Possibilities of using allogeneic mesenchymal stem cells and wound dressings based on aliphatic copolyamide in microautodermoplasty
- Authors: Gordienko V.A.1, Zinoviev E.V.1,2, Kostyakov D.V.2, Asadulaev M.S.3, Shabunin A.S.3,4, Yudin V.E.4, Smirnova N.V.4,5, Radeeva A.V.1, Paneiakh M.B.1
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Affiliations:
- Saint Petersburg State Pediatric Medical University
- Saint Petersburg I.I. Dzhanelidze Research Institute of Emergency Medicine
- H. Turner National Medical Research Center for Сhildren’s Orthopedics and Trauma Surgery
- Peter the Great Saint Petersburg Polytechnic University
- Institute of Macromolecular Compounds of the Russian Academy of Sciences
- Issue: Vol 8, No 2 (2020)
- Pages: 185-196
- Section: Experimental and theoretical research
- URL: https://journals.eco-vector.com/turner/article/view/25751
- DOI: https://doi.org/10.17816/PTORS25751
- ID: 25751
Cite item
Abstract
Background. The treatment of victims with wound defects is an urgent problem of clinical medicine that doctors of various specialties, mainly surgeons and traumatologists, must face. Regardless of the etiology of the traumatic agent, the wound process is always subjected to fundamental pathophysiological processes. Despite the advances made by medical science in the local treatment of wounds (cell technology, modern wound coverings, and others), surgical procedures remain the main methods, and the search for new techniques to optimize reparative regeneration continues. This ongoing search indicates the absence of a universal algorithm for treating such defects. This lack of a universal treatment algorithm is of particular importance for assisting victims with extensive defects, which often leads to a shortage of donor resources.
Aim. The aim of this study was to increase the efficiency of microautodermoplasty due to the use of allogeneic mesenchymal stem cells and wound dressings based on aliphatic copolyamide.
Materials and methods. This paper presents the results of an experimental study involving 50 rats. All animals were divided into groups considering the choice of the method of the local treatment. The experimental wound was modeled according to its original technique. The evaluation of the effectiveness of the analyzed methods was performed using planimetric and histological research methods, and by calculating the healing index.
Results. The most effective methods for treating experimental wounds using microautodermoplasty (MADP) are wound dressings based on aliphatic copolyamide (CoPA) and adipogenic mesenchymal stem cells (AMSC). By 28 days of treatment after performing MADP + CoPA wound dressings + AMSC, it was possible to reduce the defect area by 16 times compared with the control, and the healing index was the maximum value among all methods — 12.5 units. The high regenerative potential was also confirmed by the results of the histological examination. The worst results were found in the MADP group with AMSK that did not cover the wounds with skin or wound dressing.
Conclusion. The introduction of the analyzed methods into clinical practice will improve the results of treatment of patients with wound defects of various etiologies.
Full Text
About the authors
Vasily A. Gordienko
Saint Petersburg State Pediatric Medical University
Email: chet1337@gmail.com
ORCID iD: 0000-0003-0590-2137
SPIN-code: 4069-2346
MD, assistant at the Laboratory of Experimental Surgery
Russian Federation, 2, Litovskay street, Saint-Peterburg, 194100Evgenii V. Zinoviev
Saint Petersburg State Pediatric Medical University; Saint Petersburg I.I. Dzhanelidze Research Institute of Emergency Medicine
Email: evz@list.ru
ORCID iD: 0000-0002-2493-5498
SPIN-code: 4069-2346
MD, PhD, D.Sc., Professor, Head of the Department of Thermal Injuries Unit; Head of the Laboratory of Experimental Surgery
Russian Federation, 2, Litovskay street, Saint-Peterburg, 194100; 3, Budapeshtskaya street, Saint-Petersburg, 192242Denis V. Kostyakov
Saint Petersburg I.I. Dzhanelidze Research Institute of Emergency Medicine
Author for correspondence.
Email: kosdv@list.ru
ORCID iD: 0000-0001-5687-7168
SPIN-code: 9966-5821
MD, PhD, researcher of the Department of Thermal Injuries Unit
Russian Federation, 3, Budapeshtskaya street, Saint-Petersburg, 192242Marat S. Asadulaev
H. Turner National Medical Research Center for Сhildren’s Orthopedics and Trauma Surgery
Email: marat.asadulaev@yandex.ru
ORCID iD: 0000-0002-1768-2402
SPIN-code: 3336-8996
Scopus Author ID: 57191618743
MD, clinical resident, laboratory assistant in the Laboratory of Experimental Surgery
Russian Federation, 64, Parkovaya str., Saint-Petersburg, Pushkin, 196603Anton S. Shabunin
H. Turner National Medical Research Center for Сhildren’s Orthopedics and Trauma Surgery; Peter the Great Saint Petersburg Polytechnic University
Email: anton-shab@yandex.ru
ORCID iD: 0000-0002-8883-0580
SPIN-code: 1260-5644
Scopus Author ID: 57191623923
laboratory assistant in the Laboratory of Experimental Surgery; PhD student
Russian Federation, 64, Parkovaya str., Saint-Petersburg, Pushkin, 196603; 29, Polytechnitcheskaya street, St.-Petersburg, 195251Vladimir E. Yudin
Peter the Great Saint Petersburg Polytechnic University
Email: yudin@hq.macro.ru
ORCID iD: 0000-0002-5517-4767
SPIN-code: 4996-7540
Scopus Author ID: 7103377720
Dr. Phys.-Math. Sci., Professor, Director of Laboratory of Polymeric Materials for Tissue Engeneering and Transplantology
Russian Federation, 29, Polytechnitcheskaya street, St.-Petersburg, 195251Nataliya V. Smirnova
Peter the Great Saint Petersburg Polytechnic University; Institute of Macromolecular Compounds of the Russian Academy of Sciences
Email: nvsmirnoff@yandex.ru
PhD, Senior Researcher of Laboratory of Polymer Materials for Tissue Engineering and Transplantology; researcher in Laboratory of Mechanics of Polymers and Composite Materials
Russian Federation, 29, Polytechnitcheskaya street, St.-Petersburg, 195251; 31, Bolshoy prospect, St-Petersburg 199004Anna V. Radeeva
Saint Petersburg State Pediatric Medical University
Email: anyawinteranya@gmail.com
ORCID iD: 0000-0002-6152-4276
student
Russian Federation, 2, Litovskay street, Saint-Peterburg, 194100Moisei B. Paneiakh
Saint Petersburg State Pediatric Medical University
Email: moisey031190@gmail.com
ORCID iD: 0000-0002-2527-9058
Assistant Professor, Department of Pathological Anatomy at the Rate of Forensic Medicine
Russian Federation, 2, Litovskay street, Saint-Peterburg, 194100References
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