Email this article Principles for construction of bioluminescent enzyme biotests for analysis of complex media
- Authors: Kalyabina V.P.1,2, Esimbekova E.N.1,2, Torgashina I.G.1, Kopylova K.V.1, Kratasyuk V.A.1,2
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Affiliations:
- Siberian Federal University
- Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science
- Issue: Vol 485, No 2 (2019)
- Pages: 229-233
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/12858
- DOI: https://doi.org/10.31857/S0869-56524852229-233
- ID: 12858
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Abstract
We formulated the principles of designing bioluminescent enzyme tests for assessing the quality of complex media which consist in providing the maximum sensitivity to potentially toxic chemicals at a minimal impact of uncontaminated complex media. The developed principles served as a basis for designing a new bioluminescent method for an integrated rapid assessment of chemical safety of fruits and vegetables which is based on using the luminescent bacterium enzymes (NAD(P)H:FMN oxidoreductase and luciferase) as a test system.
About the authors
V. P. Kalyabina
Siberian Federal University;Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science
Author for correspondence.
Email: esimbekova@yandex.ru
Russian Federation, Krasnoyarsk;Krasnoyarsk
E. N. Esimbekova
Siberian Federal University;Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science
Email: esimbekova@yandex.ru
Russian Federation, Krasnoyarsk;Krasnoyarsk
I. G. Torgashina
Siberian Federal University
Email: esimbekova@yandex.ru
Russian Federation, Krasnoyarsk
K. V. Kopylova
Siberian Federal University
Email: esimbekova@yandex.ru
Russian Federation, Krasnoyarsk
V. A. Kratasyuk
Siberian Federal University;Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science
Email: esimbekova@yandex.ru
Russian Federation, Krasnoyarsk;Krasnoyarsk
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