Qualitative and quantitative analysis of the main psoralen derivatives in the juice of Sosnowsky's hogweed

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Introduction. Sosnovsky's hogweed is a dangerous weed and has a pronounced photosensitizing effect. The photosensitizing effect of hogweed is conditioned to the presence of furanocoumarins in all parts of the plant. Due to its large biomass, fast growth rate, and unpretentiousness to natural conditions, it is promising as a medicinal plant material suitable for the production of photosensitizers for PUVA therapy. Objective: to conduct a qualitative and quantitative determination of psoralen derivatives in the juice of Sosnowsky's hogweed. Material and methods. For the study, wild-growing samples of raw materials were used. Raw materials of Sosnowsky's hogweed were harvested during the flowering period of the plant. To study the content of furanocoumarins in hogweed Sosnowski, juice obtained from the aerial part of the plant was used. The juice was subjected to extraction with chloroform. Purification and separation of the extract were carried out using gradient column chromatography. The extract fractions were subjected to qualitative and quantitative analysis using thin layer chromatography, spectrophotometry, NMR spectroscopy and HPLC. Results. 6 main fractions were obtained during column chromatography of Sosnowsky's hogweed juice extract. The fraction of the extract richest in furanocoumarins was obtained. According to thin-layer chromatography, spectrophotometry and NMR-spectroscopy, the main furanocoumarins of Sosnowsky's hogweed juice are psoralen derivatives. According to HPLC data, the content of 8-methoxypsoralen in the juice of Sosnowsky's hogweed was 1.332 g/l, and 5-methoxypsoralen was 0.034 g/l. Conclusion. Raw materials of Sosnowsky's hogweed can be a source of furanocoumarins for the needs of pharmacy, and the methods of analysis presented in the study can form the basis of its standardization. The Sosnowsky's hogweed, having become a wild-growing source of medicinal raw materials, can be subjected to regular harvesting, which can reduce the area of its distribution.

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作者简介

Valentin Ageev

Federal State Budgetary Educational Institution of Higher Education «National Research Ogarev Mordovia State University»

编辑信件的主要联系方式.
Email: valeageev@yandex.ru
junior researcher, laboratory of pharmacokinetics and targeted pharmacotherapy

Vasilisa Shlyapkina

Federal State Budgetary Educational Institution of Higher Education «National Research Ogarev Mordovia State University»

Email: shlyapkina.98@mail.ru
PhD student of the department of pharmacology and clinical pharmacology with the course of pharmaceutical technology

Oleg Kulikov

Federal State Budgetary Educational Institution of Higher Education «National Research Ogarev Mordovia State University»

Email: oleg-kulikov-84@mail.ru
Doctor of Medicine, associate professor of the department of pharmacology and clinical pharmacology with the course of pharmaceutical technology

Andrey Zaborovskiy

Federal State Budgetary Educational Institution of Higher Education "A.I. Yevdokimov Moscow State University of Medicine and Dentistry" of the Ministry of Healthcare of the Russian Federation

Email: azabor@mail.ru
Doctor of Medicine, associate professor, Head of the department of pharmacology

Larisa Tararina

Federal State Budgetary Educational Institution of Higher Education "A.I. Yevdokimov Moscow State University of Medicine and Dentistry" of the Ministry of Healthcare of the Russian Federation

Email: 79104906528@yandex.ru
senior lecturer of department of pharmacology

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2. Fig. 1. Thin layer chromatography on Sorbfil silica gel plates (detection at 360 nm)

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3. Fig. 2. Absorption spectra in the UV/Vis ranges of extraction fractions of Sosnovsky's hogweed and 5-MOP, 8-MOP. The spectrum of samples was taken in acetonitrile

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4. Fig. 3. HPLC chromatogram of Sosnovsky's hogweed juice fractions and analytical standards 5-MOP and 8-MOP

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5. Fig. 4. 1H NMR spectrum of F-2.2

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6. Fig. 5.13C NMR spectrum of F-2.2

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