Optimization of heterological expression of insulin receptor-related receptor ectodomain

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Abstract

In this paper, we present an approach to optimize the heterologous expression of the receptor tyrosine kinase IRR, which further simplifies the purification of the IRR from the medium and increases the final yield. The approach proposed by us can find application in the biotechnological production of other large-scale recombinant proteins produced for medical purposes.

About the authors

A. A. Mozhaev

Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Shubnikov Crystallography Institute of the Russian Academy of Sciences

Author for correspondence.
Email: a.a.mozhaev@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997; 59, Leninsky prospect, Moscow, 117333

A. N. Orsa

Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: a.a.mozhaev@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997

I. E. Deyev

Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: a.a.mozhaev@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997

V. I. Shvets

Moscow University of Technology (Institute of Fine Chemical Technologies)

Email: a.a.mozhaev@gmail.com

Academician of the RAS

Russian Federation, 86, Vernadsky avenue, Moscow, 119571

A. G. Petrenko

Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: a.a.mozhaev@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997

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