Bacteriochlorophyll interaction with singlet oxygen in membranes of purple photosynthetic bacteria: does the protective function of carotenoids exist?

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Abstract


The direct action of singlet oxygen on the bacteriochlorophyll (BChl) of light-harvesting complexes in the membranes of four types of purple non-sulfur and sulfur photosynthesizing bacteria with and without carotenoids has been studied. It has been found that BChl in carotenoid-less samples is generally more resistant to the action of singlet oxygen compared to the control. It is assumed that carotenoids are not needed to protect BChl of bacterial light-harvesting complexes from singlet oxygen, and in the classic work of Griffith et al. [1] the apoptosis process in carotenoid-less mutant cells, which involves the destruction of complexes, the appearance of monomeric BChl and generation of singlet oxygencaused by BChl, followed by BChl oxidation, was mistakenly attributed to the protective function of carotenoids.


About the authors

Z. K. Makhneva

Institute of Basic Biological Problems of the Russian Academy of Sciences

Email: AshikhminAA@gmail.com

Russian Federation, 2, Institutskaya street, Pushchino, Moscow Region, 142290

A. A. Ashikhmin

Institute of Basic Biological Problems of the Russian Academy of Sciences

Author for correspondence.
Email: AshikhminAA@gmail.com

Russian Federation, 2, Institutskaya street, Pushchino, Moscow Region, 142290

M. A. Bolshakov

Institute of Basic Biological Problems of the Russian Academy of Sciences

Email: AshikhminAA@gmail.com

Russian Federation, 2, Institutskaya street, Pushchino, Moscow Region, 142290

A. A. Moskalenko

Institute of Basic Biological Problems of the Russian Academy of Sciences

Email: andrey-moskalenko@rambler.ru

Russian Federation, 2, Institutskaya street, Pushchino, Moscow Region, 142290

References

  1. Griffith M., Sistrom W.R., Cohen-Bazire G., Stanier R.Y. Functions of Carotenoids in Photosynthesis // Nature. 1955. V. 176. P. 1211-1215.
  2. Cogdell R.J. and Frank H.A. How Carotenoids Function in Photosynthetic Bacteria // Biochem Biophys Acta. 1987. V. 895. P. 63-79.
  3. Arellano J.B., Yousef Y.A., Melø T.B., Mahamad S.B.B., Cogdell R.J., Naqvi K.R. Formation and Geminate Quenching of Singlet Oxygen in Purple Bacterial Reaction Center // J. Photochem. Photobiol. B. Biol. 2007. V. 87. P. 105-112.
  4. Uchoa A.F., Knox P.P., Turchielle R., Seifullina N.Kh., Baptista S.M. Singlet Oxygen Generation in the Reaction Centers of Rhodobacter sphaeroides // Eur. Biophys. J. 2008. V. 37. P. 843-850.
  5. Ashikhmin A., Makhneva Z., Moskalenko A. The LH2 Complexes are Assembled in the Cells of Purple Sulfur Bacterium Ectothiorhodospira haloalkaliphila with Inhibition of Carotenoid Biosynthesis // Photosynth. Res. 2014. V. 119. P. 291-303.
  6. Maisch T., Baier J., Franz B., Maier M., Landthaler M., Szeimies R-M., Bäumler W. The Role of Singlet Oxygen and Oxygen Concentration in Photodynamic Inactivation of Bacteria // Proc. Natl. Acad. Sci. USA. 2007. V. 104. P. 7223-7228.
  7. Bahatyrova S., Frese R.N., Siebert C.A., Olsen J.D., van der Werf K., van Grondelle O.R., van Niederman R.A., Bullough P.A., Otto C., Hunter C.N. The Native Architecture of a Photosynthetic Membrane // Nature. 2004. V. 430. P. 1058-1062.
  8. Löhner A., Carey A.M., Hacking K., Picken N., Kelly S., Cogdell R., Köhler J. The Origin of the Split B800 Absorption Peak in the LH2 Complexes from Allochromatium vinosum // Photosynth Res. 2015. V. 123. P. 23-31.
  9. Торопыгина О.А., Махнева З.К., Москаленко А.А. Кластерам бактериохлорофилла не требуются каротиноиды для защиты от фотоокисления в светособирающих комплексах фотосинтезирующих бактерий // ДАН. 2003. Т. 391. С. 828-831.
  10. Makhneva Z., Bolshakov M., Moskalenko A. Heterogeneity of Carotenoid Content and Composition in LH2 of the Sulphur Purple Bacterium Allochromatium minutissimum Grown under Carotenoid-Biosynthesis Inhibition // Photosynth. Res. 2008. V. 98. P. 633-641.
  11. Berghoff B.A., Glaeser J., Nuss A.M., Zobawa M., Lottspeich F., Klug G. Anoxygenic Photosynthesis and Photooxidative Stress: a Particular Challenge for Roseobacter // Environ. Microbiol. 2011. V. 13. P. 775-791.
  12. Cogdell R.J., Howard T.D., Bittl R., Schlodder E., Geisenheimer I., Lubitz W. How Carotenoids Protect Bacterial Photosynthesis // Philos. Trans. R. Soc. Lond B Biol. Sci. 2000. V. 355. Р. 1345-1349.
  13. Šlouf V., Cháber P., Olsen J.D., Martin E.C., Qian P., Hunter C.N., Polívka T. Photoprotection in a Purple Phototrophic Bacterium Mediated by Oxygen-Dependent Alteration of Carotenoid Excited-State Properties // Proc. Natl. Acad. Sci. USA. 2012. V. 109. P. 8570-8575.
  14. Dworkin M. Endogenous Photosensitization in a Carotenoidless Mutant of Rhodopseudomonas spheroides // J. Gen. Physiol. 1958. V. 41. P. 1099-1112.
  15. Limantara L., Koehler P., Wilhelm B., Porra R.J., Scheer H. Photostability of Bacteriochlorophyll a and Derivatives: Potential Sensitizers for Photodynamic Tumor Therapy // Photochem. Photobiol. 2006. V. 82. P. 770-780.

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