Kisspeptin and polycystic ovary syndrome - is there any connection?

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Abstract


Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women of reproductive age. It is known that kisspeptin stimulates activity of GnRH neurons and secretion of FSH and LH, thus disruption of interaction between kisspeptin and its receptor leads to anovulation. The aim of the study was to investigate the role kisspeptin in the pathogenesis of polycystic ovary syndrome. Materials and methods. The study included 14 patients with classic phenotype of PCOS and 11 healthy women of the control group. All the patients underwent laparoscopy and hysteroscopy with histological examination of ovarian tissue and endometrium. Determination of kisspeptin, FSH, LH, prolactin, AMH, estradiol, estrone, androgens (free testosterone and dehydroepiandrosterone sulfate) levels in peripheral blood in healthy women and patients with PCOS was performed by ELISA on the 2d and 8th days of menstrual cycle. Progesterone levels were investigated on the 18th-22d days of menstrual cycle. Expression of kisspeptin and its receptor in ovarian tissue and endometrium was estimated using immunohistochemical method. Results. Level of kisspeptin in peripheral blood of patients with PCOS tends to increase compared to its level in the control group, but the found difference was not reliable. Direct correlation between serum level of kisspeptin and levels of LH, free testosterone and DHEA-S was revealed in patients with PCOS. Immunohistochemical study in patients with PCOS showed a significant increase in the area of expression of КІЅЅ1 and KISS1R receptor in endometrium and in ovarian biopsies compared to these values in the control group. Conclusion. The obtained data show a definite role of kisspeptin in pathogenesis of polycystic ovary syndrome, but further research is needed.


Maria I. Yarmolinskaya

Author for correspondence.
m.yarmolinskaya@gmail.com
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, Saint Petersburg

professor of RAS, head of department of endocrinology of reproduction, Doctor of medical sciences, head of center “Diagnostics and treatment of endometriosis”

Nigar F. Ganbarli

n-ganbarli@mail.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott Saint Petersburg

3rd year post-graduate student of Operative gynecology department

Natalia N. Tkachenko

liberin@mail.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, Saint Petersburg

PhD, Head of the hormonal laboratory

Vera I. Nikolaeva

vin-161953@mail.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott Saint Petersburg

laboratoty assistant researcher of hormonal laboratory

Gulrukhsor Kh. Tolibova

gulyatolibova@mail.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, Saint Petersburg

PhD, MD

Tatyana G. Tral

TTG2008@bk.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, Saint Petersburg

MD, Pathologist. Head of Pathologist Laboratory, Department of Pathomorphology

Viktor V. Rulev

vrulev@mail.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, Saint Petersburg

Candidate of medical sciences, Researcher Endocrinology of Reproduction department

Anna A. Tsypurdeeva

tsypurdeeva@mail.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, Saint Petersburg

Candidate of Medical Sciences, Head of Operative gynecology department

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