电邮这篇文章 Follicular fluid lipid profile assessment using MALDI mass spectrometry
- 作者: Mahmadalieva M.R.1, Ryzhov J.R.1, Gladchuk A.S.2,3, Podolskaya E.P.3, Shustov E.B.2, Lesik E.A.1, Komarova E.M.1, Ishchuk M.A.1, Sagurova Y.M.1, Tapilskaya N.I.1, Gzgzyan A.M.1, Kogan I.Y.1
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隶属关系:
- The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
- Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency
- Institute for Analytical Instrumentation RAS
- 期: 卷 74, 编号 4 (2025)
- 页面: 45-54
- 栏目: Original study articles
- ##submission.dateSubmitted##: 16.06.2025
- ##submission.dateAccepted##: 18.08.2025
- ##submission.datePublished##: 27.08.2025
- URL: https://journals.eco-vector.com/jowd/article/view/683396
- DOI: https://doi.org/10.17816/JOWD683396
- EDN: https://elibrary.ru/NTUBKE
- ID: 683396
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BACKGROUND: Fatty acids are important components of the oocyte microenvironment, exhibiting both pro-inflammatory and lipotoxic effects as well as anti-inflammatory effects depending on the presence and quantity of unsaturated bonds. Changes in the lipid profile of follicular fluid in obese patients may be a mechanism that leads to a decrease in oocyte competence and pregnancy rate in assisted reproductive technology programs. However, the available literature data is fragmentary and contradictory, which dictates the need for further research.
AIM: The aim of this study was to investigate the lipid profile of follicular fluid in patients undergoing assisted reproductive technology programs with ovarian stimulation depending on the body mass index using MALDI (matrix-assisted laser desorption/ionization) mass spectrometry.
METHODS: This study involved patients undergoing infertility treatment using assisted reproductive technology in a short protocol using gonadotropin-releasing hormone antagonists. The levels of essential saturated and unsaturated fatty acids were analyzed in the follicular fluid of the first aspirated follicle using MALDI mass spectrometry.
RESULTS: Two study groups were formed out of 138 patients: patients with normal body mass index (n = 38) and patients with overweight and obesity (n = 76). The latter were characterized by higher levels of myristic and stearic acids and lower levels of oleic acid in the follicular fluid. The level of myristic acid in the follicular fluid positively correlated with the body mass index, while the level of oleic acid negatively correlated with this parameter.
CONCLUSION: The data obtained indicate that overweight and obesity are associated with increased levels of saturated myristic and stearic acids in the follicular fluid, which negatively impact folliculogenesis, as well as a decrease in the level of monounsaturated oleic acid in the follicular fluid, which is able to compensate for the lipotoxic effects of saturated fatty acids.
全文:
作者简介
Manizha Mahmadalieva
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: 2563737@mail.ru
ORCID iD: 0000-0002-9578-8931
SPIN 代码: 1523-0196
俄罗斯联邦, Saint Petersburg
Julian Ryzhov
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
编辑信件的主要联系方式.
Email: julian.ryzhov@gmail.com
ORCID iD: 0000-0002-5073-8279
SPIN 代码: 8320-1234
俄罗斯联邦, Saint Petersburg
Alexey Gladchuk
Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency; Institute for Analytical Instrumentation RAS
Email: aleglad24@gmail.com
ORCID iD: 0000-0002-4411-2069
SPIN 代码: 1117-4865
Cand. Sci. (Engineering)
俄罗斯联邦, Saint Petersburg; Saint PetersburgEkaterina Podolskaya
Institute for Analytical Instrumentation RAS
Email: ek.podolskaya@gmail.com
Dr. Sci. (Engineering)
俄罗斯联邦, Saint PetersburgEvgeny Shustov
Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency
Email: shustov-msk@mail.ru
ORCID iD: 0000-0001-5895-688X
SPIN 代码: 9665-6670
MD, Dr. Sci. (Medicine), Professor
俄罗斯联邦, Saint PetersburgElena Lesik
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: lesike@yandex.ru
ORCID iD: 0000-0003-1611-6318
SPIN 代码: 6102-4690
Cand. Sci. (Biology)
俄罗斯联邦, Saint PetersburgEvgeniia Komarova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: evgmkomarova@gmail.com
ORCID iD: 0000-0002-9988-9879
SPIN 代码: 1056-7821
Cand. Sci. (Biology)
俄罗斯联邦, Saint PetersburgMariia Ishchuk
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: mashamazilina@gmail.com
ORCID iD: 0000-0002-4443-4287
SPIN 代码: 1237-6373
俄罗斯联邦, Saint Petersburg
Yanina Sagurova
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: yanina.sagurova96@mail.ru
ORCID iD: 0000-0003-4947-8171
SPIN 代码: 8908-7033
俄罗斯联邦, Saint Petersburg
Natalia Tapilskaya
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: tapnatalia@yandex.ru
ORCID iD: 0000-0001-5309-0087
SPIN 代码: 3605-0413
MD, Dr. Sci. (Medicine), Professor
俄罗斯联邦, Saint PetersburgAlexander Gzgzyan
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: agzgzyan@gmail.com
ORCID iD: 0000-0003-3917-9493
SPIN 代码: 6412-4801
MD, Dr. Sci. (Medicine), Professor
俄罗斯联邦, Saint PetersburgIgor Kogan
The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O. Ott
Email: ikogan@mail.ru
ORCID iD: 0000-0002-7351-6900
SPIN 代码: 6572-6450
MD, Dr. Sci. (Medicine), Professor, Corresponding Member of the Russian Academy of Sciences
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