Immunohistochemical evaluation of apoptosis inducing factor in missed abortion after IVF

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Abstract

BACKGROUND: Apoptosis is involved both in the cyclic transformation and the functional activity of the endometrium during the menstrual cycle. Upon the onset of pregnancy, apoptosis is an important component of the placentation process. Moreover, changes in the apoptosis-inducing factor expression in the gravidar endometrium may lead to peculiarity both of trophoblast differentiation and the gestation course, regardless of the conception method.

AIM: to study the expression of apoptosis-inducing factor in abortive material in non-developing pregnancy after IVF.

MATERIALS AND METHODS: Histological and immunohistochemical analysis of the apoptosis-inducing factor expression were performed in 77 gravidar endometrium samples in patients with IVF pregnancy loss at 5–8 weeks and in 15 aborted materials that were received after abortion at similar week progression of pregnancy.

RESULTS: Significant decrease in the expression apoptosis-inducing factor in gravidar endometrium of the control group was revealed in comparison with the same parameter of the main group regardless of gravidar endometrium transformation variation. The highest expression apoptosis-inducing factor was detected in the main group gravidar endometrium that consists secretive and proliferative glands and incomplete gravidar transformation of the stroma.

CONCLUSIONS: High apoptosis-inducing factor expression both in the complete and incomplete gravidar transformation of the endometrium in aborted material after IVF pregnancy loss indicates the pathological apoptosis activation. Thus, the pathological apoptosis initiation occurs even before pregnancy onset in patients with infertility, endometrial dysfunction and chronic inflammatory process in the endometrium.

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About the authors

Tatiana G. Tral

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University

Author for correspondence.
Email: ttg.tral@yandex.ru

MD, PhD, Senior researcher, Pathomorphology Department

Russian Federation, Saint Petersburg; Saint Petersburg

Gulruchsor Kh. Tolibova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; North-Western State Medical University named after I.I. Mechnikov

Email: gulyatolibova@yandex.ru

MD, PhD, Dr. Med. Sci., Leading Scientist, Head of the Pathomorphology Department

Russian Federation, Saint Petersburg; Saint Petersburg

Igor Yu. Kogan

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: ikogan@mail.ru

MD, PhD, Dr. Med. Sci., Director

Russian Federation, Saint Petersburg

References

  1. Tral TG, Tolibova GH, Serdyukov SV, et al. Morphofunctional assessment of the causes of frozen pregnancy in the first trimester. Journal of Obstetrics and women’s Diseases. 2013;62(3):83-87. (In Russ.) doi: 10.17816/JOWD62383-87
  2. Mikhaleva LM, Boltovskaya MN, Mikhalev SA, et al. Endometrial dysfunction caused by chronic endometritis: сlinical and morphological aspects. Arkhiv patologii. 2017;79(6):22–29. (In Russ.) doi: 10.17116/patol201779622-29
  3. Peretiatko LP, Fateeva NV, Kuznetsov RA, Malyshkina AI. Vascularization of chorion villi in the first trimester of gestation with physiological course and in recurrent miscarriage with underlying chronic endometritis. I.P. Pavlov Russian Medical Biological Herald. 2017;25(4):612–620. (In Russ.) doi: 10.23888/PAVLOVJ20174612-620
  4. Radzinskii VE, editor. Nerazvivayushchayasya beremennost’. Moscow: GEHOTAR-Media, 2019. 176 p. (In Russ.)
  5. Tapil’skaya NI, Kogan IYu, Gzgzyan AM. Vedenie beremennosti rannikh srokov, nastupivshei v rezul’tate protokolov VRT. Rukovodstvo dlya vrachei. Moscow: GEHOTAR-Media, 2020. 144 p. (In Russ.)
  6. Tolibova GKh, Tral TG, Kleshchev MA. Ehndometrial’naya disfunktsiya: algoritm gistologicheskogo i immunogistokhimicheskogo issledovaniya. Uchebnoe posobie dlya vrachei. Saint Petersburg, 2016. (In Russ.)
  7. Tral TG, Tolibova GKh. Morphological variants of decidual endometrial transformation in missed abortion after in vitro fertilization. Clinical and Experimental Morphology. 2021;10(S4):42–51. (In Russ.) doi: 10.31088/CEM2021.10.S4.42-51
  8. Bakri NM, Ibrahim SF, Osman NA, et al. Embryo apoptosis identification: Oocyte grade or cleavage stage? Saudi J Biol Sci. 2016;23(1S):50–55. doi: 10.1016/j.sjbs.2015.10.023
  9. Betts DH, King WA. Genetic regulation of embryo death and senescence. Theriogenology. 2001;55(1): 171–191. doi: 10.1016/s0093-691x(00)00453-2
  10. Di Pietro C, Cicinelli E, Guglielmino MR, et al. Altered transcriptional regulation of cytokines, growth factors, and apoptotic proteins in the endometrium of infertile women with chronic endometritis. Am J Reprod Immunol. 2013;69(5):509–517. doi: 10.1111/aji.12076
  11. El Hajj N, Haaf T. Epigenetic disturbances in in vitro cultured gametes and embryos: implications for human assisted reproduction. FertilSteril. 2013;99(3): 632–641. doi: 10.1016/j.fertnstert.2012.12.044
  12. Makri D, Efstathiou P, Michailidou E, Maalouf WE. Apoptosis triggers the release of microRNA miR-294 in spent culture media of blastocysts. J Assist Reprod Genet. 2020; 37:1685–1694. doi: 10.1007/s10815-020-01796-5
  13. Meresman GF, Olivares C, Vighi S, et al. Apoptosis is increased and cell proliferation is decreased in out-of-phase endometria from infertile and recurrent abortion patients. Reprod Biol Endocrinol. 2010;8:126. doi: 10.1186/1477-7827-8-126
  14. Ramos-Ibeas P, Gimeno I, Cañón-Beltrán K, et al. Senescence and Apoptosis During in vitro Embryo Development in a Bovine Model. Front Cell Dev Biol. 2020;8:619902. doi: 10.3389/fcell.2020.619902
  15. Riddell MR, Winkler-Lowen B, Guilbert LJ. The contribution of apoptosis-inducing factor (AIF) to villous trophoblast differentiation. Placenta. 2012;33(2):88–93. doi: 10.1016/j.placenta.2011.11.008
  16. Smith SC, Baker PN, Symonds EM. Increased placental apoptosis in intrauterine growth restriction. Am J Obstet Gynecol. 1997;177(6):1395–1401. doi: 10.1016/s0002-9378(97)70081-4
  17. Tseng Y-F, Cheng H-R, Chen Y-P, et al. Grief reactions of couples to perinatal loss: A one-year prospective follow-up. J Clin Nurs. 2017;26(23–24):5133–5142. doi: 10.1111/jocn.14059
  18. Li Y-H, Wang H-L, Zhang Y-I. Expression of AIF-1 and RANTES in Unexplained Spontaneous Abortion and Possible Association with Alloimmune Abortion. Journal of Reproduction and Contraception. 2007;18(4): 261–270. doi: 10.1016/S1001-7844(07)60032-7

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Copyright (c) 2022 Tral T.G., Tolibova G.K., Kogan I.Y.

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