Features of the composition of peripheral blood lymphocyte subpopulations with cytotoxic, killer, and regulatory functions in women with recurrent implantation failures in ART programs


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Objective. To analyze the content of peripheral blood lymphocyte subpopulations with cytotoxic, killer, and regulatory functions in female patients with recurrent implantation failures in assisted reproductive technology programs. Subjects and methods. The investigation enrolled 48 patients with recurrent implantation failures in an IVF program and 26 fertile women with their last child aged less than 2 years. Results. There was a reduction in T lymphocytes (CD3+) and T lymphocytes with cytotoxic function (CD3+CD8+) and an increase in innate immunity cells with both cytotoxic (CD3-CD8+) and killer (CD56+, CD16+, CD56,16+) functions. The group patients showed a significant rise in gd+Т cell levels in a cryo-cycle (p = 0.005). Conclusion. In patients with recurrent implantation failures, the composition of lymphocyte subpopulations is characterized by a decrease in T lymphocytes (CD3+) and Tlymphocytes with cytotoxic function (CD3+CD8+) and by an increase in innate immunity cells with both cytotoxic and killer functions.

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作者简介

Tatiana Amyan

Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov

Email: amyantanya@rambler.ru
a graduate student of the 1st year of study department of conservation and restoration of reproductive function

Lubov Krechetova

Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov

Email: k_l_v_@mail.ru
PhD, Senior Research Fellow, Head of the Laboratory Laboratory of Clinical Immunology

Svetlana Perminova

Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov

Email: perisvet@list.ru
MD, a leading researcher department of conservation and restoration of reproductive function

Valentina Vtorushina

Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov

Email: vtorushina@inbox.ru
PhD, doctor of clinical laboratory diagnostics

参考

  1. Coughlan C., Ledger W., Wang Q., Liu F., Demirol A., Gurgan T. et al. Recurrent implantation failure: definition and management. Reprod. Biomed. Online. 2014; 28(1): 14-38.
  2. Liang P.Y., Yin B., Cai J., Hu X.D., Song C., Wu T.H. et al. The proinflammatory and anti-inflammatory cytokine profile in peripheral blood of women with recurrent implantation failure. Am. J. Reprod. Immunol. 2015; 73(1): 12-21.
  3. Сухих Г.Т., Ванько Л.В. Иммунные факторы в этиологии и патогенезе осложнений беременности. Акушерство и гинекология. 2012; 1: 128-36. [Sukhikh G.X., Vanko L.V. Immune factors in the etiology and pathogenesis of pregnancy complications. Akusherstvo i Ginekologiya/ Obstetrics and Gynecology. 2012; (1): 128-36. (in Russian)]
  4. Степанова Е.О., Николаева М.А., Бабаян А.А., Смольникова В.Ю., Ванько Л.В., Кречетова Л.В. Роль регуляторных клеток в формировании иммунной толерантности при беременности. Акушерство и гинекология. 2013; 2: 24-8. [Stepanova E.O., Nikolayeva M.A., Babayan A.A., Smolnikova V.Yu., Vanko L.V., Krechetova L.V. Role of regulatory Т cells in the development of immune tolerance in pregnancy. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2013; (2): 24-8. (in Russian)]
  5. Chaouat G. Inflammation, NK cells and implantation: friend and foe (the good, the bad and the ugly?): replacing placental viviparity in an evolutionary perspective. J. Reprod. Immunol. 2013; 97(1): 2-13.
  6. Williams Z. Inducing tolerance to pregnancy. N Engl J. Med. 2012; 367(12): 1159-61.
  7. Zenclussen A.C. Regulatory T cells in pregnancy. Springer Semin. Immunopathol. 2006; 28: 31-9.
  8. Yu N., Li X., Song W., Li D., Yu D., Zeng X. et al. OT4(+)CD25(+) CD127(low/-) T cells: a more specific Treg population in human peripheral blood. Inflammation. 2012; 35(6): 1773-80.
  9. Saito S., Nakashima A., Shima T., Ito M. Th1/Th2/Th17 and regulatory T-cell paradigm in pregnancy. Am. J. Reprod. Immunol. 2010; 63(6): 601-10.
  10. Aluvihare V.R., Kallikourdis M., Betz A.G. Regulatory T cells mediate maternal tolerance to the fetus. Nat. Immunol. 2004; 5(3): 266-71.
  11. Arruvito L., Sanz M., Banham A.H., Fainboim L. Expansion of CD4+CD25+ and FOXP3+ regulatory T cells during the follicular phase of the menstrual cycle: implications for human reproduction. J. Immunol. 2007; 178(4): 2572-8.
  12. Nikahava H., Kato T., Tawara I., Ikeda H., Kuribayashi K., Shiky H. et al. IFN-y controls the generation/activation of CD4+CD25+ regulatory T cell in antitumor immune response. J. Immunol. 2005; 175(7): 4433-40.
  13. Fainboim L., Arruvito L., Sanz M., Alison H. Expansion of CD4+CD25+ and FOXP3+ regulatory T cells during the follicular phase of the menstrual cycle: implications for human reproduction. J. Immunol. 2017; 178(4): 2572-8.
  14. Vrekoussis T., Kalantaridou S.N., Mastorakos G., Zoumakis E., Makrigiannakis A., Syrrou M. et al. The role of stress in female reproduction and pregnancy: an update. Ann. N. Y. Acad. Sci. 2010; 1205: 69-75.

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