Role of mitochondrial DAMPs in the manifestation of hypertensive complications of pregnancy
- Autores: Skovorodina T.V.1, Vishnyakova P.A.1, Tsvirkun D.V.1, Shmakov R.G.1, Vysokikh M.Y.1
-
Afiliações:
- Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
- Edição: Nº 12 (2017)
- Páginas: 84-88
- Seção: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/248071
- DOI: https://doi.org/10.18565/aig.2017.12.84-88
- ID: 248071
Citar
Texto integral
Acesso aberto
Acesso está concedido
Acesso é pago ou somente para assinantes
Resumo
Objective. To comparatively analyze the level of the mitochondrial proteins TFAM, OPA1, and VDAC1 as possible damage-associated molecular patterns (DAMPs) in the microvesicular fraction from the plasma of pregnant women with preeclampsia (PE), chronic/gestational hypertension and that of healthy pregnant women. Material and methods. The microvesicular fraction was obtained from the plasma of patients by differential centrifugation. The levels of TFAM, OPA1, and VDAC1 were determined by chemiluminescent western blotting. Results. The severe PE group was found to have a significant increase in the expression level of ОРА1L and pre-TFAM proteins and a correlation with the level of proteinuria as an important diagnostic marker for PE. Conclusion. The ОРА1-L and pre-TFAM proteins can lay claim to being PE-associated DAMPs. It is necessary to perform further investigations of the association between certain DAMPs and the development of PE.
Palavras-chave
Texto integral
Sobre autores
Tatyana Skovorodina
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
Email: t_skovorodina@oparina4.ru
Ph.D
Polina Vishnyakova
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
Email: p_vishnyakova@oparina4.ru
researcher at the Laboratory of mitochondrial medicine
Darya Tsvirkun
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
Email: darunyat@gmail.com
PhD
Roman Shmakov
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
Email: r_shmakov@oparina4.ru
chief physician
Mikhail Vysokikh
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
Email: m_vysokikh@oparina4.ru
PhD, Head of mitochondrial medicine research group
Bibliografia
- Основные показатели здоровья матери и ребенка, деятельность службы охраны детства и родовспоможения в Российской Федерации. М.: ФГБУ “ЦНИИОИЗ” Минздрава Российской Федерации; 2015. 175с. [The main indicators of maternal and child health, the activities of the child welfare service and obstetrics in the Russian Federation. Moscow: FGBU “TSNIIOIZ” of the Ministry of Health of the Russian Federation; 2015. 175p. (in Russian)]
- Ходжаева З.С., Коган Е.А., Клименченко Н.И., Акатьева А.С., Сафонова А.Д., Холин А.М., Вавина О.В., Сухих Г.Т. Клинико-патогенетические особенности ранней и поздней преэклампсии. Акушерство и гинекология. 2015; 1: 12-7. [Khodzhaeva Z.S., Kogan E.A., Klimenchenko N.I., Akatyeva A.S., Kholin A.M., Vavina O.V., Sukhikh G.T. Clinical and pathogenetic features of early and late preeclampsia. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2015; (1): 12-7. (in Russian)]
- De Oliveira L., Peraçoli J.C., Peraçoli M.T., Korkes H., Zampieri G., Moron A.F., Sass N.sFlt-1/PlGF ratio as a prognostic marker of adverse outcomes in women with early-onset preeclampsia. Pregnancy Hypertens. 2013; 3(3): 191-5.
- McCarthy C.M., Kenny L.C. Immunostimulatory role of mitochondrial DAMPs: alarming for pre-eclampsia? Am. J. Reprod. Immunol. 2016; 76(5): 341-7.
- Fülöp L., Szanda G., Enyedi B., Várnai P., Spät A. The effect of OPA1 on mitochondrial Ca2+ signaling. PLoS One. 2011; 6 (9): e25199.
- Landes T., Leroy I., Bertholet A., Diot A., Khosrobakhsh F., Daloyau M. et al. OPA1 (dys)functions. Semin. Cell Dev. Biol. 2010; 21(6): 593-8.
- Elachouri G., Vidoni S., Zanna C., Pattyn A., Boukhaddaoui H., Gaget K. et al. OPA1 links human mitochondrial genome maintenance to mtDNA replication and distribution. Genome Res. 2011; 21(1): 12-20.
- Benard G., Karbowski M. Mitochondrial fusion and division: Regulation and role in cell viability. Semin. Cell Dev. Biol. 2009; 20(3): 365-74.
- Вишнякова П.А., Тарасова Н.В., Володина М.А., Марей М.В., Ходжаева З.С., Кан Н.Е., Высоких М.Ю. Эпителиально-мезенхимальный переход в плаценте при преэклампсии. Акушерство и гинекология. 2016; 12: 53-7. http://dx.doi.org/10.18565/aig.2016.12.53-7 [Vishnyakova P.A., Tarasova N.V., Volodina M.A., Marei M.V., Khodzhaeva Z.S., Kan N.E., Vysokikh M.Yu. Placental epithelial-mesenchymal transition in preeclampsia. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2016; (12): 53-7. (in Russian) https:// dx.doi.org/10.18565/aig.2016.12.53-57]
- He J., Lu Y., Xia H., Liang Y., Wang X., Bao W. et al. Circulating mitochondrial DAMPs are not effective inducers of proteinuria and kidney injury in rodents. PLoS One. 2015; 10(4): e0124469.
Arquivos suplementares
