Expression of ephrin receptor A3 in glandular epithelial cells of uterine endometrium in patients with endometriosis and endometrial cancer


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Abstract

Objective: Evaluation of ephrin receptor A3 (EphA3) expression in glandular epithelial cells in uterine endometrium in women without endometrial pathology, with endometriosis and endometrial cancer (EC). Materials and methods: The study included 46 patients of reproductive age who underwent treatment in National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Health of Russia in the period from 2020 to 2021. The patients were divided into four groups: group 1 comprised 20 women with deep infiltrating endometriosis (DIE), group 2 comprised 21 women with peritoneal endometriosis (PE), group 3 consisted of 6 women with EC, and group 4 (control group) consisted of 9 women without endometriosis, who underwent surgery for tubal and peritoneal factors infertility, and the results of histological tests of these women showed no pathological changes in the endometrium. Additionally, the groups were divided into subgroups depending on the stage of the menstrual cycle prior to surgery. Semi-quantitative evaluation of EphA3 expression in glandular epithelial cells of uterine endometrium was performed by immunohistochemistry, using rabbit polyclonal antibodies (ab126261) and open-source image analysis software platform ImageJ. Results: Immunohistochemical examination showed that EphA3 expression in glandular epithlelial cells of normal endometrium and eutopic endometrium both in PE and DIE is significantly higher in the secretory phase versus the proliferative phase. Significantly high EphA3 expression in glandular epithelial cells of ectopic endometrium versus regular expression was detected in both phases of the cycle, in PE and DIE. There was no significant difference between EphA3 expression in glandular epithelial cells affected by endometrial cancer and in normal endometrium. Conclusion: The results of this study showed that EphA3 is overexpressed in ectopic endometrium, in PE and DIE, but not in EC.

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About the authors

Vladimir D. Chuprynin

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_chuprynin@oparina4.ru
PhD, Head of the Surgical Department

Shakhnoza K. Muftaydinova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

graduate student

Daria N. Senina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: n_buralkina@oparina4.ru
graduate student

Nafisa M. Fayzullina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Ph.D., Senior Researcher at the 1st Pathology Department

Alexandra V. Asaturova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_asaturova@oparina4.ru
Dr. Med. Sci., Head of the 1st Pathology Department

Natalya A. Buralkina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: n_buralkina@oparina4.ru
Dr. Med. Sci., Senior Researcher at Surgical Department

Leonid Z. Fayzullin

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: l_faizullin@oparina4.ru
PhD, Leading Researcher at the Laboratory of Molecular Genetic Methods

Dmitry L. Ovodenko

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: d_ovodenko@oparina4.ru
Dr. Med. Sci., Head of the Department of Clinical Work, Oncologist at the Department of Innovative Oncology and Gynecology

Andrey V. Kozachenko

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_kozachenko@oparina4.ru
Dr. Med. Sci., Leading Researcher

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