Histological investigation of the fetal liver after propyphenazone administration

Abstract

Objective: Propyphenazone one of the pirazolon derivatives is widely used analgesic and antipyretic medicine. The purpose of this study was to evaluate propyphenazone effect on fetal liver after administered to maternal animal model.

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Methods: The experiment was conducted on Wistar breed rats. Propyphenazone was administered, in Tween 80 solution, using the stomach tube on days 8 to 14 of pregnancy at doses of 2.1 (PO), 21.0 (Pl), 210.0 mg/kg body weight (P2). Two control groups were done: T - receiving Tween 80, C — untreated control. The females were sacrificed on day 21 of gestation and the fetuses were delivered by cesarean section. The fetuses were sectioned in-situ and the fetal livers were taken for histological examination. The slides were examined using light microscopy after four stains: hematoxylin and eosin, silver Gomorii, van Giesson and histochemical by periodic acid-Schiff (paS).

Results: There were not internal and external macroscopic malformations. There were a adaptive changes and ecchymosis especially in group Pl. In the other groups, observation changes were not so often.

Conclusion: Those results suggest that propyphenazone may provide adaptive changes in fetal liver after short time application during pregnancy.