AGE-RELATED ASPECTS OF THE SIRTUIN EXPRESSION LEVEL IN THE CARDIOMYOCYTES OF PATIENTS WITH DILATED CARDIOMYOPATHY
- Authors: Kravchenko K.P1, Kozlov K.L1,2, Polyakova V.O3,4, Medvedev D.S1,5
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Affiliations:
- Saint Petersburg Institute of Bioregulation and Gerontology
- S.M. Kirov Military Medical Academy
- Saint Petersburg Research Institute of Phthisiopulmonology, Ministry of Health of Russia
- Belgorod State National Research University
- Research Institute of Hygiene, Occupational Diseases, and Human Ecology, Federal Biomedical Agency of Russia
- Issue: Vol 33, No 10 (2022)
- Pages: 70-74
- Section: Articles
- URL: https://journals.eco-vector.com/0236-3054/article/view/114710
- DOI: https://doi.org/10.29296/25877305-2022-10-14
- ID: 114710
Cite item
Abstract
Sirtuins are among the signaling molecules that may have important prognostic value in dilated cardiomyopathy (DCM). Objective. To study the expression level of sirtuins in the cardiomyocytes of patients with DCM in vitro. Subjects and methods. The study used cardiomyocyte cultures taken during heart biopsy from 3 middle-aged male patients (mean age 52.3±2.6 years) with DCM. A culture of normal human cardiomyocytes served as a control. The investigators applied a primary dissociated cell culturing method and immunofluorescence confocal laser scanning microscopy. To simulate cellular senescence, they employed Passages 3 and 10 cells that corresponded to young and old cultures. Results. At the molecular level, cardiomyocyte aging was accompanied by a decrease in the expression of sirtuins 1, 3, and 6; whereas the expression of sirtuin 2 increased significantly in the old cultures versus the young ones in both the control and DCM groups. The findings suggest may suggest that sirtuins 1,2, 3, and 6 are involved not only in the pathogenesis of DCM, but also in the mechanisms of aging.
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About the authors
K. P Kravchenko
Saint Petersburg Institute of Bioregulation and Gerontology
Email: longtermcare.fmba@gmail.com
Russian Federation,
K. L Kozlov
Saint Petersburg Institute of Bioregulation and Gerontology; S.M. Kirov Military Medical Academy
Email: longtermcare.fmba@gmail.com
Professor, MD
Russian Federation,V. O Polyakova
Saint Petersburg Research Institute of Phthisiopulmonology, Ministry of Health of Russia; Belgorod State National Research University
Email: longtermcare.fmba@gmail.com
Professor, MD
Russian Federation,D. S Medvedev
Saint Petersburg Institute of Bioregulation and Gerontology; Research Institute of Hygiene, Occupational Diseases, and Human Ecology, Federal Biomedical Agency of Russia
Author for correspondence.
Email: longtermcare.fmba@gmail.com
Professor, MD
Russian Federation,References
- Anderson R., Lagnado A., Maggiorani D. et al. Length-independent telomere damage drives post-mitotic cardiomyocyte senescence. EMBO J. 2019; 38 (5): e100492. doi: 10.15252/embj.2018100492
- Anderson R., Richardson G.D., Passos J.F. Mechanisms driving the ageing heart. Exp Gerontol. 2018; 109: 5-15. doi: 10.1016/j.exger.2017.10.015
- Bykov A.T., Dyuzhikov A.A., Malyarenko T.N. Current views on age-related and dependent cardiovascular diseases. Medical Journal. 2015; 3: 7-12.
- Cardus A., Uryga A.K., Walters G. et al. SIRT6 protects human endothelial cells from DNA damage, telomere dysfunction, and senescence. Cardiovasc Res. 2013; 97: 571-9. doi: 10.1093/cvr/cvs352
- Elliott P., Andersson B., Arbustini E. et al. Classification of the cardiomyopathies: a position statement from the European Society of Cardiology Working Group on Myocardial and Pericardial Diseases. Eur Heart J. 2008; 29: 270-6. doi: 10.1093/eurheartj/ehm342
- Frescas D., Valenti L., Accili D. Nuclear trapping of the forkhead transcription factor FoxO1 via Sirt-dependent deacetylation promotes expression of glucogenetic genes. J Biol Chem. 2005; 280 (21): 20589-95. doi: 10.1074/jbc.M412357200
- Gerhart-Hines Z., Rodgers J.T., Bare O. Metabolic control of muscle mitochondrial function and fatty acid oxidation through SIRT1/PGC-1alpha. EMBO J. 2007; 26 (7): 1913-23. doi: 10.1038/sj.emboj.7601633
- Guzzo-Merello G., Cobo-Marcos M., Gallego-Delgado M. et al. Alcoholic cardiomyopathy. World J Cardiol. 2014; 6 (8): 771-81. doi: 10.4330/wjc.v6.i8.771
- Houtkooper R.H., Pirinen E., Auwerx J. Sirtuins as regu lators of metabolism and healthspan. Nat Rev Mol Cell Biol. 2012; 13 (4): 225-38. doi: 10.1038/nrm3293
- Japp A.G., Gulati A., Cook S.A. et al. The Diagnosis and Evaluation of Dilated Cardiomyopathy. J Am Coll Cardiol. 2016; 67 (25): 2996-3010. doi: 10.1016/j.jacc.2016.03.590
- Kanfi Y., Naiman S., Amir G. et al. The sirtuin SIRT6 regulates lifespan in male mice. Nature. 2012; 483 (7388): 218-21. doi: 10.1038/nature10815
- Kitamura Y.I., Kitamura T., Kruse J.P. FoxO1 protects against pancreatic beta cell failure through NeuroD and MafA induction. Cell Metab. 2005; 2 (3): 153-63. DOI: 10.1016/j. cmet.2005.08.004
- Lagouge M., Argmann C., Gerhart-Hines Z. Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1alpha. Cell. 2006; 127 (6): 1109-22. doi: 10.1016/j.cell.2006.11.013
- Liu J., Wu X., Wang X. et al. Global Gene Expression Profiling Reveals Functional Importance of Sirt2 in Endothelial Cells under Oxidative Stress.Int J Mol Sci. 2013; 14: 5633-49. doi: 10.3390/ijms14035633
- Lopez-Otin C., Blasco M.A., Partridge L. et al. The hallmarks of aging. Cell. 2013; 153 (6): 1194-217. doi: 10.1016/j.cell.2013.05.039
- McNally E.M., Mestroni L. Dilated Cardiomyopathy: Genetic Determinants and Mechanisms. Circ Res. 2017; 121 (7): 731-48. doi: 10.1161/CIRCRESAHA.116.309396
- Merlo M., Cannata A., Gobbo M. et al. Evolving concepts in dilated cardiomyopathy. Eur J Heart Fail. 2018; 20 (2): 228-39. doi: 10.1002/ejhf.1103
- Michan S., Sinclair D. Sirtuins in mammals: insights into their biological function. Biochem J. 2007; 404 (1): 1-13. doi: 10.1042/BJ20070140
- Moynihan K.A., Grimm A.A., Plueger M.M. Increased dosage of mammalian Sir2 in pancreatic beta cells enhances glucose-stimulated insulin secretion in mice. Cell Metab. 2005; 2 (2): 105-17. doi: 10.1016/j.cmet.2005.07.001
- Narayan N., Lee I.H., Borenstein R. et al. The NAD-dependent deacetylase SIRT2 is required for programmed necrosis. Nature. 2012; 492: 199-204. DOI: 10.1038/ nature11700
- North B.J., Rosenberg M.A., Jeganathan K.B. et al. SIRT2 induces the checkpoint kinase BubR1 to increase lifes-pan. EMBO J. 2014; 33: 1438-53. DOI: 10.15252/ embj.201386907
- O'Callaghan C., Vassilopoulos A. Sirtuins at the crossroads of stemness, aging, and cancer. Aging Cell. 2017; 16 (6): 1208-18. doi: 10.1111/acel.12685
- Parodi-Rullan R.M., Chapa-Dubocq X.R., Javadov S. Acetylation of Mitochondrial Proteins in the Heart: The Role of SIRT3. Front Physiol. 2018; 9: 1094. DOI: 10.3389/ fphys.2018.01094
- Picard F., Kurtev M., Chung N. Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-gamma. Nature. 2004; 429 (6993): 771-6. doi: 10.1038/nature02583
- Pinto Y.M., Elliott P.M., Arbustini E. et al. Proposal for a revised definition of dilated cardiomyopathy, hypokinetic non-dilated cardiomyopathy, and its implications for clinical practice: A position statement of the ESC working group on myocardial and pericardial diseases. Eur Heart J. 2016; 37: 1850-8. doi: 10.1093/eurheartj/ehv727
- Prozorovski T., Schulze-Topphoff U., Glumm R. Sirt1 contributes critically to the redox-dependent fate of neural progenitors. Nat Cell Biol. 2008; 10 (4): 385-94. DOI: 10.1038/ ncb1700
- Rodgers J.T., Lerin C., Haas W. et al. Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Nature. 2005; 434 (7029): 113-8. DOI: 10.1038/ nature03354
- Schultheiss H.P., Fairweather D., Caforio A.L.P. et al. Dilated cardiomyopathy. Nat Rev Dis Primers. 2019; 9 (5): 32. doi: 10.1038/s41572-019-0084-1
- Shimizu I., Minamino T. Cellular senescence in cardiac diseases. J Cardiol. 2019; 74 (4): 313-9. doi: 10.1016/j.jjcc.2019.05.002
- Sun C., Zhang F., Ge X. SIRT1 improves insulin sensitivity under insulin-resistant conditions by repressing PTP1B. Cell Metab. 2007; 6 (4): 307-19. DOI: 10.1016/j. cmet.2007.08.014
- Weintraub R.G., Semsarian C., Macdonald P. Dilated cardiomyopathy. Lancet. 2017; 390 (10092): 400-14. doi: 10.1016/S0140-6736(16)31713-5
- Xu Z., Zhang L., Fei X. et al. The miR-29b-Sirt1 axis regulates self-renewal of mouse embryonic stem cells in response to reactive oxygen species. Cell Signal. 2014; 26: 1500-5. doi: 10.1016/j.cellsig.2014.03.010
- Zhang, J., He Z., Fedorova J. Alterations in mitochondrial dynamics with age-related Sirtuin1/Sirtuin3 deficiency impair cardiomyocyte contractility. Aging Cell. 2021; 20 (7): e13419. doi: 10.1111/acel.13419
- Дедов Д.В. Комплексная профилактика возраст-ассоциированных и сердечнососудистых заболеваний: применение российского натурального препарата БиоДигидрокверцетин торговой марки «Байкальская Легенда». Врач. 2022; 33 (6): 64-7
- Обрезан А.Г., Куликов Н.В. Желудочковые экстрасистолии как причина кардио-миопатий. Медицинский альянс. 2018; 4: 70-5