Цифровая флуоресцентная микроскопия: новый аналитический инструмент для изучения микроорганизмов
- Авторы: Пучков Е.О1,2
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Учреждения:
- Институт биохимии и физиологии микроорганизмов имени Г.К.Скрябина РАН Федерального исследовательского центра «Пущинский научный центр биологических исследований РАН»
- Пущинский государственный естественно-научный институт
- Выпуск: № 8 (2021)
- Страницы: 3-15
- Раздел: Статьи
- URL: https://journals.eco-vector.com/0032-874X/article/view/628015
- DOI: https://doi.org/10.7868/S0032874X21080019
- ID: 628015
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Аннотация
За последние двадцать лет флуоресцентная микроскопия трансформировалась из субъективного визуального метода в объективный аналитический подход — цифровую флуоресцентную микроскопию (ЦФМ). Произошло это благодаря совокупности инноваций, связанных с модернизацией микроскопов, использованием компьютерной обработки и анализа цифровых изображений, а также применением новых флуорофоров и методов их внедрения в клетки микроорганизмов. ЦФМ открыла принципиально новые возможности для изучения микроорганизмов, которые позволяют получать уникальную информацию о локализации и динамике внутриклеточных процессов при изучении единичных клеток микроорганизмов на субклеточном уровне.
Об авторах
Е. О Пучков
Институт биохимии и физиологии микроорганизмов имени Г.К.Скрябина РАН Федерального исследовательского центра «Пущинский научный центр биологических исследований РАН»; Пущинский государственный естественно-научный институт
Email: puchkov@ibpm.pushchino.ru
Пущино, Россия; Пущино, Россия
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