Email this article Development and testing of an endometrial status assessment test based on RNAampliSeq technology
- Authors: Malysheva O.V.1, Vashukova E.S.1, Gzgzyan A.M.1, Dzhemlikhanova L.K.1, Ob'edkova K.V.1, Popova A.K.1, Tkachenko A.A.1, Shalina M.A.1, Yarmolinskaya M.I.1, Glotov A.S.1
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Affiliations:
- The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
- Issue: Vol 72, No 6 (2023)
- Pages: 77-88
- Section: Original Research
- URL: https://journals.eco-vector.com/jowd/article/view/623969
- DOI: https://doi.org/10.17816/JOWD623969
- ID: 623969
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Abstract
BACKGROUND: Approximately two thirds of all implantation failures are due to impaired endometrial receptivity. The lack of implantation may be due to an implantation window failure found in approximately 25-30% of women with a history of failure to perform assisted reproductive technology protocols. The receptivity phase in such patients can be shifted in terms of timing, have a short duration, or not form. Currently, there are several commercial tests for determining endometrial receptivity based on transcriptomic data. However, these tests differ very significantly from each other in the set of genes studied, and the exact mechanism of endometrial receptivity is still not fully understood.
AIM: The aim of this study was to create and check our own test based on RNA AmpliSeq technology to assess the receptivity status of the endometrium and the implantation window.
MATERIALS AND METHODS: We previously created an RNAampliSeq panel containing 421 gens. With its use, the differential expression of these genes was analyzed in 38 endometrial samples taken in the proliferative and receptive phases.
RESULTS: The studied samples form clearly distinguishable clusters with the receptive and proliferative endometria. 271 genes from our panel are differentially expressed in different phases of the menstrual cycle.
CONCLUSIONS: We have created and tested a model that allows clearly distinguishing between the proliferative endometrium and the receptive one and identifying patients with disorders of the menstrual cycle.
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About the authors
Olga V. Malysheva
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: omal99@mail.ru
ORCID iD: 0000-0002-8626-5071
SPIN-code: 1740-2691
Cand. Sci. (Biol.)
Russian Federation, Saint PetersburgElena S. Vashukova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: vi_lena@list.ru
ORCID iD: 0000-0002-6996-8891
SPIN-code: 2811-8730
Cand. Sci. (Biol.)
Russian Federation, Saint PetersburgAlexander M. Gzgzyan
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: agzgzyan@gmail.com
ORCID iD: 0000-0003-3917-9493
SPIN-code: 6412-4801
MD, Dr. Sci. (Med.)
Russian Federation, Saint PetersburgLyailya Kh. Dzhemlikhanova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: dzhemlikhanova_l@mail.ru
ORCID iD: 0000-0001-6842-4430
SPIN-code: 1691-6559
MD, Cand. Sci. (Med.)
Russian Federation, Saint PetersburgKsenia V. Ob'edkova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: iagmail@ott.ru
ORCID iD: 0000-0002-2056-7907
SPIN-code: 2709-2890
MD, Cand. Sci. (Med.)
Russian Federation, Saint PetersburgAnastasiia K. Popova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: stassi1997@mail.ru
ORCID iD: 0009-0008-3512-2557
Russian Federation, Saint Petersburg
Alexander A. Tkachenko
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: castorfiber@list.ru
ORCID iD: 0000-0001-7985-0216
Russian Federation, Saint Petersburg
Maria A. Shalina
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Author for correspondence.
Email: amarus@inbox.ru
ORCID iD: 0000-0002-5921-3217
SPIN-code: 6673-2660
MD, Cand. Sci. (Med.)
Russian Federation, Saint PetersburgMaria I. Yarmolinskaya
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: m.yarmolinskaya@gmail.com
ORCID iD: 0000-0002-6551-4147
SPIN-code: 3686-3605
MD, Dr. Sci. (Med.), Professor of the Russian Academy of Sciences
Russian Federation, Saint PetersburgAndrey S. Glotov
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: anglotov@mail.ru
ORCID iD: 0000-0002-7465-4504
SPIN-code: 1406-0090
Dr. Sci. (Biol.)
Russian Federation, Saint PetersburgReferences
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