Role of mitochondrial DAMPs in the manifestation of hypertensive complications of pregnancy
- Authors: Skovorodina T.V.1, Vishnyakova P.A.1, Tsvirkun D.V.1, Shmakov R.G.1, Vysokikh M.Y.1
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Affiliations:
- Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
- Issue: No 12 (2017)
- Pages: 84-88
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/248071
- DOI: https://doi.org/10.18565/aig.2017.12.84-88
- ID: 248071
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Open Access
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Abstract
Objective. To comparatively analyze the level of the mitochondrial proteins TFAM, OPA1, and VDAC1 as possible damage-associated molecular patterns (DAMPs) in the microvesicular fraction from the plasma of pregnant women with preeclampsia (PE), chronic/gestational hypertension and that of healthy pregnant women. Material and methods. The microvesicular fraction was obtained from the plasma of patients by differential centrifugation. The levels of TFAM, OPA1, and VDAC1 were determined by chemiluminescent western blotting. Results. The severe PE group was found to have a significant increase in the expression level of ОРА1L and pre-TFAM proteins and a correlation with the level of proteinuria as an important diagnostic marker for PE. Conclusion. The ОРА1-L and pre-TFAM proteins can lay claim to being PE-associated DAMPs. It is necessary to perform further investigations of the association between certain DAMPs and the development of PE.
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About the authors
Tatyana Vyacheslavovna Skovorodina
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
Email: t_skovorodina@oparina4.ru
Ph.D
Polina A. Vishnyakova
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
Email: p_vishnyakova@oparina4.ru
researcher at the Laboratory of mitochondrial medicine
Darya Viktorovna Tsvirkun
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
Email: darunyat@gmail.com
PhD
Roman G. Shmakov
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
Email: r_shmakov@oparina4.ru
chief physician
Mikhail Yu. Vysokikh
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russia
Email: m_vysokikh@oparina4.ru
PhD, Head of mitochondrial medicine research group
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