AGE-RELATED ASPECTS OF THE SIRTUIN EXPRESSION LEVEL IN THE CARDIOMYOCYTES OF PATIENTS WITH DILATED CARDIOMYOPATHY
- 作者: Kravchenko K.P1, Kozlov K.L1,2, Polyakova V.O3,4, Medvedev D.S1,5
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隶属关系:
- Saint Petersburg Institute of Bioregulation and Gerontology
- S.M. Kirov Military Medical Academy
- Saint Petersburg Research Institute of Phthisiopulmonology, Ministry of Health of Russia
- Belgorod State National Research University
- Research Institute of Hygiene, Occupational Diseases, and Human Ecology, Federal Biomedical Agency of Russia
- 期: 卷 33, 编号 10 (2022)
- 页面: 70-74
- 栏目: Articles
- URL: https://journals.eco-vector.com/0236-3054/article/view/114710
- DOI: https://doi.org/10.29296/25877305-2022-10-14
- ID: 114710
如何引用文章
详细
Sirtuins are among the signaling molecules that may have important prognostic value in dilated cardiomyopathy (DCM). Objective. To study the expression level of sirtuins in the cardiomyocytes of patients with DCM in vitro. Subjects and methods. The study used cardiomyocyte cultures taken during heart biopsy from 3 middle-aged male patients (mean age 52.3±2.6 years) with DCM. A culture of normal human cardiomyocytes served as a control. The investigators applied a primary dissociated cell culturing method and immunofluorescence confocal laser scanning microscopy. To simulate cellular senescence, they employed Passages 3 and 10 cells that corresponded to young and old cultures. Results. At the molecular level, cardiomyocyte aging was accompanied by a decrease in the expression of sirtuins 1, 3, and 6; whereas the expression of sirtuin 2 increased significantly in the old cultures versus the young ones in both the control and DCM groups. The findings suggest may suggest that sirtuins 1,2, 3, and 6 are involved not only in the pathogenesis of DCM, but also in the mechanisms of aging.
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作者简介
K. Kravchenko
Saint Petersburg Institute of Bioregulation and Gerontology
Email: longtermcare.fmba@gmail.com
俄罗斯联邦,
K. Kozlov
Saint Petersburg Institute of Bioregulation and Gerontology; S.M. Kirov Military Medical Academy
Email: longtermcare.fmba@gmail.com
Professor, MD
俄罗斯联邦,V. Polyakova
Saint Petersburg Research Institute of Phthisiopulmonology, Ministry of Health of Russia; Belgorod State National Research University
Email: longtermcare.fmba@gmail.com
Professor, MD
俄罗斯联邦,D. Medvedev
Saint Petersburg Institute of Bioregulation and Gerontology; Research Institute of Hygiene, Occupational Diseases, and Human Ecology, Federal Biomedical Agency of Russia
编辑信件的主要联系方式.
Email: longtermcare.fmba@gmail.com
Professor, MD
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