The expression profile of placental microRNAs as regulators of oxidative stress in fetal growth restriction


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Abstract

Objective. To evaluate the profile of differentially expressed microRNAs associated with oxidative stress and placental vascular dysfunction in fetal growth restriction (FGR). Materials and methods. Real-time quantitative RT-PCR was used to evaluate the placental tissue expression of microRNAs: miR-16-5p, miR-26b-5p, miR-100-5p, miR-125b-5p, miR-146a-5p, miR-182-5p, miR-199a-5p, miR-221-3p, miR-451a, and miR-574-3p in pregnant women with early- and late-onset FGR and control groups in the corresponding period. Results. Placental tissues from pregnant women with FGR were found to have signif icant changes in the expression levels of the microRNAs playing an important role in the regulation of oxidative stress: miR-125b-5p, miR-221-3p, miR-451a, and miR-574-3P in early-onset FGR and miR-451a and miR-574-3p in late-onset FGR. The DAVID database.v.6.8 could identify the potential targets of the above microRNAs that are determined as the key participants of a number of biological pathways: TGFβ-, TNFα-, VEGF-, HIF1α-, FOXO-, and MAPK-signaling pathways. Conclusion. The evaluation of the expression of the tissue-specific microRNAs regulating the balanced work of pro- and antioxidant system genes allows further determination of their diagnostic potential in the umbilical cord plasma and blood from pregnant women with FGR.

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About the authors

Vladyslava A. Gusar

National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russian Federation

Email: v_gusar@oparina4.ru
PhD, senior researcher of Laboratory of Applied Transriptomics, Department of System Biology in Reproduction

Angelika V. Timofeeva

National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russian Federation

Email: v_timofeeva@oparina4.ru
PhD, senior researcher of Laboratory of Applied Transriptomics, Department of System Biology in Reproduction

Natalia E. Kan

National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russian Federation

Email: n_kan@oparina4.ru
MD, professor, head of Department of Obstetric Observatory

Vitaliy V. Chagovets

National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russian Federation

Email: v_chagovets@oparina4.ru
PhD, senior researcher of Laboratory of Proteomics and Metabolomics of Human Reproduction, Department of Systems Biology in Reproduction

Maria B. Ganichkina

National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russian Federation

Email: m_ganichkina@oparina4.ru
graduate student

Vladimir E. Frankevich

National Medical Research Center for Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russian Federation

Email: v_frankevich@oparina4.ru
PhD, head of Department of Systems Biology in Reproduction

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