Email this article Functional comparison of short and long isoforms of the TRF2 protein in Drosophila melanogaster
- Authors: Osadchiy I.S.1, Georgiev P.G.1, Maksimenko O.G.1
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Affiliations:
- Institute of Gene Biology of the Russian Academy of Sciences
- Issue: Vol 486, No 4 (2019)
- Pages: 514-518
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/14483
- DOI: https://doi.org/10.31857/S0869-56524864514-518
- ID: 14483
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Abstract
The TRF2 protein (TBP-related factor 2) can substitute for TBP in the TFIID complex forming alternative transcription initiation complexes for TATA-less promoters, including promoters of histone H1 and required for transposon repression piRNA-clusters. The Drosophila trf2 gene codes for two isoforms: a “short” and a “long” one, in which the same short TRF2 sequence is preceded by a long N-terminal domain. Here, we demonstrated that the long TRF2 isoform has greater functional activity then the short isoform by expressing each of them in reduced rate under the endogenous promoters. Expression of the long isoform alone doesn't affect neither the flies` viability nor the sex ratio. Expression of the short isoform alone leads to the phenotype described for the trf2 gene insufficiency and derepression of transposable elements, that is, decreased viability, violation of homologous chromosome pairing and segregation, and apparent female-biased sex ratio.
About the authors
I. S. Osadchiy
Institute of Gene Biology of the Russian Academy of Sciences
Author for correspondence.
Email: untie@mail.ru
Russian Federation, 34/5, Vavilova street, Moscow, 119334
P. G. Georgiev
Institute of Gene Biology of the Russian Academy of Sciences
Email: untie@mail.ru
Academician of the Russian Academy of Sciences
Russian Federation, 34/5, Vavilova street, Moscow, 119334O. G. Maksimenko
Institute of Gene Biology of the Russian Academy of Sciences
Email: untie@mail.ru
Russian Federation, 34/5, Vavilova street, Moscow, 119334
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