample of site Atamanovo (р ˂ 0.005), according to Student’s t-test

Download (73KB) Indexing metadata

Statistics

Views

Abstract - 713

PDF (Russian) - 292

Cited-By


PlumX


Copyright (c) 2016 Medvedeva M.Y., Bolsunovsky A.Y.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies