Prospects for using a proteomic approach to evaluate the effectiveness of implantation of embryos transferred in an IVF cycle

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This review presents non-invasive ways to evaluate the implantation potential of embryos that have entered the IVF cycle. A morphological approach to assessing the quality of embryos used in clinical practice is described. In addition, non-invasive approaches such as cell-free DNA analysis in culture medium, assessment of morphokinetic changes of individual embryos using artificial intelligence models, analysis of oxygen consumption by individual embryos, and the proteomic approach are considered in the literature as additions to the existing embryo quality assessment system. The focus of this article is on the identification of protein molecules in spent culture media of cleavage embryos as the most promising and poorly studied approach. The diagnostic significance of human chorionic gonadotropin and its isoforms, tumor necrosis factor alpha, interleukin-6, -8, -1β, human leukocyte antigens-G, stem cell factor, interferon-α, chemokines (CCL15, CCL27, CXCL12), platelet endothelial cell adhesion molecule 1, tissue inhibitors of metalloproteinase 4, E-selectin, von Willebrand factor and other compounds as biomarkers of embryo implantation potential is described. The analysis of these proteins in the spent culture media of embryos can complement the morphological assessment of embryo quality when making decisions on choosing an embryo for transfer.

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作者简介

Maria Zementova

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott

编辑信件的主要联系方式.
Email: marizementova@mail.ru
ORCID iD: 0000-0001-5161-369X
俄罗斯联邦, Saint Petersburg

Sergey Selkov

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott

Email: selkovsa@mail.ru
ORCID iD: 0000-0003-1560-7529
SPIN 代码: 7665-0594

MD, Dr. Sci. (Medicine), Professor, Honored Scientist of the Russian Federation

俄罗斯联邦, Saint Petersburg

Dmitry Dmitry

The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott

Email: falcojugger@yandex.ru
ORCID iD: 0000-0002-5749-2531
SPIN 代码: 3746-0000

Dr. Sci. (Biology)

俄罗斯联邦, Saint Petersburg

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2. Fig. 1. Stages of human implantation

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3. Fig. 2. Search for correlation of proteomic markers levels with implantation efficiency. IVF ICSI — in vitro fertilization and intracytoplasmic sperm injection

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