Principles for construction of bioluminescent enzyme biotests for analysis of complex media

V. P. Kalyabina; Калябина В. П.; E. N. Esimbekova; Есимбекова Е. Н.; I. G. Torgashina; Торгашина И. Г.; K. V. Kopylova; Копылова К. В.; V. A. Kratasyuk; Кратасюк В. А.

doi:10.31857/S0869-56524852229-233

Principles for construction of bioluminescent enzyme biotests for analysis of complex media

Authors: Kalyabina V.P.¹^,2, Esimbekova E.N.¹^,2, Torgashina I.G.¹, Kopylova K.V.¹, Kratasyuk V.A.¹^,2
Affiliations:
1. Siberian Federal University
2. Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science
Issue: Vol 485, No 2 (2019)
Pages: 229-233
Section: Biochemistry, biophysics, molecular biology
URL: https://journals.eco-vector.com/0869-5652/article/view/12858
DOI: https://doi.org/10.31857/S0869-56524852229-233
ID: 12858

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Abstract

We formulated the principles of designing bioluminescent enzyme tests for assessing the quality of complex media which consist in providing the maximum sensitivity to potentially toxic chemicals at a minimal impact of uncontaminated complex media. The developed principles served as a basis for designing a new bioluminescent method for an integrated rapid assessment of chemical safety of fruits and vegetables which is based on using the luminescent bacterium enzymes (NAD(P)H:FMN oxidoreductase and luciferase) as a test system.

Keywords

bioluminescent method, complex media, food safety analysis, heavy metals, pesticides, luminous bacteria enzymes, enzyme tests

References

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Vol 489, No 6 (2019)

Principles for construction of bioluminescent enzyme biotests for analysis of complex media

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Abstract

Keywords

About the authors

V. P. Kalyabina

E. N. Esimbekova

I. G. Torgashina

K. V. Kopylova

V. A. Kratasyuk

References

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