Modeling of fibrocystic mastopathy in experiment on animals

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Nowadays, hormonal imbalance is proven to be a factor that influences initiation of malignant and benign breast tumors. To study the aspects of participation of sex hormones in damage to organs and tissues, it may be necessary to model a common women’s pathology – fibrocystic disease of mammary glands characterized by the most pronounced effects of this pathogenetic factor, on experimental animals.

Aim. To create a model of fibrocystic disease of mammary gland with the subsequent possibility of studying morphological manifestations of the disease in natural and drug-induced pathomorphism.

Materials and Methods. The pathology was induced by intramuscular injection of 0.5 ml of 2% synestrol and 0.5 ml of 2.5% progesterone to virgin female rats on the 1st, 7th, 14th, 21st, 28th and 35th days of the experiment. For examination, histological preparations of inguinal mammary glands were made. The preparations were described and studied using morphometric analysis.

Results. In the result of the experiment, pronounced macro- and microscopic alterations of mammary glands were found. Microscopic picture was similar to that observed in fibrocystic mastopathy in women. Almost all the morphometric parameters underwent reliable alterations in correspondence with the given pathology.

Conclusion. A model of fibrocystic disease of mammary gland was obtained that may be used for further study of morphogenesis and methods of correction.

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作者简介

Svetlana Anisimova

Ryazan State Medical University

编辑信件的主要联系方式.
Email: anisimovasvetla@yandex.ru
ORCID iD: 0000-0002-5486-6753
SPIN 代码: 2501-4891
Researcher ID: AAF-8526-2020

MD, PhD, Associate Professor of the Department of Histology, Pathological Anatomy and Medical Genetics

俄罗斯联邦, Ryazan

Zhanna Svirina

Ryazan State Medical University

Email: anto-vasin@inbox.ru
ORCID iD: 0000-0001-5895-231X
SPIN 代码: 4617-1817
Researcher ID: D-2931-2018

MD, PhD, Assistant Lecturer of the Department of Pathophysiology

俄罗斯联邦, 9, Vysokovoltnaja, Ryazan, 390026

Denis Maksaev

Ryazan State Medical University

Email: denma1804@yandex.ru
ORCID iD: 0000-0003-3299-8832
SPIN 代码: 9962-2923

PhD-Student of the Department of Cardiovascular, X-ray Endovascular, Operative Surgery and Topographic Anatomy

俄罗斯联邦, Ryazan

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2. Fig. 1. Macroscopic photographs of the mammary glands of rats: (а) Control (intact) group and (b) experimental group

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3. Fig. 2. Histological images of the mammary glands of rats: (а) Normal and (b) with fibrocystic disease. Staining with hematoxylin and eosin, 40× magnification

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