Immunoregulatory and transport proteins as markers of antenatal prognosis of preterm infants’ status in women with preterm prelabor rupture of membranes
- Authors: Grigor’eva E.Y.1, Renge L.V.1, Zorina V.N.2, Bazhenova L.G.1, Likhacheva V.V.1
-
Affiliations:
- Novokuznetsk State Institute for Advanced Medical Studies, branch of Russian Medical Academy of Continuous Professional Education of Minzdrav of Russia
- State Research Institute for High-Purity Biological Preparations of the Federal Medical and Biological Agency of Russia
- Issue: No 3 (2020)
- Pages: 148-154
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/248913
- DOI: https://doi.org/10.18565/aig.2020.3.148-154
- ID: 248913
Cite item
Open Access
Access granted
Abstract
Aim. To investigate serum levels of lactoferrin (LF), alpha-2-macroglobulin (a2-MG), alpha-1-antitrypsin (a1-AT), and albumin and their association with neonatal outcomes in pregnant women with preterm prelabor rupture of membranes (PROM). Materials and methods. This study tested serum samples from women with PROM at 24-33 weeks’ gestation, who gave birth to preterm infants without signs of intrauterine infection (IUI) (n = 35), mild IUI (n = 30) and severe IUI (n = 28). A control group comprised 27 healthy women who were tested at 24-33 weeks’ gestation. Serum samples were tested for LFconcentration using ELISA; a2-MG, a1-ATwere determined by a quantitative rocket immunoelectrophoresis assay and albumin by the biochemical method. Results. Women with PROM, regardless of newborns’ health status, had statistically signif icantly higher serum levels of LF and lower levels of albumin than women in the control group. Serum levels of a2-MG and a1-AT in pregnant women with PROM, who gave birth to babies with no signs of IUI, did not differ from those of healthy pregnant women. But they were significantly higher in mothers of neonates with mild forms of IUI and, on the contrary, signif icantly lower in mothers of neonates with severe IUI. The discriminant analysis showed that in a pregnant woman with PROM, the most sensitive prognostic marker for IUI in the fetus and newborn was the serum level of a2-MG. Serum levels of a2-MG ranging from 2.2 to 3.0 g/l were 86% predictive of the birth of a child without IUI; a2-MG level>3.0 was 85% predictive of the birth of a child with a moderate IUI; a2-MG level <2.2 g/l was 86% predictive severe generalized forms of IUI in a newborn. Conclusion. Testing for serum levels of LF, a2-MG, a1-AT, and albumin in pregnant women with PROM can be used to predict their babies’ health status in the early neonatal period.
Full Text
About the authors
Ekaterina Yu. Grigor’eva
Novokuznetsk State Institute for Advanced Medical Studies, branch of Russian Medical Academy of Continuous Professional Education of Minzdrav of Russia
Email: prutovykh@icloud.com
Lyudmila V. Renge
Novokuznetsk State Institute for Advanced Medical Studies, branch of Russian Medical Academy of Continuous Professional Education of Minzdrav of Russia
Email: l.renge@mail.ru
Veronika N. Zorina
State Research Institute for High-Purity Biological Preparations of the Federal Medical and Biological Agency of Russia
Email: v.n.zorina@hpb.spb.ru
Lyudmila G. Bazhenova
Novokuznetsk State Institute for Advanced Medical Studies, branch of Russian Medical Academy of Continuous Professional Education of Minzdrav of Russia
Email: l_bagenova@mail.ru
VictoriaV. Vasil'evna Likhacheva
Novokuznetsk State Institute for Advanced Medical Studies, branch of Russian Medical Academy of Continuous Professional Education of Minzdrav of Russia
Email: viroli@mail.ru
References
- Айламазян Э.К., Кулаков В.И., Радзинский В.Е., Савельева Г.М. Акушерство: Национальное руководство. М: ГЭОТАР-Медиа 2009; 1200
- Артюхов И.П., Цхай В.Б., Капитонов В.Ф., Коновалов В.Н., Роговенко Е.Ю. Семейные медицинские проблемы, связанные с рождением и выхаживанием детей, родившихся с экстремально низкой массой тела. Сибирское медицинское обозрение. 2011; 3: 98-103
- Письмо Минздрава России от 17 декабря 2013 г. №15-4102-9480.
- Новикова В.А., Пенжоян Г.А., Рыбалка Е.В., Аутлева С.Р., Сикальчук О.И., Асеева Е.В. Роль инфекции в преждевременном разрыве плодных оболочек. Российский вестник акушера-гинеколога. 2012; 6: 35-9.
- Сидельникова В.М., Сухих Г.Т.Невынашивание беременности. М.: МИА 2010; 536.
- Ренге Л.В., Баженова Л.Г., Зорина В.Н., Зорина Р.М., Чирикова Т.С. Иммунорегуляторные белки в околоплодных водах при моно- и микстносительстве возбудителей перинатально значимых инфекций. Российский вестник акушера-гинеколога. 2015; 15(6): 17-23. doi: 10.17116/ rosakush201515617-23
- Лашкевич Е.Л., Захаренкова Т.Н., Барановская Е.И. Метод прогнозирования внутриутробной инфекции у новорожденных от женщин с недоношенной беременностью и преждевременным разрывом плод ных оболочек. Министерство Здравоохранения Республики Беларусь. Гомель 2014. Инструкция по применению № 142-1114 от 20 января 2015.
- КанН.Е., Сироткина Е.А., Тютюнник В.Л., Донников А.Е., Быстрицкий А.А., Кадочникова В.В., Маркелова Е.Г., Курчакова Т.А., Вересова А.А. Диагностическая роль клинических и молекулярно-генетических предикторов внутриутробной инфекции. Акушерство и гинекология. 2015; 4: 44-9.
- Oskovi Kaplan Z.A., Ozgu-Erdinc A.S. Prediction of Preterm Birth: Maternal Characteristics, Ultrasound Markers, and Biomarkers: An Updated Overview. J. Pregnancy. 2018: 8367571. doi: 10.1155/2018/8367571.
- Tchirikov M., Schlabritz-Loutsevitch N., Maher J., Buchmann J., Naberezhnev Y., Winarno A.S., Seliger G. Mid-trimester preterm premature rupture of membranes (PPROM): etiology, diagnosis, classification,international recommendations of treatment options and outcome. J. Perinat Med. 2018: 46(5): 465-88. doi: 10.1515/jpm-2017-0027.
- Зорина В.Н., Зорин Н.А. Белковые компоненты врожденного иммунитета в защите от патогенной инвазии. Журнал МЭИ. 2013; 3: 111-7.
- De Serres F., Blanco I. Role of alpha-1 antitrypsin in human health anddisease. J. Intern Med. 2014; 276(4): 311-35. doi: 10.1111/joim.12239.
- Azizia M.M., Irvine L.,M., Coker M. The role of C-reactive protein in modern obstetric and gynecological practice. Acta Obst Gynec Scand. 2006; 85(4): 394-401.
- Larsson A(1), Palm M., Hansson LO, Basu S., Axelsson O. Reference values for alpha1-acid glycoprotein, alpha1-antitrypsin, albumin,haptoglobin, C- reactive protein, IgA, IgG and IgM during pregnancy. Acta Obstet Gynecol Scand. 2008; 87(10):1084-8. doi: 10.1080/00016340802428146.
- Hsu T.Y., Hsieh T.T., Yang K.D., Tsai C.C., Ou C.Y., Cheng B.H., Wong Y.H., Hung H.N., Chou A.K., Hsiao C.C., Lin H. Proteomic profiling reveals a1-antitrypsin, a1-microglobulin, and clusterin as preeclampsia-related serum proteins in pregnant women. Taiwan J. Obstet Gynecol. 2015; 54(5): 499-504. doi: 10.1016/j.tjog.2014.01.007.
- Catarino C., Santos-Silva A., Belo L., Rocha-Pereira P., Rocha S., Patricio B., Quintanilha A., Rebelo I. Inflammatory disturbances in preeclampsia: relationship between maternal and umbilical cord blood. J. Pregnancy. 2012; 2012: 684384. doi: 10.1155/2012/684384.
- Baron J., Sheiner E., Abecassis A., Ashkenazi E., Shahaf G., Salem S.Y., Madar T., Twina G., Wiznitzer A., Holcberg G., Lewis E.C. a1-antitrypsin insufficiency is a possible contributor to preterm premature rupture of membranes. J. Matern Fetal Neonatal Med. 2012; 25(7): 934-7. doi: 10.3109/14767058.
- Izumi-Yoneda N., Toda A., Okabe M., Koike C., Takashima S., Yoshida, T., Konishi I., Saito S., Nikaido T. Alpha 1 antitrypsin activity is decreased in human amnion in premature rupture of the fetal membranes. Mol Hum Reprod. 2009; (1): 49-57. doi: 10.1093/molehr/gan071
Supplementary files
