Email this article Serotonin receptor and serotonin transporter expressions in the placental villous tree in gestational diabetes mellitus
- Authors: Bettikher O.A.1,2, Belyaeva O.A.1, Dukovich A.I.1, Vorobeva O.M.1, Tral T.G.2, Tolibova G.K.2, Bart V.A.1, Kogan I.Y.1,2, Zazerskaya I.E.1,2
-
Affiliations:
- V.A. Almazov National Medical Research Center
- The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
- Issue: Vol 73, No 1 (2024)
- Pages: 5-16
- Section: Original Research
- URL: https://journals.eco-vector.com/jowd/article/view/562735
- DOI: https://doi.org/10.17816/JOWD562735
- ID: 562735
Cite item
Open Access
Access granted
Abstract
BACKGROUND: The serotonergic system during pregnancy plays an important role not only in carbohydrate metabolism, but also in the laying and regulation of the fetoplacental complex, growth and development of the fetus. The study of the expression of placental serotonin 5-HT2A receptor and serotonin transporter (SERT) in gestational diabetes mellitus is foremost for scrutinizing the pathogenesis of perinatal complications, as it may allow for finding new opportunities for their prevention and correction.
AIM: The aim of this study was to compare the expression patterns of the serotonin 5-HT2A receptor and SERT in placental tissue in gestational diabetes mellitus and in normal pregnancy.
MATERIALS AND METHODS: This comparative cohort study included pregnant women with gestational diabetes mellitus (n = 6) and patients with normal pregnancy (n = 10). The expression of serotonin 5-HT2A receptor (Abcam, USA) and SERT (Bioss Antibodies, USA) was studied in placenta samples from the both study groups by immunohistochemical method. Morphometric analysis was performed using the VideoTest-Morphology 5.2 program (Videotest Ltd., Russia).
RESULTS: The relative area of SERT expression in the placenta in gestational diabetes mellitus was higher compared to normal pregnancy (p < 0.001). The relative areas of expression of the serotonin 5-HT2A receptor in the placenta did not differ between the study groups (p = 0.5).
CONCLUSIONS: Higher SERT expression in the placentas of patients with gestational diabetes mellitus compared to those from women with normal pregnancies may reflect the level of tension of compensatory mechanisms in gestational diabetes mellitus and the effect of insulin therapy on these mechanisms.
Keywords
Full Text
About the authors
Ofelia A. Bettikher
V.A. Almazov National Medical Research Center; The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Author for correspondence.
Email: ophelia.bettikher@gmail.com
ORCID iD: 0000-0002-1161-1558
MD, Cand. Sci. (Med.)
Russian Federation, Saint Petersburg; Saint PetersburgOlga A. Belyaeva
V.A. Almazov National Medical Research Center
Email: belyaevaolga0138@gmail.com
ORCID iD: 0000-0002-6970-7085
MD
Russian Federation, Saint PetersburgAlbina I. Dukovich
V.A. Almazov National Medical Research Center
Email: alyadukovich@gmail.com
ORCID iD: 0000-0002-7912-035X
Russian Federation, Saint Petersburg
Olga M. Vorobeva
V.A. Almazov National Medical Research Center
Email: olgarasp@yandex.ru
ORCID iD: 0000-0002-1349-7349
MD, Cand. Sci. (Med.)
Russian Federation, Saint PetersburgTatiana G. Tral
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: ttg.tral@yandex.ru
ORCID iD: 0000-0001-8948-4811
MD, Cand. Sci. (Med.)
Russian Federation, Saint PetersburgGulrukhsor Kh. Tolibova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: gulyatolibova@yandex.ru
ORCID iD: 0000-0002-6216-6220
MD, Dr. Sci. (Med)
Russian Federation, Saint PetersburgVictor A. Bart
V.A. Almazov National Medical Research Center
Email: vbartvit@mail.ru
ORCID iD: 0000-0002-9406-4421
Cand. Sci. (Phys. & Math.)
Russian Federation, Saint PetersburgIgor Yu. Kogan
V.A. Almazov National Medical Research Center; The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: ikogan@mail.ru
ORCID iD: 0000-0002-7351-6900
MD, Dr. Sci. (Med.), Professor, Corresponding Member of the Russian Academy of Sciences
Russian Federation, Saint Petersburg; Saint PetersburgIrina E. Zazerskaya
V.A. Almazov National Medical Research Center; The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: zazera@almazovcentre.com
ORCID iD: 0000-0003-4431-3917
MD, Dr. Sci. (Med.), Professor
Russian Federation, Saint Petersburg; Saint PetersburgReferences
- Watts SW. 5-HT in systemic hypertension: foe, friend or fantasy? Clin Sci (Lond). 2005;108(5):399–412. doi: 10.1042/CS20040364
- Kanova M, Kohout P. Serotonin-its synthesis and roles in the healthy and the critically ill. Int J Mol Sci. 2021;22(9):4837. doi: 10.3390/ijms22094837
- Deroy K, Côté F, Fournier T, et al. Serotonin production by human and mouse trophoblast: involvement in placental development and function. Placenta. 2013;34(9). doi: 10.1016/j.placenta.2013.06.214
- Murthi P, Vaillancourt C. Placental serotonin systems in pregnancy metabolic complications associated with maternal obesity and gestational diabetes mellitus. Biochim Biophys Acta Mol Basis Dis. 2020;1866(2). doi: 10.1016/j.bbadis.2019.01.017
- Hadden C, Fahmi T, Cooper A, et al. Serotonin transporter protects the placental cells against apoptosis in caspase 3-independent pathway. J Cell Physiol. 2017;232(12):3520–3529. doi: 10.1002/jcp.25812
- Viau M, Lafond J, Vaillancourt C. Expression of placental serotonin transporter and 5-HT 2A receptor in normal and gestational diabetes mellitus pregnancies. Reprod Biomed Online. 2009;19(2):207–215. doi: 10.1016/s1472-6483(10)60074-0
- Blazevic S, Horvaticek M, Kesic M, et al. Epigenetic adaptation of the placental serotonin transporter gene (SLC6A4) to gestational diabetes mellitus. PLoS One. 2017;12(6). doi: 10.1371/journal.pone.0179934
- Li Y, Hadden C, Singh P, et al. GDM-associated insulin deficiency hinders the dissociation of SERT from ERp44 and down-regulates placental 5-HT uptake. Proc Natl Acad Sci USA. 2014;111(52):E5697–E5705. doi: 10.1073/pnas.1416675112
- Carrasco-Wong I, Moller A, Giachini FR, et al. Placental structure in gestational diabetes mellitus. Biochim Biophys Acta Mol Basis Dis. 2020;1866(2). doi: 10.1016/j.bbadis.2019.165535
- Tral TG, Tolibova GK, Musina EV, et al. Molecular and morphological peculiarities of chronic placental insufficiency formation caused by different types of diabetes mellitus. Diabetes mellitus. 2020;23(2):185–191. EDN: WMVKAO doi: 10.14341/DM10228
- Valencia-Ortega J, Saucedo R, Sánchez-Rodríguez MA, et al. Epigenetic alterations related to gestational diabetes mellitus. Int J Mol Sci. 2021;22(17). doi: 10.3390/ijms22179462
- Horvatiček M, Perić M, Bečeheli I, et al. Maternal metabolic state and fetal sex and genotype modulate methylation of the serotonin receptor type 2A gene (HTR2A) in the human placenta. Biomedicines. 2022;10(2):467. doi: 10.3390/biomedicines10020467
- Pavlova TV, Kaplin AN, Goncharov IYu, et al. Uteroplacental blood flow in maternal diabetes mellitus. Arkhiv Patologii. 2021;83(1):25–30. EDN: RTLQNV doi: 10.17116/patol20218301125
- Brodowski L, Rochow N, Yousuf EI, et al. The impact of parity and maternal obesity on the fetal outcomes of a non-selected Lower Saxony population. J Perinat Med. 2021;50(2):167–175. doi: 10.1515/jpm-2020-0614
- Anderson MS, Flowers-Ziegler J, Das UG, et al. Glucose transporter protein responses to selective hyperglycemia or hyperinsulinemia in fetal sheep. Am J Physiol Regul Integr Comp Physiol. 2001;281(5):R1545–R1552. doi: 10.1152/ajpregu.2001.281.5.R1545
- Bönisch H, Fink KB, Malinowska B, et al. Serotonin and beyond — a tribute to Manfred Göthert (1939–2019). Naunyn Schmiedebergs Arch Pharmacol. 2021;394(9):1829–1867. doi: 10.1007/s00210-021-02083-5
- Bettikher OA, Belyaeva OA, Dukovich AI, et al. Expression of the serotonergic system components in the placenta in various types of preeclampsia. Journal of Obstetrics and Women’s Diseases. 2023;72(1):5–16. EDN: BFRVOI doi: 10.17816/JOWD110890
Supplementary files
