Assessment of outcomes of embryo cryotransfer depending on the transcriptional profile of endometrial genes during the implantation window
- Authors: Miroshkina M.I1, Korneeva I.E1, Burmenskaya O.A1, Mishina N.D1
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Affiliations:
- FSBI «National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov» Ministry or Healthcare of Russian Federation
- Issue: No 4 (2020)
- Pages: 133-139
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/248920
- DOI: https://doi.org/10.18565/aig.2020.4.133-139
- ID: 248920
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Open Access
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Abstract
Objective. To assess the outcomes of assisted reproductive technologies (ART) depending on molecular genetic profiles of differentially expressed endometrial genes. Materials and methods. The study included 104 patients with tubal and peritoneal factor infertility who were performedpippelle endometrial biopsy in the natural cycle during the implantation window before embryo transfer. Histological and molecular genetic analysis of genes in endometrial samples was conducted using RT-PCR method. Outcomes of ART programs were analyzed afterfrozen-thawed embryo transfer into the uterine cavity. Results. The revealed association between the outcomes of ART programs and endometrial expression profile made it possible to determine additional intermediate stages of secretory endometrial development. Conclusion. The study of expression profiles of mRNA genes LIF, GPX3, AQP3, NDRG1, GNLY, IMPA2, PAEP, IGFBP1, HABP2, DPP4, TAGLN, IL15, POSTN, HLA-DOB, MSX1 allows for identifying the most favorable period for embryo cryotransfer.
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About the authors
M. I Miroshkina
FSBI «National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov» Ministry or Healthcare of Russian Federation
Email: maria_mir18@mail.ru
Postgraduate student of SEC for ART named after F. Paulsen 4 Oparin str, 117997, Moscow, Russia
I. E Korneeva
FSBI «National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov» Ministry or Healthcare of Russian Federation
Email: i_korneeva@oparina4.ru
PhD, Docent, Head of SEC for ART named after F. Paulsen 4 Oparin str, 117997, Moscow, Russia
O. A Burmenskaya
FSBI «National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov» Ministry or Healthcare of Russian Federation
Email: o_bourmenskaya@oparina4.ru
SD, Head of the Laboratory of Oncological Genetics 4 Oparin str, 117997, Moscow, Russia
N. D Mishina
FSBI «National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov» Ministry or Healthcare of Russian Federation
Email: mis7ha@gmail.com
junior researcher, Laboratory of genomic data analysis 4 Oparin str, 117997, Moscow, Russia
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