Association of cumulus cell gene expression with embryological indicators in assisted reproductive technology programs


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Objective. To search for molecular genetic markers to assess the quality of oocytes and embryos with a high potential for development in order to enhance the efficiency of infertility treatment using assisted reproductive technologies. Subjects and methods. The investigation analyzed 89 cumulus cell samples from 39 patients who had undergone an in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) program. In terms of their quality, the obtained embryos were divided into 3 groups according to morphological quality assessment: 1) good-quality embryos (51 samples); 2) satisfactory-quality embryos (n = 19); 3) poor-quality embryos (n = 19). Real-time RT-PCR was used to estimate the cumulus cell mRNA expression in 7 genes: hyaluronan synthase 2 (HAS2), prostaglandin synthase 2 (PTFS2), gremlin 1 (GREM1), versican (VCAN), amphiregulin (AREG), inositol-triphosphate 3-kinase A (ITPKA), and activated leukocyte cell adhesion molecules (ALCAM or CD166). Results. A relationship was found between the expression levels of the CD 166 (ALCAM) gene and the indicators of embryo quality. This relationship was revealed when comparing good- and poor-quality embryos (p = 0.008). At the same time, there were no statistically significant differences between the quality of transferred embryos and the rate of clinical pregnancy (p = 0.856). Conclusion. The ALCAM gene may be a potential predictor for assessing the quality of embryos according to the morphological assessment criteria. Further investigation of the cumulus cell gene expression will assist in identifying potential biomarkers to assess the quality of oocytes and embryos and thus it will be able to further optimize the choice of transferred embryos and so to enhance the efficiency of IVF programs as a whole.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Natalia Safronova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: safrochik900@bk.ru
M.D., postgraduate of the department of assisted reproductive technology in infertility treatment

Elena Kalinina

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
M.D, Ph.D, The Head of the department of assisted reproductive technology in infertility treatment

Andrew Donnikov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
PhD, Senior Researcher of molecular-genetical laboratory

Olga Burmenskaya

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: o_bourmenskaya@oparina4.ru
Ph.D., Researcher of molecular-genetical laboratory

Natalia Makarova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: np.makarova@gmail.com
PhD, Researcher of the department of assisted reproductive technology in infertility treatment

Anastasia Zobova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: zoana20ll@gmail.com
PhD, Researcher of the department of assisted reproductive technology in infertility treatment

Kamila Alieva

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: k_alieva@oparina4.ru
PhD, Researcher of the department of assisted reproductive technology in infertility treatment

Victoria Gorshinova

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: chiasma@mail.ru
M.D., postgraduate of the department of assisted reproductive technology in infertility treatment

Dmitrii Trofimov

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: d_trofimov@oparina4.ru
Ph.D., The Head of molecular-genetical laboratory

Gennadii Sukhikh

Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

MD, PhD, Director

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