Surgical methods of retinal pigment epithelium transplantation in rabbits in experiment

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Abstract

The article is devoted to the study of surgical methods of retinal pigment epithelium transplantation in experiment on rabbits. The history of retinal pigment epithelium transplantation is briefly characterized in the study. Characteristics of the methods used are presented, their limitations, possibilities, and requirements for implementation in practice are highlighted. The results of transplantation of suspension of retinal pigment epithelium cells differentiated from human induced pluripotent stem cells (IPSC-RPE) without and with the use of platelet-rich blood plasma (PRP) are also considered. According to the results of the analysis we have developed modified methods of IPSC-RPE cells transplantation in the experiment on rabbits, which allow to reduce the number of postoperative complications, which provides the best therapeutic effect. Prospects for further studies are associated with a comparative analysis of the proposed techniques in terms of cell survival at different terms, anatomical integration and functional efficiency in the dynamics.

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About the authors

Natalia V. Neroeva

Helmholtz National Medical Research Center of Eye Diseases

Email: nneroeva@gmail.com
ORCID iD: 0000-0003-1038-2746

MD, Cand. Sci. (Med.), ophthalmologist of the Department of Pathology of the Retina and Optic Nerve

Russian Federation, Moscow

Maria A. Lagarkova

Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Email: lagar@rcpcm.org
ORCID iD: 0000-0001-9594-1134

Dr. Sci. (Biol.), member of Russian Academy of Sciences, director

Russian Federation, Moscow

Pavel A. Ilyukhin

Helmholtz National Medical Research Center of Eye Diseases

Email: paulilukhin@gmail.com
ORCID iD: 0000-0001-9552-6782
SPIN-code: 2407-5436

Cand. Sci. (Med.), research associate, Department of Pathology of the Retina and Optic Nerve

Russian Federation, Moscow

Natalya S. Izmailova

Helmholtz National Medical Research Center of Eye Diseases

Email: nizm2013@mail.ru
SPIN-code: 1984-1519

Cand. Sci. (Med.), leading research associate, head of the Department of Pathological Anatomy and Histology

Russian Federation, Moscow

Olga Alexandrovna Utkina

Helmholtz National Medical Research Center of Eye Diseases

Author for correspondence.
Email: olga_utkina17@mail.ru
ORCID iD: 0000-0001-8463-6337
SPIN-code: 2465-0604

postgraduate student of the Department of Pathology of the Retina and Optic Nerve, ophthalmologist

Russian Federation, Moscow

Pavel P. Laktionov

Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences

Email: lakt@niboch.nsc.ru
SPIN-code: 4114-3170

Cand. Sci. (Biol.), head of the Laboratory of Molecular Medicine

Russian Federation, Novosibirsk

Vera S. Chernonosova

Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences

Email: vera_mal@niboch.nsc.ru
SPIN-code: 1168-1817

Cand. Sci. (Biol.), research associate of the Laboratory of Molecular Medicine

Russian Federation, Novosibirsk

Anatoly E. Kharitonov

Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Email: kharitonov.ae@rcpcm.org
SPIN-code: 9585-9205

junior research associate of the Laboratory of Cell Biology

Russian Federation, Moscow

References

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Supplementary files

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2. Fig. 1. Results of transplantation of a suspension of retinal pigment epithelium cells differentiated from human induced pluripotent stem cells (IPSC-RPE) ) without and with the use of platelet-rich blood plasma (PRP)

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3. Fig. 2. OCT image of rabbit retina after substrate transplantation with retinal pigment epithelium cells differentiated from human induced pluripotent stem cells

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4. Fig. 3. Optical coherenc tomogram of the rabbit retina after transplantation of a prepared round-shaped substrate with retinal pigment epithelium cells differentiated from human induced pluripotent stem cells

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5. Fig. 4. Results of cell transplantation on the substrate without and with modification

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6. Fig. 5. Preparation of retinal pigment epithelium cells differentiated from human induced pluripotent stem cells bolus injection area. 1 — monolayer-like cells; 2 — retina; 3 —vasculature. Staining — hematoxylin and eosin, magnification ×200, in inset ×630

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7. Fig. 6. Implanted artificial membrane (indicated by arrow) at late term. Hematoxylin and eosin staining, magnification ×100

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