Experimental study on disorders in the activity of macrophages in the acute period of different severities of cerebral stroke
- 作者: Kulchikov A.E.1, Morozov S.G.1, Musin R.S.2, Grinenko E.A.3
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隶属关系:
- Institute of General Pathology and Pathophysiology
- A.I. Evdokimov Moscow State University of Medicine and Dentistry
- N.N. Burdenko Neurosurgery Institute
- 期: 卷 29, 编号 2 (2021)
- 页面: 201-212
- 栏目: Original study
- ##submission.dateSubmitted##: 09.01.2021
- ##submission.dateAccepted##: 16.02.2021
- ##submission.datePublished##: 22.07.2021
- URL: https://journals.eco-vector.com/pavlovj/article/view/57821
- DOI: https://doi.org/10.17816/PAVLOVJ57821
- ID: 57821
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BACKGROUND: The development of immunosuppression in the acute phase of cerebral stroke may lead to infectious and inflammatory complications. However, data on the functional activity of macrophages are insufficient to understand the pathogenesis of stroke.
AIM: This study aimed to investigate the functional activity of macrophages in the acute period of experimental cerebral stroke (ECS) of different severities.
MATERIALS AND METHODS: In an experimental study on 45 Wistar rats, the functional and spontaneous metabolic activity of macrophages in the spleen and abdominal cavity and the induced metabolic activity of peritoneal macrophages were evaluated through the reduction of nitro blue tetrazolium via spectrophotometry. The spontaneous and induced adhesive activity and the inhibition of the modified adhesive activity of peritoneal macrophages were examined via crystal violet staining by using a colorimetric method. ECS was modeled by the rotational movements of mandrel-knife inserted into the region of capsula interna (c.i.) on the left by guiding a needle–cannula with the subsequent damage of vessels and the formation of mild (local incision of vessels in the region of c.i.), moderate (additional introduction of autologous blood in the area of c.i.), and severe (cutting of vessels from c.i. to pia mater with the subsequent introduction of autologous blood into the damaged area) hemorrhage. Data were evaluated in 72 h after different severities of ECS were modeled.
RESULTS: In 72 h after ECS with different severities was modeled, the functional and spontaneous metabolic activity of splenic macrophages significantly decreased compared with those in the control group. The functional, spontaneous, and induced metabolic activity of peritoneal macrophages significantly reduced compared with those in the control group. The adhesive activity of peritoneal macrophages significantly increased compared with that of the control group. These disorders exacerbated in animal models with a more severe degree of stroke.
CONCLUSION: In the acute period of ECS, the functional activity of macrophages decreased, whereas their adhesive activity increased. These phenomena enhanced in more severe models of stroke.
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作者简介
Andrey Kulchikov
Institute of General Pathology and Pathophysiology
编辑信件的主要联系方式.
Email: andrey.kulchikov@gmail.com
ORCID iD: 0000-0003-0470-1245
SPIN 代码: 7624-8471
Researcher ID: AAA-3072-2021
MD, Cand.Sci.(Med.), Senior Researcher of the General and Perinatal Neuroimmunopathology Laboratory
俄罗斯联邦, MoscowSergey Morozov
Institute of General Pathology and Pathophysiology
Email: andrey.kulchikov@gmail.com
ORCID iD: 0000-0001-5822-5729
Researcher ID: K-2503-2018
MD, Dr.Sci.(Med.), Professor, Head of the General and Perinatal Neuroimmunopathology Laboratory
俄罗斯联邦, MoscowRashit Musin
A.I. Evdokimov Moscow State University of Medicine and Dentistry
Email: rashel.63@mail.ru
ORCID iD: 0000-0001-9205-6658
SPIN 代码: 3469-9687
Researcher ID: AAA-7444-2021
MD, Dr. Sci.(Med.), Professor of the Neurology Department
俄罗斯联邦, MoscowElena Grinenko
N.N. Burdenko Neurosurgery Institute
Email: e_grinenko2000@mail.ru
ORCID iD: 0000-0002-3518-2217
SPIN 代码: 2035-5695
Researcher ID: AAA-3559-2021
MD, Dr.Sci.(Med.), Scientist of the Intensive Care Department
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