Modulation of the interaction between NK-cells and trophoblast by intravenous immunoglobulin


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Abstract

Objective: To investigate the effect of IVIG on the cytotoxicity of NK cells against target cells of K-562 line and trophoblast cells of JEG-3 line. Materials and methods: This study analyzed the cytotoxicity of NK-92 cells against K-562 cells and JEG-3 trophoblast cells in the presence of IVIG and that of peripheral blood NK cells in the mononuclear fraction against JEG-3 cells in the presence of IVIG. Mononuclear cells were obtained from the peripheral blood of healthy nonpregnant donors (group 1, n=10) and healthy nonpregnant fertile women (group 2, n=12). Results: Cell death of the JEG-3 line in the presence of mononuclear cells from Group 1 and IVIG at concentrations of 12 and 6 mg/mL was lower than that without IVIG (p<0.01 and p<0.05, respectively). Cell death of the JEG-3 line in the presence of mononuclear cells from group 2 and IVIG at concentrations of 12, 6, and 1.5 mg/ml was lower than that without IVIG (p<0.001, p<0.001, and p<0.01, respectively). Conclusion: Contact interaction between trophoblast cells and IVIG is associated with lower cytotoxicity of NK cells against trophoblast cells. More studies are needed to predict the effectiveness of IVIG therapy in patients with reproductive disorders.

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About the authors

Valentina A. Mikhailova

D.O. Ott Research Institute for Obstetrics, Gynecology and Reproductology

Senior Researcher at the Laboratory of Intercellular Interactions, Department of Immunology and Intercellular Interactions

Alina A. Davydova

D.O. Ott Research Institute for Obstetrics, Gynecology and Reproductology

Junior Researcher at the Laboratory of Intercellular Interactions, Department of Immunology and Intercellular Interactions

Dmitriy O. Bazhenov

D.O. Ott Research Institute for Obstetrics, Gynecology and Reproductology

Junior Researcher at the Laboratory of Intercellular Interactions, Department of Immunology and Intercellular Interactions

Anastasia A. Kovaleva

D.O. Ott Research Institute for Obstetrics, Gynecology and Reproductology

Laboratory Technician at the Laboratory of Intercellular Interactions, Department of Immunology and Intercellular Interactions

Valeriya A. Zagaynova

D.O. Ott Research Institute for Obstetrics, Gynecology and Reproductology

Junior Researcher, obstetrician-gynecologist at the Department of Assisted Reproductive Technologies

Igor Yu. Kogan

D.O. Ott Research Institute for Obstetrics, Gynecology and Reproductology

Corresponding Member of RAS, Dr. Med. Sci., Professor, Director

Olesya N. Bespalova

D.O. Ott Research Institute for Obstetrics, Gynecology and Reproductology

Dr. Med. Sci., Deputy Director

Alexandr M. Gzgzyan

D.O. Ott Research Institute for Obstetrics, Gynecology and Reproductology

Dr. Med. Sci, Head of the Department of Assisted Reproductive Technologies

Dmitry I. Sokolov

D.O. Ott Research Institute for Obstetrics, Gynecology and Reproductology

Dr. Bio. Sci., Head of the Laboratory of Intercellular Interactions, Department of Immunology and Intercellular Interactions

Sergey A. Selkov

D.O. Ott Research Institute for Obstetrics, Gynecology and Reproductology

Merited Scolar of the Russian Federation, Professor, Head of the Department of Immunology and Intercellular Interactions

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