Email this article Multiplex PCR test-system development for the genotyping of transgenic mice with pBC1 MILK expression vector-based transgene
- Authors: Kalmykov V.A.1, Kusov P.A.1,2, Deykin A.V.1,3
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Affiliations:
- Institute of Gene Biology of the Russian Academy of Sciences
- Skolkovo Institute of Science and Technology
- Institute of General Pathology and Pathophysiology
- Issue: Vol 485, No 6 (2019)
- Pages: 750-753
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/14455
- DOI: https://doi.org/10.31857/S0869-56524856750-753
- ID: 14455
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Abstract
Developed multiplex PCR test-system for identification of transgenic cis-regulatory elements (promoter, insulator) from genetic constructions based on pBC1 Milk expression vector incorporated into the Mus musculus genome. Test-system was validated by genotyping producent strains generated by linearized vector DNA microinjection into fertilized zygote with occasional transgene genome integration, expressing the following transgenes in mammary gland, in milk: Human lactoferrin protein in different length, heat-shock protein HSP-70, Firefly luciferase protein, lysozyme protein. Reaction conditions selection described in details for better reproducibility.
About the authors
V. A. Kalmykov
Institute of Gene Biology of the Russian Academy of Sciences
Email: deikin@igb.ac.ru
Russian Federation, 34/5, Vavilova street, Moscow, 119334
P. A. Kusov
Institute of Gene Biology of the Russian Academy of Sciences; Skolkovo Institute of Science and Technology
Email: deikin@igb.ac.ru
Russian Federation, 34/5, Vavilova street, Moscow, 119334; 3, Nobelya street, Moscow, 143026
A. V. Deykin
Institute of Gene Biology of the Russian Academy of Sciences; Institute of General Pathology and Pathophysiology
Author for correspondence.
Email: deikin@igb.ac.ru
Russian Federation, 34/5, Vavilova street, Moscow, 119334; 8, Baltiyskaya st., Moscow, 125315
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