Identification of phenolic compounds in Artemisia scoparia

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详细

Introduction. Artemisia scoparia herba is a valuable source of biologically active compounds. Due to its rich chemical composition, the raw material has wide pharmacological effects.

Purpose of the study. Identification of some phenolic compounds and the quantitative determination of scoparone, scopoletin, umbelliferonein Artemisia scoparia herba by high performance liquid chromatography (HPLC).

Material and methods. The investigated object is Artemisia scoparia herba, collected during the flowering phase in the Nikitsky Botanical Garden in 2018–2019. Qualitative determination of phenolic compounds was provided by HPLC with mass-spectrometry detection (HPLC-MS).

Results. The following phenolic compounds were identified by HPLC-MS: scopoletin, rutin, hyperoside, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, 1,4-dicaffeoylquinic acid, luteolin.

Conclusion. The possibility of qualitative and quantitative determination of phenolic compounds from plant raw materials by HPLC is shown.

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作者简介

Emma Ayrapetyan

Pyatigorsk Medical and Pharmaceutical Institute is a branch of the Volgograd State Medical University of the Ministry of Health

编辑信件的主要联系方式.
Email: airapetyan0505@mail.ru
ORCID iD: 0000-0002-4435-7074

teacher of the Department of morphology

俄罗斯联邦, Kalinin Ave., 11, Pyatigorsk, 357532

Mikhail Larsky

Pyatigorsk Medical and Pharmaceutical Institute is a branch of the Volgograd State Medical University of the Ministry of Health

Email: m.v.larsky@pmedpharm.ru
ORCID iD: 0000-0002-4406-7165

Associate Professor of the Department of Pharmaceutical Chemistry

俄罗斯联邦, Kalinin Ave., 11, Pyatigorsk, 357532

Dmitry Konovalov

Pyatigorsk Medical and Pharmaceutical Institute is a branch of the Volgograd State Medical University of the Ministry of Health

Email: d.a.konovalov@pmedpharm.ru
ORCID iD: 0000-0002-0960-6127

Head of the Department of Pharmacognosy, Botany and Technology of Phytopreparations

俄罗斯联邦, Kalinin Ave., 11, Pyatigorsk, 357532

参考

  1. Айрапетян Э.Э., Леонова В.Н., Коновалов Д.А. Разработка методики количественного определения суммы флавоноидов в полыни метельчатой траве.Человек и его здоровье. 2022; 25: 105–12. [Hayrapetyan E.E., Leonova V.N., Konovalov D.A. Development of a method for quantitative determination of the sum of flavonoids in Artemisia scoparia. Man and his health. 2022; 25: 105–12. doi: 10.21626/vestnik/2022-2/11 (in Russian)].
  2. Boakye Y.D. et al. Artemisia scoparia: A review on traditional uses, phytochemistry and pharmacological properties. International J. of Chemical and Biochemical Science. 2017; 12: 92–7.
  3. Коновалов Д.А. Природные полиацетиленовые соединения. Фармация и фармакология. 2014; 4 (5): 23–48. [Konovalov D.A. Natural polyacetylene compounds. Pharmacy and pharmacology. 2014; 4 (5): 23–48 (in Russian)].
  4. Pozdnyakov D.I. Ayrapetyan E.E., Konovalov D.A. The study of the anti-inflammatory activity of a stomatological gel based on an extract of Artemisia scoparia Waldst. et Kit. Journal of Research in Pharmacy. 2022; 26 (1): 189–97.
  5. Liu Y. P. Research progress on pharmacological effect of Artemisiae Scopariae Herba. Chinese Traditional and Herbal Drugs. 2019; 2235–41.
  6. Clifford M.N., Knight S., Kuhnert N. Discriminating between the six isomers of dicaffeoylquinic acid by LC-MS(n) J. Agric. Food Chem. 2005; 53: 3821−32.
  7. Vuković, N. L. et al. The investigation of bioactive secondary metabolites of the methanol extract of Eryngium amethystinum. Kragujevac Journal of Science. 2018; 40: 113–29.
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2. Fig. 1. Chromatogram of alcohol extraction of Artemisia scoparia herba (UV detection, 254 nm)

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3. Fig. 2. An approximate chromatogram of the extraction of Artemisia scoparia

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