Email this article Personalized approach to assessing mRNA expression of histidine-rich glycoprotein and immunohistochemical markers in diseases of the breast
- Authors: Autenshlyus A.I.1,2, Golovanova A.V.1, Studenikina A.A.1, Brusentsov I.I.3, Proskura A.V.2, Zhurakovskiy I.P.1, Arkhipov S.A.1,2, Sidorov S.V.4, Vavilin V.A.2, Lyakhovich V.V.2
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Affiliations:
- Novosibirsk State Medical University
- Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine
- Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
- Novosibirsk State University
- Issue: Vol 484, No 5 (2019)
- Pages: 624-628
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/12787
- DOI: https://doi.org/10.31857/S0869-56524845624-628
- ID: 12787
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Abstract
Biopsy material of patients with malignant and benign breast diseases was examined. HRG mRNA expression was detected in 70% of cases in biopsy material obtained from patients with nonspecific invasive carcinoma and in 66.7% of cases in biopsy material of patients with benign breast diseases. Immunohistochemical analysis revealed expression of collagen II, the beta-1 integrin, and E-cadherin – markers of epithelial–mesenchymal transition. The use of RT-qPCR combined with immunohistochemical study made it possible to identify atypical cells, which can be regarded as precancerous changes, in individual patients.
About the authors
A. I. Autenshlyus
Novosibirsk State Medical University; Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine
Author for correspondence.
Email: lpciip@211.ru
Russian Federation, Novosibirsk
A. V. Golovanova
Novosibirsk State Medical University
Email: lpciip@211.ru
Russian Federation, Novosibirsk
A. A. Studenikina
Novosibirsk State Medical University
Email: lpciip@211.ru
Russian Federation, Novosibirsk
I. I. Brusentsov
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences
Email: lpciip@211.ru
Russian Federation, Novosibirsk
A. V. Proskura
Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine
Email: lpciip@211.ru
Russian Federation, Novosibirsk
I. P. Zhurakovskiy
Novosibirsk State Medical University
Email: lpciip@211.ru
Russian Federation, Novosibirsk
S. A. Arkhipov
Novosibirsk State Medical University; Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine
Email: lpciip@211.ru
Russian Federation, Novosibirsk
S. V. Sidorov
Novosibirsk State University
Email: lpciip@211.ru
Russian Federation, Novosibirsk
V. A. Vavilin
Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine
Email: lpciip@211.ru
Russian Federation, Novosibirsk
V. V. Lyakhovich
Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine
Email: lpciip@211.ru
Academician of the RAS
Russian Federation, NovosibirskReferences
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