Email this article Follicular fluid lipid profile assessment using MALDI mass spectrometry
- Authors: Mahmadalieva M.R.1, Ryzhov J.R.1, Gladchuk A.S.2,3, Podolskaya E.P.3, Shustov E.B.2, Lesik E.A.1, Komarova E.M.1, Ishchuk M.A.1, Sagurova Y.M.1, Tapilskaya N.I.1, Gzgzyan A.M.1, Kogan I.Y.1
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Affiliations:
- The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
- Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency
- Institute for Analytical Instrumentation RAS
- Issue: Vol 74, No 4 (2025)
- Pages: 45-54
- Section: Original study articles
- Submitted: 16.06.2025
- Accepted: 18.08.2025
- Published: 27.08.2025
- URL: https://journals.eco-vector.com/jowd/article/view/683396
- DOI: https://doi.org/10.17816/JOWD683396
- EDN: https://elibrary.ru/NTUBKE
- ID: 683396
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Abstract
BACKGROUND: Fatty acids are important components of the oocyte microenvironment, exhibiting both pro-inflammatory and lipotoxic effects as well as anti-inflammatory effects depending on the presence and quantity of unsaturated bonds. Changes in the lipid profile of follicular fluid in obese patients may be a mechanism that leads to a decrease in oocyte competence and pregnancy rate in assisted reproductive technology programs. However, the available literature data is fragmentary and contradictory, which dictates the need for further research.
AIM: The aim of this study was to investigate the lipid profile of follicular fluid in patients undergoing assisted reproductive technology programs with ovarian stimulation depending on the body mass index using MALDI (matrix-assisted laser desorption/ionization) mass spectrometry.
METHODS: This study involved patients undergoing infertility treatment using assisted reproductive technology in a short protocol using gonadotropin-releasing hormone antagonists. The levels of essential saturated and unsaturated fatty acids were analyzed in the follicular fluid of the first aspirated follicle using MALDI mass spectrometry.
RESULTS: Two study groups were formed out of 138 patients: patients with normal body mass index (n = 38) and patients with overweight and obesity (n = 76). The latter were characterized by higher levels of myristic and stearic acids and lower levels of oleic acid in the follicular fluid. The level of myristic acid in the follicular fluid positively correlated with the body mass index, while the level of oleic acid negatively correlated with this parameter.
CONCLUSION: The data obtained indicate that overweight and obesity are associated with increased levels of saturated myristic and stearic acids in the follicular fluid, which negatively impact folliculogenesis, as well as a decrease in the level of monounsaturated oleic acid in the follicular fluid, which is able to compensate for the lipotoxic effects of saturated fatty acids.
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About the authors
Manizha R. Mahmadalieva
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: 2563737@mail.ru
ORCID iD: 0000-0002-9578-8931
SPIN-code: 1523-0196
Russian Federation, Saint Petersburg
Julian R. Ryzhov
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Author for correspondence.
Email: julian.ryzhov@gmail.com
ORCID iD: 0000-0002-5073-8279
SPIN-code: 8320-1234
Russian Federation, Saint Petersburg
Alexey S. Gladchuk
Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency; Institute for Analytical Instrumentation RAS
Email: aleglad24@gmail.com
ORCID iD: 0000-0002-4411-2069
SPIN-code: 1117-4865
Cand. Sci. (Engineering)
Russian Federation, Saint Petersburg; Saint PetersburgEkaterina P. Podolskaya
Institute for Analytical Instrumentation RAS
Email: ek.podolskaya@gmail.com
Dr. Sci. (Engineering)
Russian Federation, Saint PetersburgEvgeny B. Shustov
Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency
Email: shustov-msk@mail.ru
ORCID iD: 0000-0001-5895-688X
SPIN-code: 9665-6670
MD, Dr. Sci. (Medicine), Professor
Russian Federation, Saint PetersburgElena A. Lesik
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: lesike@yandex.ru
ORCID iD: 0000-0003-1611-6318
SPIN-code: 6102-4690
Cand. Sci. (Biology)
Russian Federation, Saint PetersburgEvgeniia M. Komarova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: evgmkomarova@gmail.com
ORCID iD: 0000-0002-9988-9879
SPIN-code: 1056-7821
Cand. Sci. (Biology)
Russian Federation, Saint PetersburgMariia A. Ishchuk
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: mashamazilina@gmail.com
ORCID iD: 0000-0002-4443-4287
SPIN-code: 1237-6373
Russian Federation, Saint Petersburg
Yanina M. Sagurova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: yanina.sagurova96@mail.ru
ORCID iD: 0000-0003-4947-8171
SPIN-code: 8908-7033
Russian Federation, Saint Petersburg
Natalia I. Tapilskaya
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: tapnatalia@yandex.ru
ORCID iD: 0000-0001-5309-0087
SPIN-code: 3605-0413
MD, Dr. Sci. (Medicine), Professor
Russian Federation, Saint PetersburgAlexander M. Gzgzyan
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: agzgzyan@gmail.com
ORCID iD: 0000-0003-3917-9493
SPIN-code: 6412-4801
MD, Dr. Sci. (Medicine), Professor
Russian Federation, Saint PetersburgIgor Yu. Kogan
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: ikogan@mail.ru
ORCID iD: 0000-0002-7351-6900
SPIN-code: 6572-6450
MD, Dr. Sci. (Medicine), Professor, Corresponding Member of the Russian Academy of Sciences
Russian Federation, Saint PetersburgReferences
- Gardner DK, Schoolcraft WB. Culture and transfer of human blastocysts. Curr Opin Obstet Gynecol. 1999;11(3):307–311. doi: 10.1097/00001703-199906000-00013
- Scheffer JB, Carvalho RF, Scheffer BB, et al. Correlations between clinical parameters, blastocyst morphological classification and embryo euploidy. JBRA Assist Reprod. 2024;28(1):54–58. EDN: GSWBGR doi: 10.5935/1518-0557.20230054
- Llonch S, Barragán M, Nieto P, et al. Single human oocyte transcriptome analysis reveals distinct maturation stage-dependent pathways impacted by age. Aging Cell. 2021;20(5):e13360. EDN: AFAVBS doi: 10.1111/acel.13360
- Ryzhov JR, Tkachenko NN, Mahmadalieva MR, et al. The follicular levels of adipokines and their ratio as the prognostic markers of in vitro fertilization outcomes. Gynecol Endocrinol. 2021;37(S1):31–34. EDN: YLXKLY doi: 10.1080/09513590.2021.2006512
- Komarova EM, Lesik EA, Ob’edkova KV, et al. Prognostic significance of cumulus cells mitochondrial dysfunction markers in obtaining optimal quality embryos in assisted reproductive technologies protocols. Russian Journal of Human Reproduction. 2023;29(6):57–64. EDN: SYLXBL doi: 10.17116/repro20232906157
- Kurowska P, Wyroba J, Pich K, et al. New aspect on the regulation of in vitro oocyte maturation: role of the obesity, neuropeptides and adipokines. J Assist Reprod Genet. 2024. EDN: FNQISN doi: 10.1007/s10815-024-03345-w
- Podolskaya EP, Gladchuk AS, Keltsieva OA, et al. Thin film chemical deposition techniques as a tool for fingerprinting of free fatty acids by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem. 2019;91(2):1636–1643. EDN: YRBJQX doi: 10.1021/acs.analchem.8b05296
- Kobayashi H, Imanaka S. Recent progress in metabolomics for analyzing common infertility conditions that affect ovarian function. Reprod Med Biol. 2024;23(1):e12609. EDN: GAMLST doi: 10.1002/rmb2.12609
- Pantasri T, Wu LL, Hull ML, et al. Distinct localisation of lipids in the ovarian follicular environment. Reprod Fertil Dev. 2015;27(4):593–601. doi: 10.1071/RD14321
- Valckx SD, Arias-Alvarez M, De Pauw I, et al. Fatty acid composition of the follicular fluid of normal weight, overweight and obese women undergoing assisted reproductive treatment: a descriptive cross-sectional study. Reprod Biol Endocrinol. 2014;12:13. EDN: GCGQUB doi: 10.1186/1477-7827-12-13
- Jungheim ES, Macones GA, Odem RR, et al. Associations between free fatty acids, cumulus oocyte complex morphology and ovarian function during in vitro fertilization. Fertil Steril. 2011;95(6):1970–1974. doi: 10.1016/j.fertnstert.2011.01.154
- Mirabi P, Chaichi MJ, Esmaeilzadeh S. Does different BMI influence oocyte and embryo quality by inducing fatty acid in follicular fluid? Taiwan J Obstet Gynecol. 2017;56(2):159–164. doi: 10.1016/j.tjog.2016.11.005
- Tanriover C, Copur S, Gaipov A, et al. Metabolically healthy obesity: Misleading phrase or healthy phenotype? Eur J Intern Med. 2023;111:5–20. EDN: HLWBZT doi: 10.1016/j.ejim.2023.02.025
- Giroli MG, Werba JP, Risé P, et al. Effects of Mediterranean diet or low-fat diet on blood fatty acids in patients with coronary heart disease. a randomized intervention study. Nutrients. 2021;13(7):2389. EDN: AAHYPR doi: 10.3390/nu13072389
- Köhler A, Bittner D, Löw A, et al. Effects of a convenience drink fortified with n-3 fatty acids on the n-3 index. Br J Nutr. 2010;104(5):729–736. doi: 10.1017/S0007114510001054
- Engel KM, Prabutzki P, Leopold J, et al. A new update of MALDI-TOF mass spectrometry in lipid research. Prog Lipid Res. 2022;86:101145. EDN: YHESUD doi: 10.1016/j.plipres.2021.101145
- Mu YM, Yanase T, Nishi Y, et al. Saturated FFAs, palmitic acid and stearic acid, induce apoptosis in human granulosa cells. Endocrinology. 2001;142(8):3590–3597. doi: 10.1210/endo.142.8.8293
- Zeng X, Zhu M, Liu X, et al. Oleic acid ameliorates palmitic acid induced hepatocellular lipotoxicity by inhibition of ER stress and pyroptosis. Nutr Metab (Lond). 2020;17:11. EDN: YSBOOV doi: 10.1186/s12986-020-0434-8
- Tremblay PG, Fortin C, Sirard MA. Gene cascade analysis in human granulosa tumor cells (KGN) following exposure to high levels of free fatty acids and insulin. J Ovarian Res. 2021;14(1):178. EDN: MRZWMR doi: 10.1186/s13048-021-00934-6
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