Email this article Functional analysis of coilin in virus resistance and stress tolerance of potato Solanum tuberosum USING CRISPR/Cas9 editing
- Authors: Makhotenko A.V.1,2, Khromov A.V.1,2, Snigir E.A.1, Makarova S.S.1,2, Makarov V.V.1, Suprunova T.P.1, Kalinina N.O.1,2, Taliansky M.E.1,3
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Affiliations:
- Doka - Gene Technologies LLC
- Lomonosov Moscow State University
- Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Issue: Vol 484, No 6 (2019)
- Pages: 772-776
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/12903
- DOI: https://doi.org/10.31857/S0869-56524846772-776
- ID: 12903
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Abstract
The role of the nuclear protein coilin in the mechanisms of resistance of potato Solanum tuberosum cultivar Chicago to biotic and abiotic stresses was studied using the CRISPR/Cas9 technology. For the coilin gene editing, a complex consisting of the Cas9 endonuclease and a short guide RNA was immobilized on gold or chitosan microparticles and delivered into apical meristem cells by bioballistics or vacuum infiltration methods, respectively. Editing at least one allele of the coilin gene considerably increased the resistance of the edited lines to infection with the potato virus Y and their tolerance to salt and osmotic stress.
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About the authors
A. V. Makhotenko
Doka - Gene Technologies LLC; Lomonosov Moscow State University
Author for correspondence.
Email: kalinina@genebee.msu.ru
Russian Federation, Rogachevo, Moscow region; 1, Leninskie gory, Moscow, 119991
A. V. Khromov
Doka - Gene Technologies LLC; Lomonosov Moscow State University
Email: kalinina@genebee.msu.ru
Russian Federation, Rogachevo, Moscow region; 1, Leninskie gory, Moscow, 119991
E. A. Snigir
Doka - Gene Technologies LLC
Email: kalinina@genebee.msu.ru
Russian Federation, Rogachevo, Moscow region
S. S. Makarova
Doka - Gene Technologies LLC; Lomonosov Moscow State University
Email: kalinina@genebee.msu.ru
Russian Federation, Rogachevo, Moscow region; 1, Leninskie gory, Moscow, 119991
V. V. Makarov
Doka - Gene Technologies LLC
Email: kalinina@genebee.msu.ru
Russian Federation, Rogachevo, Moscow region
T. P. Suprunova
Doka - Gene Technologies LLC
Email: kalinina@genebee.msu.ru
Russian Federation, Rogachevo, Moscow region
N. O. Kalinina
Doka - Gene Technologies LLC; Lomonosov Moscow State University
Email: kalinina@genebee.msu.ru
Russian Federation, Rogachevo, Moscow region; 1, Leninskie gory, Moscow, 119991
M. E. Taliansky
Doka - Gene Technologies LLC; Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: kalinina@genebee.msu.ru
Russian Federation, Rogachevo, Moscow region; 16/10, Miklukho-Maklaya Street, Moscow, 117997
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