Evaluation of Effectiveness of New Samples of Chitosan-Based Local Hemostatic Agents After Liver Resection in Experiment
- 作者: Lipatov V.A.1, Fronchek E.V.2, Grigor'yan A.Y.1, Severinov D.A.1, Naimzada M.1, Zakutayeva L.Y.1
-
隶属关系:
- Kursk State Medical University
- Evers Group Rus
- 期: 卷 31, 编号 1 (2023)
- 页面: 89-96
- 栏目: Original study
- ##submission.dateSubmitted##: 21.05.2022
- ##submission.dateAccepted##: 29.08.2022
- ##submission.datePublished##: 03.04.2023
- URL: https://journals.eco-vector.com/pavlovj/article/view/108094
- DOI: https://doi.org/10.17816/PAVLOVJ108094
- ID: 108094
如何引用文章
详细
INTRODUCTION: Chitosan-based local hemostatic agents are most promising in terms of effective stoppage of bleeding, additional properties (for example, antibacterial effect) and stimulation of regeneration. New forms of them are being developed for different types of organ damage.
AIM: To evaluate the hemostatic effect of samples of new chitosan-based local hemostatic agents on a liver resection model.
MATERIALS AND METHODS: An in vivo experiment was performed on 60 white male rats of Wistar line of 200 g–250 g mass. The animals were divided to 4 study groups of 15 animals, respectively, depending on the kind of hemostatic agent and additional introduction of an anticoagulant that enhanced bleeding. As study materials, hemostatic collagen sponge (control groups No. 1.1 and 1.2) and also samples of new chitosan-based hemostatic agents — Сhitocol-Hemo® (Evers, Russia) were used. The rats under general anesthesia underwent midline laparotomy followed by laparopexy by dissecting the falciform ligament of the liver and placement of a gauze turunda between the diaphragm and the left liver lobule with displacement of the latter into the wound. After this, a sterile gauze turunda of the known mass was placed under the left lateral lobe of the liver, and resection of this lobe was performed at 10 mm distance from the edge. The bleeding was stopped by application of the tested materials. The mass of blood loss (gravimetric parameters) and the time of bleeding were evaluated. The reliability of the differences was determined using nonparametric Mann-Whitney test.
RESULTS: In animals that were not administered the anticoagulant before modeling of the liver trauma, statistically significant differences were found only in such parameters as increase in the sample mass after impregnation with blood, in percent. Here, the value of this parameter in the group with use of hemostatic collagen sponge (2262.9) was three times that in the group using hemostatic Сhitocol-Hemo® (722.7) p = 0.000003. The differences between the groups with heparin therapy were of similar character (p = 000003).
CONCLUSION: The hemostatic effect of the sample of Сhitocol-Hemo® hemostatic agent was confirmed in an acute experiment on a model of liver injury in rats on the basis of measurement of the mass of blood lost, of blood absorbed by the sample, and also of bleeding time. This hemostatic effect is probably provided due to positive physicochemical characteristics (porous structure, stroma/pores ratio and composition of the agent.
全文:
作者简介
Vyacheslav Lipatov
Kursk State Medical University
Email: drli@yandex.ru
ORCID iD: 0000-0001-6121-7412
SPIN 代码: 1170-1189
Scopus 作者 ID: 57207347330
Researcher ID: D-8788-2013
MD, Dr. Sci. (Med.), Professor
俄罗斯联邦, KurskEduard Fronchek
Evers Group Rus
Email: fronchek6@yandex.ru
ORCID iD: 0000-0002-1778-3035
SPIN 代码: 7045-7306
Scopus 作者 ID: 6506995098
Cand. Sci. (Chemistry)
俄罗斯联邦, MoscowArsen Grigor'yan
Kursk State Medical University
Email: arsgrigorian@mail.ru
ORCID iD: 0000-0002-5039-5384
SPIN 代码: 3090-4890
Scopus 作者 ID: 56625648200
Researcher ID: E-5370-2013
MD, Cand. Sci. (Med.), Associate Professor
俄罗斯联邦, KurskDmitriy Severinov
Kursk State Medical University
编辑信件的主要联系方式.
Email: dmitriy.severinov.93@mail.ru
ORCID iD: 0000-0003-4460-1353
SPIN 代码: 1966-0239
Scopus 作者 ID: 57192996740
Researcher ID: G-4584-2017
MD, Cand. Sci. (Med.)
俄罗斯联邦, KurskM. David Z. Naimzada
Kursk State Medical University
Email: david.kursk@gmail.com
ORCID iD: 0000-0002-7894-6029
SPIN 代码: 8645-6468
Scopus 作者 ID: 57209744761
Researcher ID: A-1521-2016
俄罗斯联邦, Kursk
Lyudmila Zakutayeva
Kursk State Medical University
Email: mila.zakutayeva46@mail.ru
ORCID iD: 0000-0002-7204-1851
SPIN 代码: 1861-9637
Researcher ID: G-4524-2019
俄罗斯联邦, Kursk
参考
- Базаев А.В., Алейников А.В., Королев С.К., и др. Повреждение печени и селезенки у пострадавших с сочетанной автодорожной травмой // Журнал МедиАль. 2014. № 1 (11). С. 17–19.
- Abri B., Vahdati S.S., Paknezhad S., et al. Blunt abdominal trauma and organ damage and its prognosis // Journal of Analytical Research in Clinical Medicine. 2016. Vol. 4, № 4. P. 228–232. doi: 10.15171/jarcm.2016.038
- Cao S., Xu G., Li Q., et al. Double crosslinking chitosan sponge with antibacterial and hemostatic properties for accelerating wound repair // Composites. Part B: Engineering. 2022. Vol. 234, № 3. P. 109746. doi: 10.1016/j.compositesb.2022.109746
- Vecchio R., Catalano R., Basile F., et al. Topical hemostasis in laparoscopic surgery // Il Giornale di Chirurgia. 2016. Vol. 37, № 6. P. 266–270. doi: 10.11138/gchir/2016.37.6.266
- Самохвалов И.М., Рева В.А., Денисов А.В., и др. Сравнительная оценка эффективности и безопасности местных гемостатических средств в эксперименте // Военно-медицинский журнал. 2017. Т. 338, № 2. С. 18–24. doi: 10.17816/RMMJ73274
- Abbasipour M., Mirjalili M., Khajavi R., et al. Coated Cotton Gauze with Ag/ZnO/Chitosan Nanocomposite as a Modern Wound Dressing // Journal of Engineered Fibers and Fabrics. 2014. Vol. 9, № 1. P. 124–130. doi: 10.1177/155892501400900114
- Güven H.E. Topical hemostatics for bleeding control in pre-hospital setting: then and now // Ulusal Travma ve Acil Cerrahi Dergisi. 2017. Vol. 23, № 5. P. 357–361. doi: 10.5505/tjtes.2017.47279
- Sergi R., Bellucci D., Salvatori R., et al. Chitosan-Based Bioactive Glass Gauze: Microstructural Properties, In Vitro Bioactivity, and Biological Tests // Materials (Basel). 2020. Vol. 13, № 12. P. 2819. doi: 10.3390/ma13122819
- Давыденко В.В., Власов Т.Д., Доброскок И.Н., и др. Сравнительная эффективность аппликационных гемостатических средств местного действия при остановке экспериментального паренхиматозного и артериального кровотечения // Вестник экспериментальной и клинической хирургии. 2015. Т. 8, № 2. С. 186–194. doi: 10.18499/2070-478X-2015-8-2-186-194
- Wang L.–L., Wang S.–X. Research progress and application status of topical absorbable hemostatic // Journal of Medical Postgraduates. 2018. Vol. 31, № 1. P. 109–112. doi: 10.16571/j.cnki.1008-8199.2018.01.023
- Липатов В.А., Северинов Д.А., Крюков А.А., и др. Этические и правовые аспекты проведения экспериментальных биомедицинских исследований in vivo. Часть II // Российский медико-биологический вестник имени академика И.П. Павлова. 2019. Т. 27, № 2. С. 245–257. doi: 10.23888/PAVLOVJ2019272245-257
- Липатов В.А., Гаврилюк В.П., Северинов Д.А., и др. Оценка эффективности гемостатических материалов в остром эксперименте in vivo // Анналы хирургической гепатологии. 2021. Т. 26, № 2. С. 137–143. doi: 10.16931/1995-5464.2021-2-137-143
- Самохвалов И.М., Рева В.А., Пронченко А.А., и др. Местные гемостатические средства: новая эра в оказании догоспитальной помощи // Политравма. 2013. № 1. С. 80–86.
- Tang F., Lv L., Lu F., et al. Preparation and characterization of N-chitosan as a wound healing accelerator // International Journal of Biological Macromolecules. 2016. Vol. 93, Pt. A. P. 1295–1303. doi: 10.1016/j.ijbiomac.2016.09.101
- Ляпина Л.А., Григорьева М.Е., Ляпин Г.Ю., и др. Агрегационные эффекты хитозана в крови // Norwegian Journal of Development of the International Science. 2021. № 61. С. 13–16.