Lipid markers of cervical epithelium neoplastic transformation in HPV-associated diseases


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Abstract

Objective. To discover peculiarities of the lipid composition of neoplastically transformed and adjacent cervical epithelium with HPV-associated diseases. Material and methods. The study included 41 patients with neoplastic transformations of the cervical epithelium, caused by human papillomavirus, who applied to outpatient department of Federal State Budget Institution “National Medical Research Center for Obstetrics, Gynecology and Perinatology” Ministry Of Healthcare of the Russian Federation. А biopsy of the cervix uteri (strictly according to the indications for biopsy) with subsequent histological examination of the biopsy specimens and analysis of their lipid composition were performed in all women, included in the study. Qualitative and quantitative assessment of biopsy samples lipid extracts was performed by mass spectrometry with electrospray ionization. Lipids of five classes were identified: phosphatidylcholines, phosphatidylethanolamines, sphingomyelins, di - and triglycerides. The obtained experimental data were analyzed using the multivariate OPLS-DA method. Results. A statistically meaningful change in the levels of lipids, such as phosphatidylcholines (PC 32:0, PC 34:1, PC 36:4, PC 34:0, PC 38:4), phosphatidylethanolamines (PE 0-46:0, LPE 46:0, PE 0-48:0, LPE 48:0, PE O-46:1) and sphingomyelins (SM 34:0, SM 42:2)was identified in cervical tissues with neoplastic transformation, caused by human papillomavirus. Conclusion. This study revealed significant changes in the content of several glycerophospholipids and sphingo-lipids in neoplastically transformed tissues of the cervix uteri compared to adjacent normal tissues. Found lipids can be used as a part of the prognostic panel to predict the course of cervical neoplasias in the direction of regress or malignancy.

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About the authors

Maria Evgenievna Nekrasova

National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia named after Academician V.l. Kulakov

Email: mashenka_90@mail.ru
PhD student

Vitaliy Viktorovich Chagovets

National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia named after Academician V.l. Kulakov

Email: vvchagovets@gmail.com
PhD, Senior Researcher of Laboratory of Proteomics and Metabolomics of Human Reproduction

Nataliia Leonidovna Starodubtseva

National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia named after Academician V.l. Kulakov

Email: n_starodubtseva@oparina4.ru
PhD, Head of Laboratory of Proteomics and Metabolomics of Human Reproduction

Alexey Sergeevich Kononikhin

National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia named after Academician V.l. Kulakov

Email: konoleha@yandex.ru
PhD, Researcher of Laboratory of Proteomics and Metabolomics of Human Reproduction

Dinara Failevna Salimova

National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia named after Academician V.l. Kulakov

Email: Salimova.l993@mail.ru
specialist of Laboratory of Proteomics and Metabolomics of Human Reproduction

Alisa Olegovna Tokareva

Moscow Institute of physics and technology

Email: alisa.tokareva@phystech.edu
postgraduate student

Vadim Vladimirovich Lagutin

National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia named after Academician V.l. Kulakov

Email: laggi@mail.ru
Researcher of Bioinformatics Laboratory

Vladimir Aleksandrovich Naumov

National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia named after Academician V.l. Kulakov

Email: looongdog@gmail.com
researcher of Bioinformatics Laboratory

Niso Mirzoevna Nazarova

National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia named after Academician V.l. Kulakov

Email: grab2@yandex.ru
MD, PhD, Senior Researcher

Vladimir Evgenievich Frankevich

National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia named after Academician V.l. Kulakov

Email: v_frankevich@oparina4.ru
PhD, Head of Department of Systems Biology in Reproduction

Gennadiy Tikhonovich Sukhikh

National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia named after Academician V.l. Kulakov

Email: g_sukhikh@oparina4.ru
Academician of RAMS, MD, PhD, Professor, Director

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