Role of vimentin in the morphogenesis of endometrial decidualization during pregnancy with IVF methods

Cover Page


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

BACKGROUND: Early reproductive loss is a complex problem, the solution of which requires a systematic approach to the study of the morphogenesis of gravid transformation. Violations of the morphofunctional characteristics of the endometrium are associated with chronic endometritis, which entails implantation pathology and the development of pregnancy, while formulating indications for the use of IVF methods. Chronic endometritis disrupts the differentiation of stromal cells and intermediate filaments with subsequent pathology of decidualization and gravid transformation.

AIM: The aim of this study was to evaluate vimentin expression in decidual cells of the gravid endometrium in an undeveloped first-trimester pregnancy after IVF, depending on its transformation in patients with chronic endometritis.

MATERIALS AND METHODS: Histological and immunohistochemical studies were performed on 40 samples of abortion material with normal chorionic villi karyotype from a non-developing pregnancy after IVF in patients with chronic endometritis and 15 samples of abortion material from a progressive pregnancy, surgically terminated at the woman’s request at similar terms. Two groups were formed — I group with a full-fledged gravid transformation in a non-developing pregnancy after IVF (n = 20) and II group with an incomplete gravid transformation in a non-developing pregnancy after IVF (n = 20). Histological examination was performed according to the standard procedure. Immunohistochemical study was performed using a one-step protocol with antigen unmasking. Primary monoclonal antibodies to vimentin (Clon V9, Diagnostic BioSystems Inc., Spain) were used at a 1 : 1000 dilution. Vimentin expression was evaluated in the compact layer of the gravid endometrium.

RESULTS: A full-fledged gravid transformation of the endometrial stroma in the abortion material in study group I and the control group was represented by mature deciduocytes of the epithelioid type, round or polygonal in shape, with a light vesicular nucleus. With an incomplete gravid transformation, the compact layer of the gravid endometrium was represented by fibroblasts with varying degrees of cytoplasmic development, from mild changes to the formation of pre-decidual and immature decidual cells. A decrease in vimentin expression was verified in the gravid endometrium after IVF compared to the control group. In case of an incomplete gravid transformation, a decrease in vimentin expression was also verified compared to the control group and study group I.

CONCLUSIONS: The presence of chronic endometritis in patients with infertility and chronic endometritis not only causes pathology of the original endometrium, but also disrupts decidualization, leading to reproductive loss with normal embryo karyotype.

Full Text

Restricted Access

About the authors

Tatiana G. Tral

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott

Email: ttg.tral@yandex.ru
ORCID iD: 0000-0001-8948-4811
SPIN-code: 1244-9631

MD, Cand. Sci. (Med.)

Russian Federation, Saint Petersburg

Gulrukhsor Kh. Tolibova

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott

Author for correspondence.
Email: gulyatolibova@yandex.ru
ORCID iD: 0000-0002-6216-6220
SPIN-code: 7544-4825

MD, Dr. Sci. (Med.)

Russian Federation, Saint Petersburg

References

  1. Tral TG, Tolibova GH. Morphological variants of gravidar transformation of the endometrium in non-developing pregnancy after in vitro fertilization. Clinical and Experimental Morphology. 2021;10(5):40–49. EDN: FTVABH doi: 10.31088/CEM2023.12.1.24-33
  2. Uryupina KV, Kutsenko II, Kravtsova EI, et al. Endometrial factor of infertility in patients of late reproductive age (review). Kuban Scientific Medical Bulletin. 2020;27(6):149–163. EDN: KMNINR doi: 10.25207/1608-6228-2020-27-6-149-163
  3. Leshchenko OYa. Chronic endometritis and reproductive disorders: versions and contraversions. Bulletin of Siberian medicine. 2020;19(3):166–176. EDN: LSARJA doi: 10.20538/1682-0363-2020-3-166-176
  4. Tral TG, Tolibova GH, Kogan IY. Endometrial implantation failure in cycles of in vitro fertilization in patients with chronic endometritis. Clinical and experimental morphology. 2023;12(1):24–33. EDN: HTDWJD doi: 10.31088/CEM2023.12.1.24-33
  5. Voropaeva EE, Kazachkov EL, Kazachkova EA. Chronic endometritis: clinical and morphological aspects of diagnosis. Chelyabinsk: Titul Publishing Center; 2022. (In Russ.) EDN: JFFYWZ
  6. Kitaya K, Takeuchi T, Mizuta S, et al. Endometritis: new time, new concepts. Fertil Steril. 2018;110(3):344–350. doi: 10.1016/j.fertnstert.2018.04.012
  7. Shaulov T, Sierra S, Sylvestre C. Recurrent implantation failure in IVF: a Canadian fertility and andrology society clinical practice guideline. Reprod Biomed Online. 2020;41(5).819–833. doi: 10.1016/j.rbmo.2020.08.007
  8. Ticconi C, Di Simone N, Campagnolo L, et al. Clinical consequences of defective decidualization. Tissue Cell. 2021;72. doi: 10.1016/j.tice.2021.101586
  9. Kimura F, Takebayashi A, Ishida M, et al. Review: chronic endometritis and its effect on reproduction. J Obstet Gynaecol Res. 2019;45(5):951–960. doi: 10.1111/jog.13937
  10. Puente E, Alonso L, Laganà AS, et al. Chronic endometritis: old problem, novel insights and future challenges. Int J Fertil Steril. 2020;13(4):250–256. doi: 10.22074/ijfs.2020.5779
  11. Wu D, Kimura F, Zheng L, et al. Chronic endometritis modifies decidualization in human endometrial stromal cells. Reprod Biol Endocrinol. 2017;15(1):16. doi: 10.1186/s12958-017-0233-x
  12. Chavan AR, Bhullar BA, Wagner GP. What was the ancestral function of decidual stromal cells? A model for the evolution of eutherian pregnancy. Placenta. 2016;40:40–51. doi: 10.1016/j.placenta.2016.02.012
  13. Ghosh J, Mainigi M, Coutifaris C, et al. Outlier DNA methylation levels as an indicator of environmental exposure and risk of undesirable birth outcome. Hum Mol Genet. 2016;25(1):123–129. doi: 10.1093/hmg/ddv458
  14. Ghosh J, Coutifaris C, Sapienza C, et al. Global DNA methylation levels are altered by modifiable clinical manipulations in assisted reproductive technologies. Clin Epigenetics. 2017;9:14. doi: 10.1186/s13148-017-0318-6
  15. Sultana S, Kajihara T, Mizuno Y, et al. Overexpression of microRNA-542-3p attenuates the differentiating capacity of endometriotic stromal cells. Reprod Med Biol. 2017;16(2):170–178. doi: 10.1002/rmb2.12028
  16. Kliman HJ, Frankfurter D. Clinical approach to recurrent implantation failure: evidence-based evaluation of the endometrium. Fertil Steril. 2019;111(4):618–628. doi: 10.1016/j.fertnstert.2019.02.011.
  17. Liang YX, Liu L, Jin ZY, et al. The high concentration of progesterone is harmful for endometrial receptivity and decidualization. Sci Rep. 2018;15(8):712. doi: 10.1038/s41598-017-18643-w
  18. Loschke F, Seltmann K, Bouameur JE, et al. Regulation of keratin network organization. Curr Opin Cell Biol. 2015;32:56–64. doi: 10.1016/j.ceb.2014.12.006
  19. Graubner FR, Tavares Pereira M, Boos A, et al. Canine decidualization in vitro: extracellular matrix modification, progesterone mediated effects and selective blocking of prostaglandin E2 receptors. J Reprod Dev. 2020;66(4):319–329. doi: 10.1262/jrd.2019-157
  20. Sharma P, Bolten ZT, Wagner DR, et al. Deformability of human mesenchymal stem cells is dependent on vimentin intermediate filaments. Ann Biomed Eng. 2017;45(5):1365–1374. doi: 10.1007/s10439-016-1787-z
  21. Jiu Y, Lehtimäki J, Tojkander S, et al. Bidirectional interplay between vimentin intermediate filaments and contractile actin stress fibers. Cell Rep. 2015;16(11):1511–1518. doi: 10.1016/j.celrep.2015.05.008
  22. Zhang XH, Liang X, Liang XH, et al. The mesenchymal-epithelial transition during in vitro decidualization. Reprod Sci. 2013;20(4):354–360. doi: 10.1177/1933719112472738
  23. Can A, Tekelioğlu M, Baltaci A. Expression of desmin and vimentin intermediate filaments in human decidual cells during first trimester pregnancy. Placenta. 1995;16(3):261–275. doi: 10.1016/0143-4004(95)90113-2
  24. Milovanov AP, Kuznetsova NB, Fokina TV. Role of immune distribution of tissue factor in the development of hemostasis during the first trimester of normal pregnancy. Bull Exp Biol Med. 2019;166(4):503–506. doi: 10.1007/s10517-019-04382-7
  25. Ridge KM, Eriksson JE, Pekny M, et al. Roles of vimentin in health and disease. Genes Dev. 2022;36(7–8):391–407. doi: 10.1101/gad.349358.122
  26. Maltseva LI, Sharipova RI, Zhelezova ME. Chronic endometritis — a change of habitual ideas. Practical medicine. 2018;(6):99–105. EDN: YBTOXZ doi: 10.32000/2072-1757-2018-16-6-99-105
  27. Orazov MR, Mikhaleva LM, Semenov PA Chronic endometritis: pathogenesis, diagnosis, management and associated infertility. Clinical and experimental morphology. 2020;9(2):16–25. EDN: WYWNBS doi: 10.31088/CEM2020.9.2.16-25
  28. Tolibova GH, Tral TG, Kogan IYu., et al. Endometrium: atlas. Moscow: StatusPraesens; 2022. EDN: GMDUEO
  29. Lucas ES, Dyer NP, Murakami K, et al. Loss of endometrial plasticity in recurrent pregnancy loss. Stem. Cells. 2016;34(2):346–3564. doi: 10.1002/stem.2222
  30. Mei J, Yan Y, Li SY. et al. CXCL16/CXCR6 interaction promotes endometrial Decidualization via the PI3K/AKT pathway. Reproduction. 2019;157(3):273–282. doi: 10.1530/REP-18-0417
  31. Saben JL, Asghar Z, Rhee JS, et al. Excess maternal fructose consumption increases fetal loss and impairs endometrial decidualization in mice. Endocrinology. 2016;157(2):956–968. doi: 10.1210/en.2015-1618
  32. Ochiai A, Kuroda K. Preconception resveratrol intake against infertility: friend or foe? Reprod Med Biol. 2020;19(2):107–113. doi: 10.1002/rmb2.12303
  33. Okada H, Tsuzuki T, Murata H. Decidualization of the human endometrium. Reprod Med Biol. 2018;17(3):220–227. doi: 10.1002/rmb2.12088
  34. Piva M, Flieger O, Rider V. Growth factor control of cultured rat uterine stromal cell proliferation is progesterone dependent. Biol Reprod. 1996;55(6):1333–1342. doi: 10.1095/biolreprod55.6.1333
  35. Chen J, Ren W, Lin L. Abnormal cGMP-dependent protein kinase I-mediated decidualization in preeclampsia. Hypertens Res. 2021;44(3):318–324. doi: 10.1038/s41440-020-00561-6
  36. Milovanov AP, Fokina TV, Rasstrigina IM. Inflammatory factor in the pathogenesis of undeveloped pregnancy. Scientific bulletin. 2013;(25):97–99. EDN: SIHELV

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Figure. Gravid endometrium in abortion material: a, with a full-fledged gravid transformation of the compact layer of the endometrium (control group), hematoxylin and eosin staining, zoom ×200; b, vimentin expression (control group), immunohistochemical study, zoom ×400; c, with a full-fledged gravid transformation of the compact layer of the endometrium (I group), hematoxylin and eosin staining, zoom ×200; d, vimentin expression (I group), immunohistochemical study, zoom ×400; e, with an incomplete gravid transformation of the compact layer of the endometrium (II group), hematoxylin and eosin staining, zoom ×200; f, vimentin expression (II group), immunohistochemical study, zoom ×400

Download (722KB)
Indexing metadata

Copyright (c) 2024 Eсо-Vector

License URL: https://eco-vector.com/for_authors.php#07
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 66759 от 08.08.2016 г. 
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия Эл № 77 - 6389 от 15.07.2002 г.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies