TNF-alpha and IL-I secretion in macrophage cultures obtained from placentas in various pregnancy outcomes
- Authors: Pavlov O.V.1, Selkov S.A.1, Arzhanova O.N.1, Selutin A.V.1, Shamugia M.S.1, Ananyeva V.V.1
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Affiliations:
- D.O. Ott Institute of Obstetrics and Gynecology
- Issue: Vol 48, No 5S (1999)
- Pages: 123-123
- Section: Articles
- Submitted: 20.02.2022
- Accepted: 20.02.2022
- Published: 15.12.1999
- URL: https://journals.eco-vector.com/jowd/article/view/101284
- DOI: https://doi.org/10.17816/JOWD101284
- ID: 101284
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Abstract
Objective: To investigate the role of inflammatory cytokines TNF-a, IL-I a and IL-I 13 in term and preterm parturition at different gestational ages.
Keywords
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Objective: To investigate the role of inflammatory cytokines TNF-a, IL-I a and IL-I 13 in term and preterm parturition at different gestational ages.
Methods: Placentas were obtained from nonlaboring women with cesarean deliveries at 21-26 weeks gestation (early preterm), 31-34 weeks gestation (late preterm), and term or from laboring women with spontaneous early preterm, late preterm abortions and vaginal delivery at term. Cell cultures were prepared by dispase-collagenase digestion of placenta tissue and flcoll-urografln density centrifugation. In vitro cytokine secretion was determined with ELISA.
Results: In nonlaboring group secretion ofTNF-a and IL-Ib 13 increased significantly since early to late preterm (TNF-a from 9,8±1,8 pg/ug cell protein to 20,1±6,9 pg/ng; IL-1(3 from 10,0±1,4pg/ug to 23,1±2,6 pg/ug). Increase of IL-a (30,5±6,1 pg/ug vs 17,9±2,1 pg/ug) and IL-lb 30,3 ±5,8 pg/ug) level was also observed in term compared with late preterm. TNF-a secretion did not change in term macrophages when compared with late term. Spontaneous laboring activity in early preterm led to 5-20-fold increase of cytokine secretion (TNF-a - 242,1±39,8 pg/u-g, IL-lb -86,4±7,0pg/ug, IL-a - 204,6±12,3 pg/ug). In contrast, in term laboring women we observed no significant changes in TNF-a production (19,0±3,8 pg/u-g) and even decreased IL-Ia and IL-Ib secretion (13,3±4,4 pg/ug and 15,4±1,6 pg/ug correspondingly).
Conclusion: Early preterm labors (second trimester of pregnancy) are associated with elevated placental cytokine production.
About the authors
O. V. Pavlov
D.O. Ott Institute of Obstetrics and Gynecology
Author for correspondence.
Email: info@eco-vector.com
Russian Federation, St. Petersburg
S. A. Selkov
D.O. Ott Institute of Obstetrics and Gynecology
Email: info@eco-vector.com
Russian Federation, St. Petersburg
O. N. Arzhanova
D.O. Ott Institute of Obstetrics and Gynecology
Email: info@eco-vector.com
Russian Federation, St. Petersburg
A. V. Selutin
D.O. Ott Institute of Obstetrics and Gynecology
Email: info@eco-vector.com
Russian Federation, St. Petersburg
M. S. Shamugia
D.O. Ott Institute of Obstetrics and Gynecology
Email: info@eco-vector.com
Russian Federation, St. Petersburg
V. V. Ananyeva
D.O. Ott Institute of Obstetrics and Gynecology
Email: info@eco-vector.com
Russian Federation, St. Petersburg