Email this article NAD(P)H: FMN-oxidoreductase functioning under macromolecular crowding: in vitro modeling
- Authors: Govorun A.E.1, Esimbekova E.N.1,2, Kratasyuk V.A.1,2
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Affiliations:
- Siberian Federal University
- Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science
- Issue: Vol 486, No 4 (2019)
- Pages: 500-503
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/14474
- DOI: https://doi.org/10.31857/S0869-56524864500-503
- ID: 14474
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Abstract
The functioning of Vibrio fischeri NAD(P)H: FMN-oxidoreductase (Red) under conditions of macromolecular crowding (MMC) modeled in vitro by adding biopolymers (starch and gelatin) was studied. The dissociation rate constants and the activation energies of dissociation of Red to the subunits were calculated; the process of denaturation of Red was analyzed. It was shown that the functioning of Red both under conditions of MMC and diluted solutions is the same. The result refutes the common belief that due to MMC the stabilization of enzymes’ native conformation occurs in vivo when compared to in vitro.
About the authors
A. E. Govorun
Siberian Federal University
Author for correspondence.
Email: aebezrukih@gmail.com
Russian Federation, 79, Svobodny avenue, Krasnoyarsk, 660041
E. N. Esimbekova
Siberian Federal University; Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science
Email: aebezrukih@gmail.com
Russian Federation, 79, Svobodny avenue, Krasnoyarsk, 660041; 50, Akademgorodok, Krasnoyarsk, 660036
V. A. Kratasyuk
Siberian Federal University; Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science
Email: aebezrukih@gmail.com
Russian Federation, 79, Svobodny avenue, Krasnoyarsk, 660041; 50, Akademgorodok, Krasnoyarsk, 660036
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