Ovarian aromatase activity: evaluation methods and the clinical significance in IVF protocols

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Abstract


Introduction. Cytochrome P450 aromatase is a key enzyme in the biosynthesis of estrogens from androgens. Some gynecological diseases are accompanied with changes in the activity of ovarian aromatase. Moreover, changes in the acti vity of ovarian aromatase are associated with the response to standard gonadotrophin stimulation in women undergoing in vitro fertilization. A new effective method determining the activity of ovarian aromatase without using ovarian tissue biopsy has been developed.

The purpose of the present study was to compare the detection of ovarian aromatase activity using the ratio of estradiol to AMH on the second day of the menstrual cycle through the immunocytochemical evaluation of aromatase expression in cumulus cells.

Methods. Depending on the activity of the ovarian aromatase, calculated on the second day of the menstrual cycle, 16 patients undergoing IVF protocols were categorized into three groups: eight patients with low activity, four with normal activity, and four with high activity. The patients did not differ in age and BMI. The expression of aromatase in cumulus cells obtained through transvaginal puncture was investigated by the immunocytochemical method.

Results. The fluorescence intensities in the low, normal, and high activity groups were 134 (122–161), 238 (201–251), and 259 (240–299), respectively. Differences between these groups were statistically significant (p = 0.008). There was a pronounced positive correlation between the activity of ovarian aromatase, calculated on day two of the menstrual cycle, and the intensity of aromatase fluorescence in cumulus cells (Spearman, r = 0.75, p = 0.001, n = 16).


Pavel P. Yakovlev

Author for correspondence.
iakovlevpp@gmail.com
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, St. Petersburg

PhD Student

Julia S. Krylova

emerald2008@mail.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, St. Petersburg

PhD, MD, Scientist. Laboratory of Cell Biology, Department of Pathomorphology

Irina D. Mekina

rolerus@mail.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, St. Petersburg

Candidate of Biological Sciences. Research Fellow of Department Reproductive Technologies

Victoria O. Polyakova

vopol@yandex.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, St. Petersburg

Professor, Dr. Sc., Head of Laboratory of Cell Biology, Department of Pathomorphology

Igor M. Kvetnoy

igor.kvetnoy@yandex.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, St. Petersburg

MD, Professor. Head of the Pathomorphology Department

Marina A. Tarasova

tarasova@ott.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, St. Petersburg

MD, PhD, Professor

Igor Yu. Kogan

ikogan@mail.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, St. Petersburg

PhD, AM RAM, Scientific Secretary

Alexander M. Gzgzyan

iagmail@ott.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, St. Petersburg

MD, Head of Department Reproductive Technologies

Valeriia R. Rodichkina

rolerus@mail.ru
Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Russian Federation, St. Petersburg, Russia

Junior Research Scientist of the Pathomorphology Department

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Supplementary files

Supplementary Files Action
1. Fig. 1. Immunocytochemistry, × 400, the aromatase expression in cumulus cells (red fluorescence): a — high intensity; b — normal intensity; c — low intensity View (343KB) Indexing metadata
2. Fig. 2. The dependence between the coefficient of the ovarian aromatase activity and the expression of the aromatase P450 in cumulus cells View (55KB) Indexing metadata

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Copyright (c) 2018 Yakovlev P.P., Krylova J.S., Mekina I.D., Polyakova V.O., Kvetnoy I.M., Tarasova M.A., Kogan I.Y., Gzgzyan A.M., Rodichkina V.R.

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