Personalized approach to assessing mRNA expression of histidine-rich glycoprotein and immunohistochemical markers in diseases of the breast

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Abstract


Biopsy material of patients with malignant and benign breast diseases was examined. HRG mRNA expression was detected in 70% of cases in biopsy material obtained from patients with nonspecific invasive carcinoma and in 66.7% of cases in biopsy material of patients with benign breast diseases. Immunohistochemical analysis revealed expression of collagen II, the beta-1 integrin, and E-cadherin – markers of epithelial–mesenchymal transition. The use of RT-qPCR combined with immunohistochemical study made it possible to identify atypical cells, which can be regarded as precancerous changes, in individual patients.


About the authors

A. I. Autenshlyus

Novosibirsk State Medical University; Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine

Author for correspondence.
Email: lpciip@211.ru

Russian Federation, Novosibirsk

A. V. Golovanova

Novosibirsk State Medical University

Email: lpciip@211.ru

Russian Federation, Novosibirsk

A. A. Studenikina

Novosibirsk State Medical University

Email: lpciip@211.ru

Russian Federation, Novosibirsk

I. I. Brusentsov

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences

Email: lpciip@211.ru

Russian Federation, Novosibirsk

A. V. Proskura

Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine

Email: lpciip@211.ru

Russian Federation, Novosibirsk

I. P. Zhurakovskiy

Novosibirsk State Medical University

Email: lpciip@211.ru

Russian Federation, Novosibirsk

S. A. Arkhipov

Novosibirsk State Medical University; Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine

Email: lpciip@211.ru

Russian Federation, Novosibirsk

S. V. Sidorov

Novosibirsk State University

Email: lpciip@211.ru

Russian Federation, Novosibirsk

V. A. Vavilin

Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine

Email: lpciip@211.ru

Russian Federation, Novosibirsk

V. V. Lyakhovich

Federal State Budgetary Scientific Institution Research Institute of Experimental and Clinical Medicine

Email: lpciip@211.ru

Russian Federation, Novosibirsk

Academician of the RAS

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