Hypochlorite-induced damage of plasminogen molecules: structural-functional disturbance
- Authors: Vasilyeva A.D.1, Yurina L.V.1, Shchegolikhin A.N.1, Bugrova A.E.1, Konstantinova T.S.1, Indeykina M.I.1,2, Kononikhin A.S.1,2,3, Nikolaev E.N.1,3, Rosenfeld M.A.1
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Affiliations:
- Institute of Biochemical Physics of the Russian Academy of Sciences
- Moscow Institute of Physics and Technology
- Skolkovo Institute of Science and Technology
- Issue: Vol 488, No 5 (2019)
- Pages: 560-566
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/17831
- DOI: https://doi.org/10.31857/S0869-56524885560-566
- ID: 17831
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Full Text
Abstract
Plasminogen being a precursor of plasmin, a serine protease which plays a fundamental role in the intravascular thrombolysis. For the first time, by using high-resolution mass spectrometry, data were obtained of oxidative modifications of the plasminogen molecule under induced oxidation. The FTIR data show that under oxidation on the protein, its secondary structure also undergoes the rearrangements. The high tolerance of plasminogen to oxidation can be due to both the closed conformation and the ability of some Met residues to serve as ROS trap.
About the authors
A. D. Vasilyeva
Institute of Biochemical Physics of the Russian Academy of Sciences
Author for correspondence.
Email: alexandra.d.vasilyeva@gmail.com
Russian Federation, 4, Kosygina street, Moscow, 119991
L. V. Yurina
Institute of Biochemical Physics of the Russian Academy of Sciences
Email: alexandra.d.vasilyeva@gmail.com
Russian Federation, 4, Kosygina street, Moscow, 119991
A. N. Shchegolikhin
Institute of Biochemical Physics of the Russian Academy of Sciences
Email: alexandra.d.vasilyeva@gmail.com
Russian Federation, 4, Kosygina street, Moscow, 119991
A. E. Bugrova
Institute of Biochemical Physics of the Russian Academy of Sciences
Email: alexandra.d.vasilyeva@gmail.com
Russian Federation, 4, Kosygina street, Moscow, 119991
T. S. Konstantinova
Institute of Biochemical Physics of the Russian Academy of Sciences
Email: alexandra.d.vasilyeva@gmail.com
Russian Federation, 4, Kosygina street, Moscow, 119991
M. I. Indeykina
Institute of Biochemical Physics of the Russian Academy of Sciences; Moscow Institute of Physics and Technology
Email: alexandra.d.vasilyeva@gmail.com
Russian Federation, 4, Kosygina street, Moscow, 119991; 9, Institutskij, Dolgoprudny, Moscow region, 141701
A. S. Kononikhin
Institute of Biochemical Physics of the Russian Academy of Sciences; Moscow Institute of Physics and Technology; Skolkovo Institute of Science and Technology
Email: alexandra.d.vasilyeva@gmail.com
Russian Federation, 4, Kosygina street, Moscow, 119991; 9, Institutskij, Dolgoprudny, Moscow region, 141701; 3, Nobelya street, Moscow, 143026
E. N. Nikolaev
Institute of Biochemical Physics of the Russian Academy of Sciences; Skolkovo Institute of Science and Technology
Email: alexandra.d.vasilyeva@gmail.com
Corresponding Member of the Russian Academy of Sciences
Russian Federation, 4, Kosygina street, Moscow, 119991; 3, Nobelya street, Moscow, 143026M. A. Rosenfeld
Institute of Biochemical Physics of the Russian Academy of Sciences
Email: alexandra.d.vasilyeva@gmail.com
Russian Federation, 4, Kosygina street, Moscow, 119991
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