The steroidogenic effect of a low-molecular agonist of luteinizing hormone receptor in the course of its administration to male rats

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Abstract

The thienopyrimidine derivative TP03 (a low molecular weight agonist of the luteinizing hormone receptor, LHR), when administered to male Wistar rats, stimulated the production of testosterone (“T”), elevated level of which was maintained for 7 days, and increased the expression of the LHR gene. At the same time, the steroidogenic effect of human chorionic gonadotropin (hCG), which significantly increased the “T” level on the first day of treatment, was further weakened, which was accompanied by a decrease in the expression of the LHR gene in the testes, indicating the development of resistance of Leydig cells to hCG. In the testes, the hCG treatment induced a compensatory increase in the expression of genes of the steroidogenic enzymes, such as the cytochrome P450scc and the dehydrogenase 3P-HSD. In the case of TP03 treatment this effect was absent.

About the authors

A. A. Bakhtyukov

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

Author for correspondence.
Email: alex_shpakov@list.ru
Russian Federation, 44, Thorez prospekt, St.-Petersburg, 194223

K. V. Derkach

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

Email: alex_shpakov@list.ru
Russian Federation, 44, Thorez prospekt, St.-Petersburg, 194223

D. V. Dar'in

Saint-Petersburg State University

Email: alex_shpakov@list.ru
Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034

A. O. Shpakov

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

Email: alex_shpakov@list.ru
Russian Federation, 44, Thorez prospekt, St.-Petersburg, 194223

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