Email this article Optimization of heterological expression of insulin receptor-related receptor ectodomain
- Authors: Mozhaev A.A.1,2, Orsa A.N.1, Deyev I.E.1, Shvets V.I.3, Petrenko A.G.1
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Affiliations:
- Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Shubnikov Crystallography Institute of the Russian Academy of Sciences
- Moscow University of Technology (Institute of Fine Chemical Technologies)
- Issue: Vol 485, No 1 (2019)
- Pages: 110-123
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/12916
- DOI: https://doi.org/10.31857/S0869-56524851110-123
- ID: 12916
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Abstract
In this paper, we present an approach to optimize the heterologous expression of the receptor tyrosine kinase IRR, which further simplifies the purification of the IRR from the medium and increases the final yield. The approach proposed by us can find application in the biotechnological production of other large-scale recombinant proteins produced for medical purposes.
About the authors
A. A. Mozhaev
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Shubnikov Crystallography Institute of the Russian Academy of Sciences
Author for correspondence.
Email: a.a.mozhaev@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997; 59, Leninsky prospect, Moscow, 117333
A. N. Orsa
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: a.a.mozhaev@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997
I. E. Deyev
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: a.a.mozhaev@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997
V. I. Shvets
Moscow University of Technology (Institute of Fine Chemical Technologies)
Email: a.a.mozhaev@gmail.com
Academician of the RAS
Russian Federation, 86, Vernadsky avenue, Moscow, 119571A. G. Petrenko
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: a.a.mozhaev@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997
References
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