Factors influencing embryo development in-vitro

Abstract

Objective: The aim of this study was to summarize the results of the experimental data about factors influencing embryo morphology, the cleavage rate and early compactization in our program of assisted fertilization.

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Methods: The number of the blastomers, the presence of fragments and early compactization were evaluated through 72 hours after the oocytes retrival. The next factors were analyzed: the woman age (770 IVF cycles), the ovarian response to the induction of superovulation (1146 cycles), the hormonal ovaries insufficiency (466 cycles), the extent of sperm chromatin condensation (176 cycles) and the sperm morphology (85 cycles).

Results: The rate of embryo cleavage was decreased and blastomers fragmentation was increase with the increase of the woman age. The number of aspirated follicles correlated with improvement of embryo morphology and increased number of blastomers. The high rate of embryo fragmentation and the delay of the cleavage was observed in the women with anovulation. Patients with endometriosis had the reduced number and a severe fragmentation of the blastomers. The embryo development was impaired in women with hyperprolactinaemia. A flow cytometric test was proposed for the evolution of the extent of DNA deshielding in human sperm nuclei. Patients with abnormal sperm chromatin had less amount of embryos at the advanced stages of cleavage. The early embryo compactization did not depend on womanys age and the number of aspirated follicules. Such parameters of husbantys sperm as motility, percentage of morphologically normal and slightly amorphous cells were better in the group of women with early embryo compaction.

Conclusions: Embryo development dependends on the factors that reflect ovarian response to stimulation; maternal age and ovarian dysfunction influence the embryo quality; abnormal sperm chromatin disturbes cleavage. The early embryo compaction is influensed the sperm quality.