Surgical methods of retinal pigment epithelium transplantation in rabbits in experiment
- Authors: Neroeva N.V.1, Lagarkova M.A.2, Ilyukhin P.A.1, Izmailova N.S.1, Utkina O.A.1, Laktionov P.P.3, Chernonosova V.S.3, Kharitonov A.E.2
-
Affiliations:
- Helmholtz National Medical Research Center of Eye Diseases
- Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
- Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences
- Issue: Vol 16, No 1 (2023)
- Pages: 59-67
- Section: Experimental trials
- Submitted: 15.02.2023
- Accepted: 20.03.2023
- Published: 09.04.2023
- URL: https://journals.eco-vector.com/ov/article/view/237101
- DOI: https://doi.org/10.17816/OV237101
- ID: 237101
Cite item
Abstract
The article is devoted to the study of surgical methods of retinal pigment epithelium transplantation in experiment on rabbits. The history of retinal pigment epithelium transplantation is briefly characterized in the study. Characteristics of the methods used are presented, their limitations, possibilities, and requirements for implementation in practice are highlighted. The results of transplantation of suspension of retinal pigment epithelium cells differentiated from human induced pluripotent stem cells (IPSC-RPE) without and with the use of platelet-rich blood plasma (PRP) are also considered. According to the results of the analysis we have developed modified methods of IPSC-RPE cells transplantation in the experiment on rabbits, which allow to reduce the number of postoperative complications, which provides the best therapeutic effect. Prospects for further studies are associated with a comparative analysis of the proposed techniques in terms of cell survival at different terms, anatomical integration and functional efficiency in the dynamics.
Keywords
Full Text
![Restricted Access](https://journals.eco-vector.com/lib/pkp/templates/images/icons/text_lock.png)
About the authors
Natalia V. Neroeva
Helmholtz National Medical Research Center of Eye Diseases
Email: nneroeva@gmail.com
ORCID iD: 0000-0003-1038-2746
MD, Cand. Sci. (Med.), ophthalmologist of the Department of Pathology of the Retina and Optic Nerve
Russian Federation, MoscowMaria A. Lagarkova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Email: lagar@rcpcm.org
ORCID iD: 0000-0001-9594-1134
Dr. Sci. (Biol.), member of Russian Academy of Sciences, director
Russian Federation, MoscowPavel A. Ilyukhin
Helmholtz National Medical Research Center of Eye Diseases
Email: paulilukhin@gmail.com
ORCID iD: 0000-0001-9552-6782
SPIN-code: 2407-5436
Cand. Sci. (Med.), research associate, Department of Pathology of the Retina and Optic Nerve
Russian Federation, MoscowNatalya S. Izmailova
Helmholtz National Medical Research Center of Eye Diseases
Email: nizm2013@mail.ru
SPIN-code: 1984-1519
Cand. Sci. (Med.), leading research associate, head of the Department of Pathological Anatomy and Histology
Russian Federation, MoscowOlga Alexandrovna Utkina
Helmholtz National Medical Research Center of Eye Diseases
Author for correspondence.
Email: olga_utkina17@mail.ru
ORCID iD: 0000-0001-8463-6337
SPIN-code: 2465-0604
postgraduate student of the Department of Pathology of the Retina and Optic Nerve, ophthalmologist
Russian Federation, MoscowPavel P. Laktionov
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences
Email: lakt@niboch.nsc.ru
SPIN-code: 4114-3170
Cand. Sci. (Biol.), head of the Laboratory of Molecular Medicine
Russian Federation, NovosibirskVera S. Chernonosova
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences
Email: vera_mal@niboch.nsc.ru
SPIN-code: 1168-1817
Cand. Sci. (Biol.), research associate of the Laboratory of Molecular Medicine
Russian Federation, NovosibirskAnatoly E. Kharitonov
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Email: kharitonov.ae@rcpcm.org
SPIN-code: 9585-9205
junior research associate of the Laboratory of Cell Biology
Russian Federation, MoscowReferences
- Singh MS, Park SS, Albini TA, et al. Retinal stem cell transplantation: Balancing safety and potential. Prog Retin Eye Res. 2020;75:100779. doi: 10.1016/j.preteyeres.2019.100779
- Wong WL, Su X, Li X, et al. Global prevalence of age-related macular degeneration and disease burden projection for 2020 and 2040: a systematic review and meta-analysis. Lancet Glob Health. 2014;2(2): e106–e116. doi: 10.1016/S2214-109X(13)70145-1
- Verbakel SK, van Huet RAC, Boon CJF, et al. Non-syndromic retinitis pigmentosa. Prog Retin Eye Res. 2018;66:157–186. doi: 10.1016/j.preteyeres.2018.03.005
- Seah I, Liu Z, Wong DSL, et al. Retinal pigment epithelium transplantation in a non-human primate model for degenerative retinal diseases. J Vis Exp. 2021;172: e62638. doi: 10.3791/62638
- Gouras P, Flood MT, Kjeldbye H. Transplantation of cultured human retinal cells to monkey retina. An Acad Bras Cienc. 1984;56(4):431–443.
- Koster C, Wever KE, Wagstaff PE, et al. A systematic review on transplantation studies of the retinal pigment epithelium in animal models. Int J Mol Sci. 2020;21(8):2719. doi: 10.3390/ijms21082719
- Stanzel B, Ader M, Liu Z, et al. Surgical Approaches for Cell Therapeutics Delivery to the Retinal Pigment Epithelium and Retina. In: Bharti K, editor. Pluripotent Stem Cells in Eye Disease Therapy. Advances in Experimental Medicine and Biology. Vol. 1186. Springer, Cham, 2019. P. 141–170. doi: 10.1007/978-3-030-28471-8_6
- Kamao H, Mandai M, Okamoto S, et al. Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application. Stem Cell Rep. 2014;2(2):205–218. doi: 10.1016/j.stemcr.2013.12.007
- M’Barek KB, Bertin S, Brazhnikova E, et al. Clinical-grade production and safe delivery of human ESC derived RPE sheets in primates and rodents. Biomaterials. 2020;230:119603. doi: 10.1016/j.biomaterials.2019.119603
- Fujii S, Sugita S, Futatsugi Y, et al. A Strategy for Personalized Treatment of iPS-Retinal Immune Rejections Assessed in Cynomolgus Monkey Models. Int J Mol Sci. 2020;21(9):3077. doi: 10.3390/ijms21093077
- Kharitonov AE, Surdina AV, Lebedeva OS, et al. Possibilities for Using Pluripotent Stem Cells for Restoring Damaged Eye Retinal Pigment Epithelium. Acta Naturae. 2018;10(3):34–38. (In Russ.) doi: 10.32607/20758251-2018-10-3-30-39
- Maeda T, Sugita S, Kurimoto Y, Takahashi M. Trends of stem cell therapies in age-related macular degeneration. J Clin Med. 2021;10(8):1785. doi: 10.3390/jcm10081785
- Patent RUS № 2729937/13.08.2020. Byul. No. 23. Neroeva NV, Neroev VV, Katargina LA, et al. Sposob subretinalnoi transplantatsii kletok retinalnogo pigmentnogo ehpiteliya (RPEH), differentsirovannykh iz indutsirovannykh plyuripotentnykh stvolovykh kletok cheloveka pri atrofii retinalnogo pigmentnogo ehpiteliya v ehksperimente. Available at: https://i.moscow/patents/ru2729937c1_20200813 (In Russ.)
- Patent RUS № 2730937/26.08.2020. Byul. No. 24. Neroeva NV, Neroev VV, Katargina LA, et al. Method of transplantation of retinal pigment epithelium (rpe) cells differentiated from induced human pluripotent stem cells, with retinal pigment epithelium atrophy. Available at: https://patents.google.com/patent/RU2730937C1/ru (In Russ.)
- Neroeva NV, Neroev VV, Katargina LA, et al. Experimental stem cell replacement transplantation in retinal pigment epithelium atrophy. The Russian Annals of Ophthalmology. 2022;138(3):7–15. (In Russ.) doi: 10.17116/oftalma20221380317
- Gostev AA, Shundrina IK, Pastukhov VI, et al. Correction: Gostev et al. In Vivo Stability of Polyurethane-Based Electrospun Vascular Grafts in Terms of Chemistry and Mechanics. Polymers 2020, 12, 845. Polymers (Basel). 2022;14(11):2263. doi: 10.3390/polym14112263
- Gostev AA, Chernonosova VS, Murashov IS, et al. Electrospun polyurethane-based vascular grafts: physicochemical properties and functioning in vivo. Biomed Mater. 2020;15:015010. doi: 10.1088/1748–605X/ab550c
- Petrus-Reurer S, Bartuma H, Aronsson M, et al. Integration of Subretinal Suspension Transplants of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells in a Large-Eyed Model of Geographic Atrophy. Invest Ophthalmol Vis Sci. 2017;58(2): 1314–1322. doi: 10.1167/iovs.16-20738
- Brant Fernandes RA, Stefanini FR, Falabella P, et al. Development of a new tissue injector for subretinal transplantation of human embryonic stem cell derived retinal pigmented epithelium. Int J RetinVitr. 2017;3:41. doi: 10.1186/s40942-017-0095-6
- Lu B, Malcuit C, Wang S, et al. Long-term safety and function of RPE from human embryonic stem cells in preclinical mo dels of macular degeneration. Stem Cells. 2009;27(9):2126–2135. doi: 10.1002/stem.149
- Shkvorchenko DO, Zakharov VD, Shpak AA, et al. Nash opyt primeneniya bogatoi trombotsitami plazmy krovi v khirurgii makulyarnykh razryvov. Modern technologies in ophthalmology. 2016;9(1):245–246. (In Russ.)
Supplementary files
![](/img/style/loading.gif)