Surgical methods of retinal pigment epithelium transplantation in rabbits in experiment
- Authors: Neroeva N.V.1, Lagarkova M.A.2, Ilyukhin P.A.1, Izmailova N.S.1, Utkina O.A.1, Laktionov P.P.3, Chernonosova V.S.3, Kharitonov A.E.2
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Affiliations:
- Helmholtz National Medical Research Center of Eye Diseases
- Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
- Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences
- Issue: Vol 16, No 1 (2023)
- Pages: 59-67
- Section: Experimental trials
- URL: https://journals.eco-vector.com/ov/article/view/237101
- DOI: https://doi.org/10.17816/OV237101
- ID: 237101
Cite item
Abstract
The article is devoted to the study of surgical methods of retinal pigment epithelium transplantation in experiment on rabbits. The history of retinal pigment epithelium transplantation is briefly characterized in the study. Characteristics of the methods used are presented, their limitations, possibilities, and requirements for implementation in practice are highlighted. The results of transplantation of suspension of retinal pigment epithelium cells differentiated from human induced pluripotent stem cells (IPSC-RPE) without and with the use of platelet-rich blood plasma (PRP) are also considered. According to the results of the analysis we have developed modified methods of IPSC-RPE cells transplantation in the experiment on rabbits, which allow to reduce the number of postoperative complications, which provides the best therapeutic effect. Prospects for further studies are associated with a comparative analysis of the proposed techniques in terms of cell survival at different terms, anatomical integration and functional efficiency in the dynamics.
Keywords
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About the authors
Natalia V. Neroeva
Helmholtz National Medical Research Center of Eye Diseases
Email: nneroeva@gmail.com
ORCID iD: 0000-0003-1038-2746
MD, Cand. Sci. (Med.), ophthalmologist of the Department of Pathology of the Retina and Optic Nerve
Russian Federation, MoscowMaria A. Lagarkova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Email: lagar@rcpcm.org
ORCID iD: 0000-0001-9594-1134
Dr. Sci. (Biol.), member of Russian Academy of Sciences, director
Russian Federation, MoscowPavel A. Ilyukhin
Helmholtz National Medical Research Center of Eye Diseases
Email: paulilukhin@gmail.com
ORCID iD: 0000-0001-9552-6782
SPIN-code: 2407-5436
Cand. Sci. (Med.), research associate, Department of Pathology of the Retina and Optic Nerve
Russian Federation, MoscowNatalya S. Izmailova
Helmholtz National Medical Research Center of Eye Diseases
Email: nizm2013@mail.ru
SPIN-code: 1984-1519
Cand. Sci. (Med.), leading research associate, head of the Department of Pathological Anatomy and Histology
Russian Federation, MoscowOlga Alexandrovna Utkina
Helmholtz National Medical Research Center of Eye Diseases
Author for correspondence.
Email: olga_utkina17@mail.ru
ORCID iD: 0000-0001-8463-6337
SPIN-code: 2465-0604
postgraduate student of the Department of Pathology of the Retina and Optic Nerve, ophthalmologist
Russian Federation, MoscowPavel P. Laktionov
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences
Email: lakt@niboch.nsc.ru
SPIN-code: 4114-3170
Cand. Sci. (Biol.), head of the Laboratory of Molecular Medicine
Russian Federation, NovosibirskVera S. Chernonosova
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences
Email: vera_mal@niboch.nsc.ru
SPIN-code: 1168-1817
Cand. Sci. (Biol.), research associate of the Laboratory of Molecular Medicine
Russian Federation, NovosibirskAnatoly E. Kharitonov
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Email: kharitonov.ae@rcpcm.org
SPIN-code: 9585-9205
junior research associate of the Laboratory of Cell Biology
Russian Federation, MoscowReferences
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