Composition of biologically active compounds of three-part beggarticks herb

Introduction. Three-part beggarticks herb is a pharmacopoeial medicinal plant raw material that has found wide application in clinical practice as an external remedy for skin manifestations of exudative diathesis, pyoderma and as an internal remedy for acute respiratory viral infections. In Russian Federation, anti-inflammatory medicinal herbal preparations based on it (herb crushed for the preparation of infusion, herb powder in filter bags) are produced and sold. Despite the high degree of scientific knowledge of this medicinal raw material, the improvement of approaches to standardization remains an urgent issue. Objective: identification of the biologically active compounds composition of the industrially produced three-part beggarticks herb. Material and methods. Biologically active compounds were analyzed using ultra-high-performance liquid chromatography with photodiode array and tandem quadrupole mass-selective detection in the gradient elution mode. Extraction was carried out with 70% aqueous methanol in an ultrasonic bath. Results. The presence of the main groups of biologically active compounds characteristic for three-part beggarticks herb was confirmed. 18 compounds were identified, including sulfuretin, okaniin, luteolin, glycosides, hydroxycinnamic acids, polyacetylenes. Conclusion. The proper quality of medicinal herbal raw materials and the drugs based on them can only be guaranteed if the composition of marker compounds is reliably determined, which makes it possible to confirm its identity. In this regard, a detailed analysis of the phytochemical composition, performed in this study, is a prerequisite for scientifically sound improvement of regulatory documentation. Polyacetylenes are a rather rare and specific group of biologically active compounds in medicinal plant raw materials. In order to standardize the three-part beggarticks herb, it is also recommended to determine them along with other compounds of the polyphenolic complex (flavonoids, hydroxycinnamic acids) and polysaccharides.