Technological platforms for preimplantation genetic testing for aneuplody: comparative effectiveness of diagnosing chromosomal abnormalities


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Abstract

Objective. To analyze a range of chromosomal abnormalities in 5-6-day-old embryos using array comparative genomic hybridization (aCGH) and next-generation sequencing (NGS) methods in two centers for assisted reproductive technologies (ART) and to compare the results after applying two methods. Materials and methods. Trophectoderm samples of 1041 embryos were studied. NSG was performed on the platform Illumina MiSeq with VeriSeq PGS Library Prep Kit (Illumina); aCGH was conducted using microarray kit G5963A, Agilent. Results. Single chromosome aneuploidy rate starts increasing after 30 years of age, and aneuploidy involving two or more chromosomes after 37 years. In our sampling aCGH and NGS methods showed a similar effectiveness for performing PGT-A. Statistically significant differences in the incidence of chromosomal abnormalities between two ART centers were not revealed. Conclusion. The decrease in the proportion of embryos with balanced chromosome complement is the most important factor which reduces reproductive potential of women of advanced reproductive age undergoing IVF. Both methods aCGH and NGS demonstrate a similar effectiveness for performing PGT-A. The incidence of chromosomal abnormalities of various types did not differ in embryos cultivated in two IVF centers which took part in the investigation.

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About the authors

O. V Malysheva

FSBSI «The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott»;FSBSI Pavlov Institute of Phisioligy

Email: omal99@mail.ru
senior researcher, laboratory of genomics, senior researcher, laboratory of neuroendocrinology 199034, Mendeleyevskaya liniya, 3, St.Petersburg, Russia, tel. +7(812) 3289809; 199034, 3, Makarova enb, St.Petersburg, Russia, tel. +7(812) 7(812) 328-07-01

N. K Bichevaya

Russia international Center for Reproductive Medicine

Email: bichevaya@mcrm.ru
Head of the Laboratory of Assisted Reproductive Technologies 53/1 Komendantskij prospect, St. Petersburg, 197350, Russian Federation

A. M Gzgzyan

FSBSI «The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott»

Email: agzgzyan@gmail.com
Head of ART Department 3 Mendeleyevskaya Line, St. Petersburg, 199034, Russian Federation

O. S Glotov

City Hospital №40; FSBSI «The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott»

Email: olglotov@mail.ru
Head of the Genetic Laboratory, senior researcher, laboratory of genomics St.-Petersburg, 9 Borisova Street, Sestrorezk, St. Petersburg, 197706, Russia; 199034, Mendeleyevskaya liniya, 3, St.Petersburg, Russia, tel. +7(812) 3289809

A. A Kinunen

St. Petersburg Centre for Medical Genetics; International centre of reproductive medicine

Email: kinunen@mcrm.ru
geneticist physician Tobolskaya St, 5, St. Petersburg, 194044, Russia; Komendantskij prospect, 53k1, St. Petersburg, 197350, Russia; tel. (812) 327-19-50

AAu. Yu Lobenskaya

Cerbalab LTD

Email: alobenskaya@cerbalab.ru
Head of the Laboratory, Genetic Laboratory 90-2 letter «Z», Bolshoy Ave. V. O., St. Petersburg, 199106, Russian Federation

I. D Mekina

FSBSI «The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott»

Email: irendf@mail.ru
Senior Researcher of ART Department 3 Mendeleyevskaya Line, St. Petersburg, 199034, Russian Federation

I. V Polyakova

City Hospital №40

Email: irena.88@inbox.ru
Biologist of the Genetic Laboratory 9 Borisova Street, Sestrorezk, St. Petersburg, 197706, Russian Federation

I. L Puppo

Almazov National Medical Research Centre of the Ministry of Health of the Russian Federation; International Centre for Reproductive Medicine

Associate Professor, Department of Laboratory Medicine and Genetics, Biologist, Laboratory of Assisted Reproductive Technologies 2 Akkuratova Street, St. Petersburg, 197341, Russia; 53/1 Komendantskij prospect, St. Petersburg, 197350, Russia; tel. +7(812)3271950

A4. F Saifitdinova

Herzen State Pedagogical University of Russia; Russia international Center for Reproductive Medicine

Email: saifitdinova@mail.ru
Associate Professor, Department of Human and Animal Anatomy and Physiology, Deputy Head of the Laboratory of Assisted Reproductive Technologies 48 Moyka Embankment, St. Petersburg, 191186, Russia; 53/1 Komendantskij prospect, St. Petersburg, 197350, Russia; tel. +7(812)3271950

S. G Shcherbak

City Hospital №40

Email: sgsherbak@mail.ru
Chief Physician 9 Borisova Street, Sestrorezk, St. Petersburg, 197706, Russian Federation.

I. Y.U Fogan

FSBSI «The Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott»

Email: ikogan@mail.ru
Director 3 Mendeleyevskaya Line, St. Petersburg, 199034, Russian Federation

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