Peroxide-induced oxidative modification of hemoglobin

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Abstract


The oxidative modification of human hemoglobin Hb treated with ydrogen peroxide was investigated. The method of mass spectrometry were detected oxidized amino acid residues of the hemoglobin molecule: αTrp14, αTyr24, αArg31, αMet32, αTyr42, αHis45, αHis72, αMet76, αPro77, αLys90, αCys104, αTyr140, βHis2, βTrp15, βTrp37, βMet55, βCys93, βCys112, βTyr130, βLys144, βHis146. The antioxidant potential of the Hb molecule in the intracellular space and when it enters the blood plasma is discussed.


About the authors

A. D. Vasilyeva

Institute of Biochemical Physics of the Russian Academy of Sciences

Author for correspondence.
Email: ms.kadaver@mail.ru

Russian Federation, 4, Kosygina street, Moscow, 119991

L. V. Yurina

Institute of Biochemical Physics of the Russian Academy of Sciences

Email: ms.kadaver@mail.ru

Russian Federation, 4, Kosygina street, Moscow, 119991

A. E. Bugrova

Institute of Biochemical Physics of the Russian Academy of Sciences

Email: ms.kadaver@mail.ru

Russian Federation, 4, Kosygina street, Moscow, 119991

M. I. Indeykina

Institute of Biochemical Physics of the Russian Academy of Sciences; Moscow Institute of Physics and Technology

Email: ms.kadaver@mail.ru

Russian Federation, 4, Kosygina street, Moscow, 119991; 9, Institutskij, Dolgoprudny, Moscow region, 141701

D. Y. Azarova

Institute of Biochemical Physics of the Russian Academy of Sciences; Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin

Email: ms.kadaver@mail.ru

Russian Federation, 4, Kosygina street, Moscow, 119991; 23, Academician Skryabin street , Moscow, 109472

A. V. Bychkova

Institute of Biochemical Physics of the Russian Academy of Sciences

Email: ms.kadaver@mail.ru

Russian Federation, 4, Kosygina street, Moscow, 119991

K. I. Akzhigitova

Institute of Biochemical Physics of the Russian Academy of Sciences; Belinsky pedagogical Institute of Penza State University

Email: ms.kadaver@mail.ru

Russian Federation, 4, Kosygina street, Moscow, 11999; 37, Lermontova street, Penza, 440026

A. S. Kononikhin

Institute of Biochemical Physics of the Russian Academy of Sciences; Talrose Institute for EnergyProblems of Chemical Physics of the Russian Academy of Sciences; Moscow Institute of Physics and Technology

Email: ms.kadaver@mail.ru

Russian Federation, 4, Kosygina street, Moscow, 119991; 38-2, Leninsky Prospekt, Moscow, 119334; 9, Institutskij, Dolgoprudny, Moscow region, 141701

E. N. Nikolaev

Institute of Biochemical Physics of the Russian Academy of Sciences; Talrose Institute for EnergyProblems of Chemical Physics of the Russian Academy of Sciences; Skolkovo Institute of Science and Technology

Email: ms.kadaver@mail.ru

Russian Federation, 4, Kosygina street, Moscow, 119991; 38-2, Leninsky Prospekt, Moscow, 119334;3, Nobelya street, Moscow, 143026

Corresponding Member of the Russian Academy of Sciences

M. A. Rosenfeld

Institute of Biochemical Physics of the Russian Academy of Sciences

Email: ms.kadaver@mail.ru

Russian Federation, 4, Kosygina street, Moscow, 119991

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